Abstract
Abstract Sugar transporters play a crucial role in plant responses to environmental factors. During plant–pathogen interactions, it is well established that sugar transporters and cell wall invertases are essential for regulating sugar availability at the plant–pathogen interface, impacting both plant resistance and pathogen proliferation. Despite these insights, their role in grapevine defence against pathogens remains underexplored. We examined the expression of sugar transporter and invertase genes in grape leaves infected with the necrotrophic fungus Botrytis cinerea . Our results highlighted significant coordinated upregulation of VvHT5 , VvcwINV and defence genes, suggesting a role in enhancing sink strength in infected leaves and implementing host defences. Heterologous expression of GFP‐fused proteins confirmed VvHT5 as a plasma membrane‐localized hexose symporter and phylogenetic analysis indicated its close relation with STP13‐like proteins, which are known to be implicated in host resistance across several plant–pathogen interactions. VvHT5 was heterologously expressed in Arabidopsis thaliana , resulting in high constitutive expression of the VvHT5 protein and increased glucose uptake activity. Phenotypic analysis revealed that VvHT5 enhanced basal resistance to B . cinerea and rescued the wild‐type phenotype in STP13‐deficient plants, indicating that VvHT5 is the grapevine orthologue of AtSTP13. Our findings suggest that VvHT5 may facilitate the reabsorption of extracellular monosaccharides, released from VvcwINV activity or damaged tissues during infection. This activity allows host cells to compete with necrotrophic pathogens for extracellular hexoses, thereby restricting sugar availability to the fungus. It would also support host metabolic demands for defence or serve as a signalling mechanism to orchestrate intracellular processes.
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Claims 9
Claim · model-written summary
Botrytis infection significantly upregulated VvHT5, VvcwINV and defence genes in grape leaves.
- Taxon
- grapee1
- Interactor
- Botrytis cinerea
Botrytis cinereae1pathogen - Gene
- VvHT5e0
- Outcome
- coordinated upregulatione0
- Direction
- upregulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“significant coordinated upregulation of VvHT5 , VvcwINV and defence genes”
e1“grape leaves infected with the necrotrophic fungus Botrytis cinerea”
Claim · model-written summary
VvHT5 was confirmed as a plasma membrane localized hexose symporter.
Verbatim
e0“confirmed VvHT5 as a plasma membrane‐localized hexose symporter”
t0“grape”
Claim · model-written summary
Phylogenetic analysis placed VvHT5 close to STP13 like proteins.
Verbatim
e0“phylogenetic analysis indicated its close relation with STP13‐like proteins”
e1“VvHT5 was heterologously expressed in Arabidopsis thaliana”
t0“grape”
Claim · model-written summary
Heterologous VvHT5 expression yielded high constitutive protein expression in Arabidopsis.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Treatment
- VvHT5 was heterologously expressede0
- Gene
- VvHT5e0
- Outcome
- constitutive expression of the VvHT5 proteine0
- Direction
- highe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“VvHT5 was heterologously expressed in Arabidopsis thaliana , resulting in high constitutive expression of the VvHT5 protein and increased glucose uptake activity.”
Claim · model-written summary
Heterologous VvHT5 expression increased glucose uptake in Arabidopsis.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- VvHT5 was heterologously expressede0
- Gene
- VvHT5e0
- Outcome
- glucose uptake activitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“VvHT5 was heterologously expressed in Arabidopsis thaliana , resulting in high constitutive expression of the VvHT5 protein and increased glucose uptake activity.”
Claim · model-written summary
VvHT5 enhanced basal resistance to B. cinerea in Arabidopsis.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- VvHT5e0
- Interactor
- B . cinereaBotrytis cinereae0pathogen
- Outcome
- basal resistance to B . cinereae0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“VvHT5 enhanced basal resistance to B . cinerea and rescued the wild‐type phenotype in STP13‐deficient plants”
e1“Arabidopsis thaliana”
Claim · model-written summary
VvHT5 rescued the wild type phenotype of STP13 deficient plants.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- VvHT5e0
- Outcome
- wild‐type phenotypee0
- Comparator
- STP13‐deficient plantse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“VvHT5 enhanced basal resistance to B . cinerea and rescued the wild‐type phenotype in STP13‐deficient plants”
e1“Arabidopsis thaliana”
Claim · model-written summary
The authors infer VvHT5 may reabsorb extracellular monosaccharides during infection.
Verbatim
e0“VvHT5 may facilitate the reabsorption of extracellular monosaccharides, released from VvcwINV activity or damaged tissues during infection.”
t0“grape”
Claim · model-written summary
The authors suggest sugar reabsorption restricts sugar availability to the fungus.
Verbatim
e0“This activity allows host cells to compete with necrotrophic pathogens for extracellular hexoses, thereby restricting sugar availability to the fungus.”
t0“grape”