Abstract
Abstract Non‐host resistance (NHR) governs defense responses against a broad range of potential pathogen species in contrast with host resistance. To identify specific genes involved in disease resistance, we used a virus‐induced gene‐silencing screen in Nicotiana benthamiana and identified glycosyltransferase ( NbGT ) as an essential component of NHR. NbGT silencing enhanced the hypersensitivity response, reactive oxygen species response, and callose deposition in N. benthamiana , improving its NHR to Pseudomonas syringae pv. tomato ( Pst ) DC3000. NbGT participated in reactive oxygen species accumulation caused by flg22 rather than coronatine and HrcC of Pst DC3000. Analyses of gene expression and enzyme activity demonstrated that NbGT ‐silenced plants exhibited enhanced expression and elevated levels of superoxide dismutase, resulting in heightened accumulation of H 2 O 2 . In conclusion, NbGT‐silencing increases H 2 O 2 accumulation by regulating superoxide dismutase activity during the immune response to flg22, enhancing resistance to Pst DC3000 in N. benthamiana . This research provides novel insights into the role of glycosyltransferases in NHR.
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Claims 9
Claim · model-written summary
A gene-silencing screen identified NbGT as a component of non-host resistance.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Gene
- NbGTe0
- Outcome
- essential component of NHRe0
- Comparator
- virus‐induced gene‐silencing screene0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we used a virus‐induced gene‐silencing screen in Nicotiana benthamiana and identified glycosyltransferase ( NbGT ) as an essential component of NHR.”
Claim · model-written summary
NbGT silencing enhanced the hypersensitivity response.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- NbGT silencinge0
- Gene
- NbGTe0
- Outcome
- hypersensitivity responsee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NbGT silencing enhanced the hypersensitivity response, reactive oxygen species response, and callose deposition in N. benthamiana”
Claim · model-written summary
NbGT silencing enhanced reactive oxygen species response.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- NbGT silencinge0
- Gene
- NbGTe0
- Outcome
- reactive oxygen species responsee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NbGT silencing enhanced the hypersensitivity response, reactive oxygen species response, and callose deposition in N. benthamiana”
Claim · model-written summary
NbGT silencing enhanced callose deposition.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- NbGT silencinge0
- Gene
- NbGTe0
- Outcome
- callose depositione0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NbGT silencing enhanced the hypersensitivity response, reactive oxygen species response, and callose deposition in N. benthamiana”
Claim · model-written summary
NbGT silencing improved non-host resistance to Pseudomonas syringae pv. tomato DC3000.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- NbGT silencinge0
- Interactor
- Pseudomonas syringae pv. tomato ( Pst ) DC3000
Pseudomonas syringae pv. tomatoe0pathogen - Outcome
- NHRe0
- Direction
- improvinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NbGT silencing enhanced the hypersensitivity response, reactive oxygen species response, and callose deposition in N. benthamiana , improving its NHR to Pseudomonas syringae pv. tomato ( Pst ) DC3000.”
t0“Nicotiana benthamiana”
Claim · model-written summary
NbGT participated in flg22-triggered reactive oxygen species accumulation.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- flg22e0
- Gene
- NbGTe0
- Outcome
- reactive oxygen species accumulatione0
- Comparator
- coronatine and HrcCe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NbGT participated in reactive oxygen species accumulation caused by flg22 rather than coronatine and HrcC of Pst DC3000.”
t0“Nicotiana benthamiana”
Claim · model-written summary
NbGT-silenced plants had enhanced superoxide dismutase expression.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- NbGT ‐silencede0
- Gene
- superoxide dismutasee0
- Outcome
- enhanced expressione0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NbGT ‐silenced plants exhibited enhanced expression and elevated levels of superoxide dismutase”
t0“Nicotiana benthamiana”
Claim · model-written summary
NbGT-silenced plants had elevated superoxide dismutase levels.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- NbGT ‐silencede0
- Gene
- superoxide dismutasee0
- Outcome
- levels of superoxide dismutasee0
- Direction
- elevatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NbGT ‐silenced plants exhibited enhanced expression and elevated levels of superoxide dismutase”
t0“Nicotiana benthamiana”
Claim · model-written summary
NbGT silencing increased hydrogen peroxide accumulation through superoxide dismutase regulation.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Gene
- NbGTe0
- Outcome
- H 2 O 2 accumulatione0
- Direction
- increasese0
- Qualifier
- regulating superoxide dismutase activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NbGT‐silencing increases H 2 O 2 accumulation by regulating superoxide dismutase activity during the immune response to flg22”
t0“Nicotiana benthamiana”
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- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.