Abstract
Grapevine (Vitis vinifera) is one of the major economic fruit crops but suffers many diseases, causing damage to the quality of grapes. Strain G166 was isolated from the rhizosphere of grapevine and was found to exhibited broad-spectrum antagonistic activities against fungal pathogens on grapes in vitro, such as Coniella diplodiella, Botrytis cinerea, and Colletotrichum gloeosporioides. Whole-genome sequencing revealed that G166 contained a 6,613,582 bp circular chromosome with 5749 predicted coding DNA sequences and an average GC content of 60.57%. TYGS analysis revealed that G166 belongs to Pseudomonas viciae. Phenotype analysis indicated that P. viciae G166 remarkably reduced the severity of grape white rot disease in the grapevine. After inoculation with C. diplodiella, more H2O2 and MDA accumulated in the leaves and resulted in decreases in the Pn and chlorophyll content. Conversely, G166-treated grapevine displayed less oxidative damage with lower H2O2 levels and MDA contents under the pathogen treatments. Subsequently, G166-treated grapevine could sustain a normal Pn and chlorophyll content. Moreover, the application of P. viciae G166 inhibited the growth of mycelia on detached leaves and berries, while more disease symptoms occurred in non-bacterized leaves and berries. Therefore, P. viciae G166 served as a powerful bioagent against grape white rot disease. Using antiSMASH prediction and genome comparisons, a relationship between non-ribosomal peptide synthase clusters and antifungal activity was found in the genome of P. viciae G166. Taken together, P. viciae G166 shows promising antifungal potential to improve fruit quality and yield in ecological agriculture.
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Claims 13
Claim · model-written summary
G166 antagonized Coniella diplodiella in vitro.
- Interactor
- Coniella diplodiellaConiella diplodiellae0pathogen
- Second interactor
- Strain G166e0biocontrol_agent
- Outcome
- antagonistic activities against fungal pathogense0
VitisOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Strain G166 was isolated from the rhizosphere of grapevine and was found to exhibited broad-spectrum antagonistic activities against fungal pathogens on grapes in vitro, such as Coniella diplodiella, Botrytis cinerea, and Colletotrichum gloeosporioides”
Claim · model-written summary
G166 antagonized Botrytis cinerea in vitro.
- Interactor
- Botrytis cinerea
Botrytis cinereae0pathogen - Second interactor
- Strain G166e0biocontrol_agent
- Outcome
- antagonistic activities against fungal pathogense0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Strain G166 was isolated from the rhizosphere of grapevine and was found to exhibited broad-spectrum antagonistic activities against fungal pathogens on grapes in vitro, such as Coniella diplodiella, Botrytis cinerea, and Colletotrichum gloeosporioides”
Claim · model-written summary
G166 antagonized Colletotrichum gloeosporioides in vitro.
- Interactor
- Colletotrichum gloeosporioides
Colletotrichum gloeosporioidese0pathogen - Second interactor
- Strain G166e0biocontrol_agent
- Outcome
- antagonistic activities against fungal pathogense0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Strain G166 was isolated from the rhizosphere of grapevine and was found to exhibited broad-spectrum antagonistic activities against fungal pathogens on grapes in vitro, such as Coniella diplodiella, Botrytis cinerea, and Colletotrichum gloeosporioides”
Claim · model-written summary
G166 has a 6,613,582 bp chromosome with 5749 predicted coding sequences and 60.57% GC.
- Outcome
- circular chromosome with 5749 predicted coding DNA sequences and an average GC contente0
- Value
- 6,613,582 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Whole-genome sequencing revealed that G166 contained a 6,613,582 bp circular chromosome with 5749 predicted coding DNA sequences and an average GC content of 60.57%”
t0“Grapevine”
Claim · model-written summary
TYGS placed G166 in Pseudomonas viciae.
- Interactor
- Pseudomonas viciaePseudomonas viciaee0biocontrol_agent
- Outcome
- G166 belongs to Pseudomonas viciaee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TYGS analysis revealed that G166 belongs to Pseudomonas viciae”
t0“Grapevine”
Claim · model-written summary
P. viciae G166 reduced grape white rot severity.
- Treatment
- P. viciae G166e0
- Interactor
- P. viciae G166Pseudomonas viciaee0biocontrol_agent
- Outcome
- severity of grape white rot diseasee0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“P. viciae G166 remarkably reduced the severity of grape white rot disease in the grapevine”
Claim · model-written summary
C. diplodiella inoculation raised leaf H2O2 and MDA.
- Treatment
- inoculation with C. diplodiellae0
- Interactor
- C. diplodiellaConiella diplodiellae0pathogen
- Outcome
- H2O2 and MDA accumulated in the leavese0
- Direction
- moree0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After inoculation with C. diplodiella, more H2O2 and MDA accumulated in the leaves”
t0“Grapevine”
Claim · model-written summary
C. diplodiella inoculation lowered Pn and chlorophyll.
- Treatment
- inoculation with C. diplodiellae0
- Interactor
- C. diplodiellaConiella diplodiellae0pathogen
- Outcome
- Pn and chlorophyll contente0
- Direction
- decreasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After inoculation with C. diplodiella, more H2O2 and MDA accumulated in the leaves and resulted in decreases in the Pn and chlorophyll content”
t0“Grapevine”
Claim · model-written summary
G166 treatment lowered H2O2 and MDA under pathogen treatment.
- Treatment
- G166-treatede0
- Interactor
- G166e0biocontrol_agent
- Outcome
- H2O2 levels and MDA contentse0
- Direction
- lowere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“G166-treated grapevine displayed less oxidative damage with lower H2O2 levels and MDA contents under the pathogen treatments”
Claim · model-written summary
G166 treatment sustained normal Pn and chlorophyll.
- Treatment
- G166-treatede0
- Interactor
- G166e0biocontrol_agent
- Outcome
- Pn and chlorophyll contente0
- Direction
- normale0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“G166-treated grapevine could sustain a normal Pn and chlorophyll content”
Claim · model-written summary
P. viciae G166 inhibited fungal mycelial growth on detached leaves and berries.
- Organ / stage
- detached leaves and berriese0
- Treatment
- application of P. viciae G166e0
- Interactor
- P. viciae G166Pseudomonas viciaee0biocontrol_agent
- Outcome
- growth of myceliae0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the application of P. viciae G166 inhibited the growth of mycelia on detached leaves and berries”
t0“Grapevine”
Claim · model-written summary
Non-bacterized leaves and berries had more disease symptoms.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“more disease symptoms occurred in non-bacterized leaves and berries”
t0“Grapevine”
Claim · model-written summary
Non-ribosomal peptide synthase clusters were associated with antifungal activity in G166.
- Interactor
- P. viciae G166Pseudomonas viciaee0biocontrol_agent
- Gene
- non-ribosomal peptide synthase clusterse0
- Outcome
- antifungal activitye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a relationship between non-ribosomal peptide synthase clusters and antifungal activity was found in the genome of P. viciae G166”
t0“Grapevine”
The same questions in other papers
inoculation · physiology: oxidative · VitisPositive and negative results
2 in the opposite direction
- positive
- positive
inoculation · physiology: photosynthesis · VitisPositive and negative results
4 in the opposite direction
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.