Abstract
The application of synthetic fungicides has resulted in environmental pollution and adverse effects on non-target species. To reduce the use of agrochemicals, crop disease management requires microbial biological control agents. Bacillus-related genera produce secondary metabolites to control fungal pathogens. Bacillus velezensis GYUN-1190, isolated from soil, showed antagonistic activity against Colletotrichum fructicola, the apple anthracnose pathogen. Volatile organic compounds and culture filtrate (CF) from GYUN-1190 inhibited C. fructicola growth in vitro, by 80.9% and 30.25%, respectively. The CF of GYUN-1190 inhibited pathogen spore germination more than cell suspensions at 10 8 cfu/ml. Furthermore, GYUN-1190 CF is effective in inhibiting C. fructicola mycelial growth in vitro, and it suppresses apple fruit bitter rot more effectively than GYUN-1190 cell suspensions and pyraclostrobin in planta. The mycelial growth of C. fructicola was completely inhibited 48 h after immersion into the CF, in compared with positive controls and GYUN-1190 cell suspensions. The genetic mechanism underlying the biocontrol features of GYUN-1190 was defined using its whole-genome sequence, which was closely compared to similar strains. It consisted of 4,240,653 bp with 45.9% GC content, with 4,142 coding sequences, 87 tRNA, and 28 rRNA genes. The genomic investigation found 14 putative secondary metabolite biosynthetic gene clusters. The investigation suggests that B. velezensis GYUN-1190 might be more effective than chemical fungicides and could address its potential as a biological control agent.
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Claims 6
Claim · model-written summary
GYUN-1190 showed antagonism against the apple anthracnose pathogen.
- Taxon
- Apple
Malus domesticat0 - Interactor
- Colletotrichum fructicola
_Figure_8.jpg?width=60)
Colletotrichum fructicolae0pathogen - Second interactor
- Bacillus velezensis GYUN-1190
Bacillus velezensise0biocontrol_agent - Outcome
- antagonistic activity against Colletotrichum fructicolae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Figure_8.jpg?width=500)
Verbatim
e0“Bacillus velezensis GYUN-1190, isolated from soil, showed antagonistic activity against Colletotrichum fructicola, the apple anthracnose pathogen.”
t0“Apple”
Claim · model-written summary
GYUN-1190 volatiles inhibited pathogen growth in vitro by 80.9%.
- Taxon
- AppleMalus domesticat0
- Treatment
- Volatile organic compoundse0
- Interactor
- C. fructicolaColletotrichum fructicolae0
- Outcome
- C. fructicola growthe0
- Direction
- inhibitede0
- Value
- 80.9%e0
Not reportedInteractor roleComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Volatile organic compounds and culture filtrate (CF) from GYUN-1190 inhibited C. fructicola growth in vitro, by 80.9% and 30.25%, respectively.”
t0“Apple”
Claim · model-written summary
GYUN-1190 culture filtrate inhibited pathogen growth in vitro by 30.25%.
- Taxon
- AppleMalus domesticat0
- Treatment
- culture filtrate (CF)e0
- Interactor
- C. fructicolaColletotrichum fructicolae0
- Outcome
- C. fructicola growthe0
- Direction
- inhibitede0
- Value
- 30.25%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Volatile organic compounds and culture filtrate (CF) from GYUN-1190 inhibited C. fructicola growth in vitro, by 80.9% and 30.25%, respectively.”
t0“Apple”
Claim · model-written summary
GYUN-1190 filtrate inhibited spore germination more than cell suspensions.
- Taxon
- AppleMalus domesticat0
- Treatment
- CF of GYUN-1190e0
- Outcome
- pathogen spore germinatione0
- Direction
- inhibitede0
- Qualifier
- at 10 8 cfu/mle0
- Comparator
- cell suspensionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The CF of GYUN-1190 inhibited pathogen spore germination more than cell suspensions at 10 8 cfu/ml.”
t0“Apple”
Claim · model-written summary
GYUN-1190 filtrate suppressed apple fruit bitter rot more than cell suspensions and pyraclostrobin.
- Taxon
- AppleMalus domesticat0
- Treatment
- GYUN-1190e0
- Outcome
- apple fruit bitter rote0
- Direction
- suppressese0
- Comparator
- GYUN-1190 cell suspensions and pyraclostrobine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it suppresses apple fruit bitter rot more effectively than GYUN-1190 cell suspensions and pyraclostrobin in planta.”
t0“Apple”
Claim · model-written summary
Pathogen mycelial growth was completely inhibited after 48 h immersion in culture filtrate.
- Taxon
- AppleMalus domesticat0
- Treatment
- CFe0
- Interactor
- C. fructicolaColletotrichum fructicolae0
- Outcome
- mycelial growth of C. fructicolae0
- Direction
- completely inhibitede0
- Qualifier
- 48 he0
- Comparator
- positive controls and GYUN-1190 cell suspensionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The mycelial growth of C. fructicola was completely inhibited 48 h after immersion into the CF, in compared with positive controls and GYUN-1190 cell suspensions.”
t0“Apple”
The same questions in other papers
pathogen product · disease · Malus domestica (apple)Positive and negative results
4 papers in the same direction as this paper · 3 in the opposite direction
- negative
- negative
- negative
4 papers more in this group
See all 8 papers →pathogen product · Colletotrichum fructicola · Malus domestica (apple)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.