Abstract
In order to explore the lipidomic remodeling mechanisms and biological basis of hemp response to cold stress, the physiological and biochemical indices of hemp were analyzed, and a regulatory network for lipid metabolism under cold stress (4 ℃) was constructed. The results revealed that cold stress, inhibited the growth and development of hemp, with the total fresh weight decreasing by 29∼37 %, and the total dry weight decreasing by 17∼22 %. Additionally, the photosynthesis system was damaged, prompting plants to produce osmotic regulators and activate protective enzymes. Lipidomic analysis revealed that galactolipids, including MGDG, DGDG, and SQDG, were the dominant membrane lipids in hemp leaves, accounting for 70 % of the total membrane lipid components. Notably, some lipid remodeling was observed in hemp leaf tissue 3 d after cold stress treatment. Specifically, higher PC/PE and DGDG/MGDG ratios, along with a 53 % increase in PA levels, highlighted the critical role of membrane lipid remodeling and fatty acid unsaturation in hemp adaptation to cold stress. The transcriptomic analysis revealed that the lipid metabolism pathways were activated, with 732 genes associated with lipid metabolic pathways were identified. These genes were primarily enriched in the pathways related to TAG synthesis, fatty acid metabolism, and membrane lipid metabolism. A novel metabolic regulatory network for hemp lipids under cold stress was constructed, offering some insights into the molecular events underlying changes in membrane lipids during cold stress and identifies the key lipids and pathways responsible for resilience to such stress, thereby facilitating the development of new approaches for crop tolerance to stress. • The molecular regulatory network of major lipid metabolism of hemp under cold stress was constructed. • Hemp is a typical 18:3 plant. • MGDG and DGDG of hemp are mainly derived from ER eukaryotic pathway. • Lipid remodeling has an important role in the cold response process of hemp.
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Claims 11
Claim · model-written summary
Cold stress decreased the total fresh weight of hemp by 29∼37 %.
- Taxon
- hemp
Cannabis sativa subsp. sativae1 - Treatment
- cold stresse1
- Outcome
- total fresh weighte1
- Direction
- decreasinge1
- Value
- 29∼37 %e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“cold stress, inhibited the growth and development of hemp, with the total fresh weight decreasing by 29∼37 %, and the total dry weight decreasing by 17∼22 %”
Claim · model-written summary
Cold stress decreased the total dry weight of hemp by 17∼22 %.
- Taxon
- hempCannabis sativa subsp. sativae1
- Treatment
- cold stresse1
- Outcome
- total dry weighte1
- Direction
- decreasinge1
- Value
- 17∼22 %e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“cold stress, inhibited the growth and development of hemp, with the total fresh weight decreasing by 29∼37 %, and the total dry weight decreasing by 17∼22 %”
Claim · model-written summary
Cold stress damaged the photosynthesis system of hemp.
- Taxon
- hempCannabis sativa subsp. sativae1
- Treatment
- cold stresse1
- Outcome
- photosynthesis systeme2
- Direction
- damagede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“cold stress, inhibited the growth and development of hemp, with the total fresh weight decreasing by 29∼37 %, and the total dry weight decreasing by 17∼22 %”
e2“Additionally, the photosynthesis system was damaged, prompting plants to produce osmotic regulators and activate protective enzymes”
Claim · model-written summary
Cold stress increased osmotic regulators in hemp.
- Taxon
- hempCannabis sativa subsp. sativae1
- Treatment
- cold stresse1
- Outcome
- osmotic regulatorse2
- Direction
- increaseinferrede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“cold stress, inhibited the growth and development of hemp, with the total fresh weight decreasing by 29∼37 %, and the total dry weight decreasing by 17∼22 %”
e2“Additionally, the photosynthesis system was damaged, prompting plants to produce osmotic regulators and activate protective enzymes”
Claim · model-written summary
Cold stress increased protective enzymes in hemp.
