Abstract
Drought is one of the key abiotic stresses that critically influences the crops by restraining their growth and yield potential. Being sessile, plant tackle the detrimental effects of drought stress by modulating the cellular state by changing the gene expression. The transcriptional syndicate essentially drives such alteration of gene expression. Transcription factors (TF) are the key regulatory protein that controls the expression of their target gene by binding to the cis-regulatory elements present in the promoter region. Myb-TF, ubiquitously present in all eukaryotes belong to one of the largest TF family, and play a wide array of biological functions in plants, including anthocyanin biosynthesis, vasculature system, cell signalling, seed maturation and abiotc stress responses. The present performed isolation and molecular cloning of full length Myb TF from Eleusine corocana. The isolated full-length coding sequence has 1053 bp and 350 aa was submitted to NCBI (Accession number MT312253). The transcript level of EcMYB increases under different abiotic stress treatments including dehydration, salinity, and high-temperature stress. The promoter region of EcMyb1 was found to be enriched in stress-responsive cis-regulatory elements such as DRE, HSE, ABRE etc. In phylogenetic analysis, EcMyb1 appeared to have high homology with its monocot orthologs particularly Sateria italica, Hordeum vulgare, Saccharum barberi and Oryza sativa. The three-dimension protein structure was generated based on homology modeling and structural aspects were discussed. Further, Insilco analysis was conducted to explore the physiological properties, subcellular localization, potential posttranslational modification sites (phosphorylation and glycosylation sites), and molecular and biological function of the full-length protein. Overall, the expression profiling and Insilco analysis of EcMyb1 strongly indicated its potential role in abiotic stress response in Eleusine corocana.
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Claims 5
Claim · model-written summary
EcMYB transcript level increases under dehydration, salinity and high-temperature stress.
- Taxon
- Eleusine coracana
Eleusine coracanat0 - Treatment
- dehydration, salinity, and high-temperature stresse0
- Gene
- EcMYBe0
- Outcome
- transcript levele0
- Direction
- increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“C4 plant Eleusine coracana”
e0“The transcript level of EcMYB increases under different abiotic stress treatments including dehydration, salinity, and high-temperature stress”
Claim · model-written summary
The EcMyb1 promoter is enriched in stress-responsive cis-regulatory elements.
- Taxon
- Eleusine coracanaEleusine coracanat0
- Gene
- EcMyb1e1
- Outcome
- stress-responsive cis-regulatory elementse1
- Direction
- enrichede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“C4 plant Eleusine coracana”
e1“The promoter region of EcMyb1 was found to be enriched in stress-responsive cis-regulatory elements such as DRE, HSE, ABRE etc”
Claim · model-written summary
EcMyb1 has high homology with its monocot orthologs.
- Taxon
- Eleusine coracanaEleusine coracanat0
- Gene
- EcMyb1e2
- Outcome
- homology with its monocot orthologse2
- Direction
- highe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“C4 plant Eleusine coracana”
e2“EcMyb1 appeared to have high homology with its monocot orthologs”
Claim · model-written summary
The full-length EcMyb1 coding sequence is 1053 bp and 350 aa.
- Taxon
- Eleusine coracanaEleusine coracanat0
- Outcome
- full-length coding sequencee3
- Value
- 1053 bp and 350 aae3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“C4 plant Eleusine coracana”
e3“The isolated full-length coding sequence has 1053 bp and 350 aa”
Claim · model-written summary
Expression profiling and in-silico analysis indicate that EcMyb1 has a potential role in abiotic stress response.
- Taxon
- Eleusine coracanaEleusine coracanat0
- Gene
- EcMyb1e4
- Outcome
- potential role in abiotic stress responsee4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“C4 plant Eleusine coracana”
e4“expression profiling and Insilco analysis of EcMyb1 strongly indicated its potential role in abiotic stress response”
The same questions in other papers
heat · molecular: expression · Eleusine coracana (finger millet)Results agree
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- positive
salinity · molecular: expression · Eleusine coracana (finger millet)Results agree
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- positive
- positive
- positive
drought · molecular: expression · Eleusine coracana (finger millet)Results agree
5 papers in the same direction as this paper
- positive
- positive
- positive
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See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.