Abstract
Copper (Cu) is essential for plant growth and development. IRON MAN (IMA) is a family of small peptides that can bind both iron (Fe) and Cu ions. It was reported that IMAs mediate Fe homeostasis in Arabidopsis thaliana. However, it remains unclear whether IMAs are involved in Cu homeostasis. The transcript abundance of IMA genes decreased in response to Cu deficiency. The combined disruption of all IMA genes caused enhanced tolerance to Cu deficiency and resulted in an increase in the transcript abundance of Cu uptake genes, whereas the overexpression of IMA1 or IMA3 led to the opposite results. Protein interaction assays indicated that IMAs interact with Cu-DEFICIENCY INDUCED TRANSCRIPTION FACTOR1 (CITF1), which is a positive regulator of the Cu uptake genes. Further studies showed that IMAs not only interfere with the DNA binding of CITF1 but also repress the transcriptional activation activity of CITF1, hence resulting in downregulation of the Cu uptake genes. Genetic analyses indicated that IMAs modulate Cu homeostasis in a CITF1-dependent manner. Our findings indicate that IMAs inhibit the functions of CITF1 in regulating Cu deficiency responses, thereby providing a conceptual framework for comprehending the regulation of Cu homeostasis.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
IMA gene transcript abundance decreased in Arabidopsis in response to copper deficiency.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae1 - Treatment
- Cu deficiencye2
- Gene
- IMA genese2
- Outcome
- transcript abundance of IMA genese2
- Direction
- decreasede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“mediate Fe homeostasis in Arabidopsis thaliana”
e2“The transcript abundance of IMA genes decreased in response to Cu deficiency”
Claim · model-written summary
Combined disruption of all IMA genes enhanced tolerance to copper deficiency in Arabidopsis.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- combined disruption of all IMA genese2
- Gene
- IMA genese2
- Outcome
- tolerance to Cu deficiencye2
- Direction
- enhancede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“mediate Fe homeostasis in Arabidopsis thaliana”
e2“The combined disruption of all IMA genes caused enhanced tolerance to Cu deficiency and resulted in an increase in the transcript abundance of Cu uptake genes, whereas the overexpression of IMA1 or IMA3 led to the opposite results”
Claim · model-written summary
Combined disruption of all IMA genes increased the transcript abundance of Cu uptake genes.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- combined disruption of all IMA genese2
- Outcome
- transcript abundance of Cu uptake genese2
- Direction
- increasee2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“mediate Fe homeostasis in Arabidopsis thaliana”
e2“The combined disruption of all IMA genes caused enhanced tolerance to Cu deficiency and resulted in an increase in the transcript abundance of Cu uptake genes, whereas the overexpression of IMA1 or IMA3 led to the opposite results”
Claim · model-written summary
Overexpression of IMA1 or IMA3 led to the opposite results, reducing copper deficiency tolerance and Cu uptake gene expression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- overexpression of IMA1 or IMA3e2
- Gene
- IMA1 or IMA3e2
- Outcome
- the opposite resultse2
- Direction
- decreaseinferrede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“mediate Fe homeostasis in Arabidopsis thaliana”
e2“The combined disruption of all IMA genes caused enhanced tolerance to Cu deficiency and resulted in an increase in the transcript abundance of Cu uptake genes, whereas the overexpression of IMA1 or IMA3 led to the opposite results”
Claim · model-written summary
IMAs interact with CITF1 in Arabidopsis.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- CITF1e2
- Outcome
- IMAs interact with Cu-DEFICIENCY INDUCED TRANSCRIPTION FACTOR1 (CITF1)e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“mediate Fe homeostasis in Arabidopsis thaliana”
e2“Protein interaction assays indicated that IMAs interact with Cu-DEFICIENCY INDUCED TRANSCRIPTION FACTOR1 (CITF1), which is a positive regulator of the Cu uptake genes”
Claim · model-written summary
IMAs interfere with the DNA binding of CITF1 in Arabidopsis.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- CITF1e2
- Outcome
- DNA binding of CITF1e2
- Qualifier
- interferee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“mediate Fe homeostasis in Arabidopsis thaliana”
e2“Further studies showed that IMAs not only interfere with the DNA binding of CITF1 but also repress the transcriptional activation activity of CITF1”
Claim · model-written summary
IMAs repress the transcriptional activation activity of CITF1 in Arabidopsis.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- CITF1e2
- Outcome
- transcriptional activation activity of CITF1e2
- Qualifier
- represse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“mediate Fe homeostasis in Arabidopsis thaliana”
e2“Further studies showed that IMAs not only interfere with the DNA binding of CITF1 but also repress the transcriptional activation activity of CITF1”
Claim · model-written summary
IMAs modulate copper homeostasis in a CITF1-dependent manner.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- CITF1e2
- Outcome
- Cu homeostasise2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“mediate Fe homeostasis in Arabidopsis thaliana”
e2“Genetic analyses indicated that IMAs modulate Cu homeostasis in a CITF1-dependent manner”
The same questions in other papers
iron atom · disease · Arabidopsis thaliana (thale cress)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
loss of function · disease · Arabidopsis thaliana (thale cress)Positive and negative results
30 papers in the same direction as this paper · 51 in the opposite direction
- negative
- negative
- negative
78 papers more in this group
See all 82 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.