- Taxon
- hempCannabis sativa subsp. sativae1
- Treatment
- cold stresse1
- Outcome
- protective enzymese2
- Direction
- increaseinferrede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“cold stress, inhibited the growth and development of hemp, with the total fresh weight decreasing by 29∼37 %, and the total dry weight decreasing by 17∼22 %”
e2“Additionally, the photosynthesis system was damaged, prompting plants to produce osmotic regulators and activate protective enzymes”
Claim · model-written summary
Some lipid remodeling was observed in hemp leaf tissue 3 days after cold stress treatment.
- Taxon
- hempCannabis sativa subsp. sativae1
- Organ / stage
- hemp leaf tissuee3
- Outcome
- lipid remodelinge3
- Qualifier
- 3 d after cold stress treatmente3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“cold stress, inhibited the growth and development of hemp, with the total fresh weight decreasing by 29∼37 %, and the total dry weight decreasing by 17∼22 %”
e3“Notably, some lipid remodeling was observed in hemp leaf tissue 3 d after cold stress treatment”
Claim · model-written summary
Cold stress was associated with higher PC/PE and DGDG/MGDG ratios in hemp.
- Taxon
- hempCannabis sativa subsp. sativae1
- Treatment
- cold stresse5
- Outcome
- PC/PE and DGDG/MGDG ratiose5
- Direction
- highere5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“cold stress, inhibited the growth and development of hemp, with the total fresh weight decreasing by 29∼37 %, and the total dry weight decreasing by 17∼22 %”
e5“Specifically, higher PC/PE and DGDG/MGDG ratios, along with a 53 % increase in PA levels, highlighted the critical role of membrane lipid remodeling and fatty acid unsaturation in hemp adaptation to cold stress”
Claim · model-written summary
Cold stress increased PA levels in hemp by 53 %.
- Taxon
- hempCannabis sativa subsp. sativae1
- Treatment
- cold stresse5
- Outcome
- PA levelse5
- Direction
- increasee5
- Value
- 53 %e5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“cold stress, inhibited the growth and development of hemp, with the total fresh weight decreasing by 29∼37 %, and the total dry weight decreasing by 17∼22 %”
e5“Specifically, higher PC/PE and DGDG/MGDG ratios, along with a 53 % increase in PA levels, highlighted the critical role of membrane lipid remodeling and fatty acid unsaturation in hemp adaptation to cold stress”
Claim · model-written summary
Lipid metabolism pathways were activated in hemp under cold stress according to transcriptome analysis.
- Taxon
- hempCannabis sativa subsp. sativae1
- Outcome
- lipid metabolism pathwayse6
- Direction
- activatede6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“cold stress, inhibited the growth and development of hemp, with the total fresh weight decreasing by 29∼37 %, and the total dry weight decreasing by 17∼22 %”
e6“The transcriptomic analysis revealed that the lipid metabolism pathways were activated, with 732 genes associated with lipid metabolic pathways were identified”
Claim · model-written summary
Transcriptome analysis identified 732 genes associated with lipid metabolic pathways in hemp.
- Taxon
- hempCannabis sativa subsp. sativae1
- Outcome
- genes associated with lipid metabolic pathwayse6
- Value
- 732 genese6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“cold stress, inhibited the growth and development of hemp, with the total fresh weight decreasing by 29∼37 %, and the total dry weight decreasing by 17∼22 %”
e6“The transcriptomic analysis revealed that the lipid metabolism pathways were activated, with 732 genes associated with lipid metabolic pathways were identified”
Claim · model-written summary
The lipid-related genes of hemp were primarily enriched in pathways for TAG synthesis, fatty acid metabolism and membrane lipid metabolism.
- Taxon
- hempCannabis sativa subsp. sativae1
- Outcome
- pathways related to TAG synthesis, fatty acid metabolism, and membrane lipid metabolisme7
- Qualifier
- primarily enrichede7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“cold stress, inhibited the growth and development of hemp, with the total fresh weight decreasing by 29∼37 %, and the total dry weight decreasing by 17∼22 %”
e7“These genes were primarily enriched in the pathways related to TAG synthesis, fatty acid metabolism, and membrane lipid metabolism”
The same questions in other papers
cold · growth · Cannabis sativaPositive and negative results
3 in the opposite direction
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.