Abstract
The expression of cereal grain storage protein (GSP) genes is controlled by a complex network of transcription factors (TFs). Storage protein activator (SPA) is a major TF acting in this network but its specific function in wheat (Triticum aestivum L.) remains to be determined. Here we generated an RNAi line in which expression of the three SPA homoeologs was reduced. In this line and its null segregant we analyzed GSP accumulation and expression of GSP and regulatory TF genes under two regimes of nitrogen availability. We show that down regulation of SPA decreases grain protein concentration at maturity under low but not high nitrogen supply. Under low nitrogen supply, the decrease in SPA expression also caused a reduction in the total quantity of GSP per grain and in the ratio of GSP to albumin-globulins, without significantly affecting GSP composition. The slight reduction in GSP gene expression measured in the SPA RNAi line under low nitrogen supply did not entirely account for the more significant decrease in GSP accumulation, suggesting that SPA regulates additional levels of GSP synthesis. Our results demonstrate a clear role of SPA in the regulation of grain nitrogen metabolism when nitrogen is a limiting resource.
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Claims 5
Claim · model-written summary
Down-regulation of SPA decreased grain protein concentration at maturity in bread wheat under low but not high nitrogen supply.
- Taxon
- bread wheat
Triticum aestivumt0 - Treatment
- down regulation of SPAe0
- Gene
- SPAe0
- Outcome
- grain protein concentratione0
- Direction
- decreasese0
- Qualifier
- under low but not high nitrogen supplye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in bread wheat”
e0“We show that down regulation of SPA decreases grain protein concentration at maturity under low but not high nitrogen supply”
Claim · model-written summary
Under low nitrogen supply, SPA down-regulation reduced the total quantity of GSP per grain in bread wheat.
- Taxon
- bread wheatTriticum aestivumt0
- Treatment
- the decrease in SPA expressione1
- Gene
- SPAe1
- Outcome
- total quantity of GSP per graine1
- Direction
- reductione1
- Qualifier
- Under low nitrogen supplye1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in bread wheat”
e1“Under low nitrogen supply, the decrease in SPA expression also caused a reduction in the total quantity of GSP per grain and in the ratio of GSP to albumin-globulins, without significantly affecting GSP composition”
Claim · model-written summary
Under low nitrogen supply, SPA down-regulation reduced the ratio of GSP to albumin-globulins in bread wheat.
- Taxon
- bread wheatTriticum aestivumt0
- Treatment
- the decrease in SPA expressione1
- Gene
- SPAe1
- Outcome
- ratio of GSP to albumin-globulinse1
- Direction
- reductione1
- Qualifier
- Under low nitrogen supplye1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in bread wheat”
e1“Under low nitrogen supply, the decrease in SPA expression also caused a reduction in the total quantity of GSP per grain and in the ratio of GSP to albumin-globulins, without significantly affecting GSP composition”
Claim · model-written summary
Under low nitrogen supply, SPA down-regulation did not significantly affect GSP composition in bread wheat.
- Taxon
- bread wheatTriticum aestivumt0
- Treatment
- the decrease in SPA expressione1
- Gene
- SPAe1
- Outcome
- GSP compositione1
- Direction
- without significantly affectinge1
- Qualifier
- Under low nitrogen supplye1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in bread wheat”
e1“Under low nitrogen supply, the decrease in SPA expression also caused a reduction in the total quantity of GSP per grain and in the ratio of GSP to albumin-globulins, without significantly affecting GSP composition”
Claim · model-written summary
SPA regulates additional levels of GSP synthesis beyond GSP gene expression in bread wheat, as suggested by the authors.
- Taxon
- bread wheatTriticum aestivumt0
- Gene
- SPAe2
- Outcome
- additional levels of GSP synthesise2
- Qualifier
- did not entirely account for the more significant decrease in GSP accumulatione2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in bread wheat”
e2“The slight reduction in GSP gene expression measured in the SPA RNAi line under low nitrogen supply did not entirely account for the more significant decrease in GSP accumulation, suggesting that SPA regulates additional levels of GSP synthesis”
The same questions in other papers
storage · quality: protein · Triticum aestivum (wheat)Positive and negative results
5 papers in the same direction as this paper · 4 in the opposite direction
- positive
- positive
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See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.