Abstract
The NAC gene family has transcription factors specific to plants, which are involved in development and stress response and adaptation. In this study, ZmNAC89, an NAC gene in maize that plays a role in saline–alkaline tolerance, was isolated and characterized. ZmNAC89 was localized in the nucleus and had transcriptional activation activity during in vitro experiments. The expression of ZmNAC89 was strongly upregulated under saline–alkaline, drought and ABA treatments. Overexpression of the ZmNAC89 gene in transgenic Arabidopsis and maize enhanced salt tolerance at the seedling stage. Differentially expressed genes (DEGs) were then confirmed via RNA-sequencing analysis with the transgenic maize line. GO analyses showed that oxidation–reduction process-regulated genes were involved in ZmNAC89-mediated salt–alkaline stress. ZmNAC89 may regulate maize saline–alkali tolerance through the REDOX pathway and ABA signal transduction pathway. From 140 inbred maize lines, 20 haplotypes and 16 SNPs were found in the coding region of the ZmNAC89 gene, including the excellent haplotype HAP20. These results contribute to a better understanding of the response mechanism of maize to salt–alkali stress and marker-assisted selection during maize breeding.
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Claims 13
Claim · model-written summary
ZmNAC89 was isolated and characterized as a maize saline–alkaline tolerance gene.
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Maize (Zea mays L.)”
e0“ZmNAC89, an NAC gene in maize that plays a role in saline–alkaline tolerance, was isolated and characterized”
Claim · model-written summary
ZmNAC89 localized in the nucleus.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Maize (Zea mays L.)”
e0“ZmNAC89 was localized in the nucleus”
Claim · model-written summary
ZmNAC89 showed transcriptional activation activity in vitro.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Maize (Zea mays L.)”
e0“ZmNAC89 was localized in the nucleus and had transcriptional activation activity during in vitro experiments”
Claim · model-written summary
Saline–alkaline treatment strongly upregulated ZmNAC89 expression.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Maize (Zea mays L.)”
e0“The expression of ZmNAC89 was strongly upregulated under saline–alkaline, drought and ABA treatments”
Claim · model-written summary
Drought treatment strongly upregulated ZmNAC89 expression.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Maize (Zea mays L.)”
e0“The expression of ZmNAC89 was strongly upregulated under saline–alkaline, drought and ABA treatments”
Claim · model-written summary
ABA treatment strongly upregulated ZmNAC89 expression.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Maize (Zea mays L.)”
e0“The expression of ZmNAC89 was strongly upregulated under saline–alkaline, drought and ABA treatments”
Claim · model-written summary
ZmNAC89 overexpression enhanced salt tolerance in transgenic Arabidopsis seedlings.
- Taxon
- Arabidopsis
Arabidopsise0 - Organ / stage
- seedling stagee0
- Treatment
- Overexpression of the ZmNAC89 genee0
- Gene
- ZmNAC89e0
- Outcome
- salt tolerancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of the ZmNAC89 gene in transgenic Arabidopsis and maize enhanced salt tolerance at the seedling stage”
Claim · model-written summary
ZmNAC89 overexpression enhanced salt tolerance in transgenic maize seedlings.
- Organ / stage
- seedling stagee0
- Treatment
- Overexpression of the ZmNAC89 genee0
- Gene
- ZmNAC89e0
- Outcome
- salt tolerancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of the ZmNAC89 gene in transgenic Arabidopsis and maize enhanced salt tolerance at the seedling stage”
Claim · model-written summary
GO analysis implicated oxidation–reduction process genes in ZmNAC89-mediated saline–alkaline stress.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Maize (Zea mays L.)”
e0“GO analyses showed that oxidation–reduction process-regulated genes were involved in ZmNAC89-mediated salt–alkaline stress”
Claim · model-written summary
ZmNAC89 may regulate maize saline–alkali tolerance through REDOX and ABA signaling pathways.
- Gene
- ZmNAC89e0
- Outcome
- saline–alkali tolerancee0
- Qualifier
- REDOX pathway and ABA signal transduction pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ZmNAC89 may regulate maize saline–alkali tolerance through the REDOX pathway and ABA signal transduction pathway”
Claim · model-written summary
Twenty haplotypes were found in the ZmNAC89 coding region among 140 inbred maize lines.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“From 140 inbred maize lines, 20 haplotypes and 16 SNPs were found in the coding region of the ZmNAC89 gene”
Claim · model-written summary
Sixteen SNPs were found in the ZmNAC89 coding region among 140 inbred maize lines.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“From 140 inbred maize lines, 20 haplotypes and 16 SNPs were found in the coding region of the ZmNAC89 gene”
Claim · model-written summary
HAP20 was identified as an excellent ZmNAC89 haplotype.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Maize (Zea mays L.)”
e0“including the excellent haplotype HAP20”
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See all 65 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.