Abstract
Rice (Oryza sativa L.) is susceptible to various abiotic stresses, such as salt, cold, and drought. Therefore, there is an urgent need to explore the relevant genes that enhance tolerance to these stresses. In this study, we identified a gene, OsNCED4 (9-cis-epoxycarotenoid dioxygenase 4), which regulates tolerance to multiple abiotic stresses. OsNCED4 encodes a chloroplast-localized abscisic acid (ABA) biosynthetic enzyme. The expression of OsNCED4 gene was significantly induced by 150 mM NaCl and cold stress. Disruption of OsNCED4 by CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas9-mediated mutagenesis resulted in significant sensitivity to NaCl and cold stress. The salt and cold sensitivity of osnced4 mutant was due to the reduction of ABA content and the excessive accumulation of reactive oxygen species (ROS) under stress. Moreover, OsNCED4 also regulates drought stress tolerance of rice seedlings. Taken together, these results indicate that OsNCED4 is a new regulator for multiple abiotic stress tolerance in rice, and provided a potential target gene for enhancing multiple stress tolerance in the future.
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Claims 9
Claim · model-written summary
OsNCED4 was identified as a regulator of multiple abiotic stress tolerance.
- Taxon
- Rice
Oryza sativae1 - Gene
- OsNCED4e0
- Outcome
- tolerance to multiple abiotic stressese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified a gene, OsNCED4 (9-cis-epoxycarotenoid dioxygenase 4), which regulates tolerance to multiple abiotic stresses.”
e1“Rice (Oryza sativa L.)”
Claim · model-written summary
OsNCED4 encodes a chloroplast localized ABA biosynthetic enzyme.
- Taxon
- RiceOryza sativae1
- Gene
- OsNCED4e0
- Outcome
- chloroplast-localized abscisic acid (ABA) biosynthetic enzymee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsNCED4 encodes a chloroplast-localized abscisic acid (ABA) biosynthetic enzyme.”
e1“Rice (Oryza sativa L.)”
Claim · model-written summary
150 mM NaCl significantly induced OsNCED4 expression.
- Taxon
- RiceOryza sativae1
- Treatment
- 150 mM NaCle0
- Gene
- OsNCED4e0
- Outcome
- expression of OsNCED4 genee0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of OsNCED4 gene was significantly induced by 150 mM NaCl and cold stress.”
e1“Rice (Oryza sativa L.)”
Claim · model-written summary
Cold stress significantly induced OsNCED4 expression.
- Taxon
- RiceOryza sativae1
- Treatment
- cold stresse0
- Gene
- OsNCED4e0
- Outcome
- expression of OsNCED4 genee0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of OsNCED4 gene was significantly induced by 150 mM NaCl and cold stress.”
e1“Rice (Oryza sativa L.)”
Claim · model-written summary
CRISPR disruption of OsNCED4 caused significant NaCl sensitivity.
- Taxon
- RiceOryza sativae1
- Treatment
- Disruption of OsNCED4 by CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas9-mediated mutagenesise0
- Gene
- OsNCED4e0
- Outcome
- sensitivity to NaCle0
- Direction
- sensitivitye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Disruption of OsNCED4 by CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas9-mediated mutagenesis resulted in significant sensitivity to NaCl and cold stress.”
e1“Rice (Oryza sativa L.)”
Claim · model-written summary
CRISPR disruption of OsNCED4 caused significant cold sensitivity.
- Taxon
- RiceOryza sativae1
- Treatment
- Disruption of OsNCED4 by CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas9-mediated mutagenesise0
- Gene
- OsNCED4e0
- Outcome
- sensitivity to NaCl and cold stresse0
- Direction
- sensitivitye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Disruption of OsNCED4 by CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas9-mediated mutagenesis resulted in significant sensitivity to NaCl and cold stress.”
e1“Rice (Oryza sativa L.)”
Claim · model-written summary
Reduced ABA content was attributed as a cause of mutant salt and cold sensitivity.
- Taxon
- riceOryza sativae1
- Material
- osnced4 mutante0
- Compound
- ABAe0
- Outcome
- salt and cold sensitivitye0
- Direction
- reductione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The salt and cold sensitivity of osnced4 mutant was due to the reduction of ABA content”
e1“rice seedlings”
Claim · model-written summary
Excess ROS accumulation was attributed as a cause of mutant salt and cold sensitivity.
- Taxon
- riceOryza sativae1
- Material
- osnced4 mutante0
- Compound
- reactive oxygen species (ROS)e0
- Outcome
- salt and cold sensitivitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The salt and cold sensitivity of osnced4 mutant was due to the reduction of ABA content and the excessive accumulation of reactive oxygen species (ROS) under stress.”
e1“rice seedlings”
Claim · model-written summary
OsNCED4 also regulates rice seedling drought tolerance.
- Taxon
- riceOryza sativae0
- Organ / stage
- seedlingse0
- Gene
- OsNCED4e0
- Outcome
- drought stress tolerancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsNCED4 also regulates drought stress tolerance of rice seedlings.”
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