Abstract
Low-nitrogen (LN) conditions typically promote anthocyanin biosynthesis in plants. Although previous studies primarily explored structural genes and transcription factors involved in anthocyanin biosynthesis, the role of long noncoding RNAs (lncRNAs) in this process under LN conditions remains less understood. This study identified LNC159c as a potential regulator of anthocyanin biosynthesis, with its expression notably downregulated under LN conditions. LNC159c functions as the host gene of miR159c, which regulates its expression. A dual-luciferase reporter assay and GFP signal detection demonstrated that miR159c inhibited MsMYB10 expression through base complementary pairing. Yeast one-hybrid (Y1H) and dual-LUC reporter assays jointly verified that MsMYB10 binds to the F3'H promoter. Stable overexpression in apple calli and transient overexpression in apple fruit peels and M. spectabilis leaves showed that LNC159c overexpression promoted miR159c expression, while inhibiting MsMYB10 expression and anthocyanin biosynthesis. MsMYB10 overexpression promoted anthocyanin biosynthesis, whereas the opposite results were observed in MsMYB10-silenced tissues. Thus, under LN conditions, reduced LNC159c expression limits miR159c production, relieving MsMYB10 inhibition and enhancing anthocyanin accumulation. These findings highlight the regulatory role of lncRNAs in anthocyanin biosynthesis under LN conditions.
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Claims 10
Claim · model-written summary
Low nitrogen downregulated LNC159c expression.
- Taxon
- Malus spectabilis
Malus spectabilist0 - Treatment
- LN conditionse0
- Gene
- LNC159ct0
- Outcome
- expressione0
- Direction
- downregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“its expression notably downregulated under LN conditions”
t0“LNC159c Negatively Regulates Anthocyanin Biosynthesis via miR159c in Malus spectabilis Under Low Nitrogen”
t1“LNC159c Negatively Regulates Anthocyanin Biosynthesis via miR159c in Malus spectabilis Under Low Nitrogen”
Claim · model-written summary
LNC159c functions as the host gene of miR159c.
- Taxon
- Malus spectabilisMalus spectabilist0
- Gene
- LNC159ce0
- Outcome
- host gene of miR159ce0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LNC159c functions as the host gene of miR159c”
t0“LNC159c Negatively Regulates Anthocyanin Biosynthesis via miR159c in Malus spectabilis Under Low Nitrogen”
t1“LNC159c Negatively Regulates Anthocyanin Biosynthesis via miR159c in Malus spectabilis Under Low Nitrogen”
Claim · model-written summary
miR159c inhibited MsMYB10 expression through complementary pairing.
- Taxon
- Malus spectabilisMalus spectabilist0
- Gene
- miR159ce0
- Outcome
- MsMYB10 expressione0
- Direction
- inhibitede0
- Qualifier
- base complementary pairinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“miR159c inhibited MsMYB10 expression through base complementary pairing”
t0“LNC159c Negatively Regulates Anthocyanin Biosynthesis via miR159c in Malus spectabilis Under Low Nitrogen”
t1“LNC159c Negatively Regulates Anthocyanin Biosynthesis via miR159c in Malus spectabilis Under Low Nitrogen”
Claim · model-written summary
MsMYB10 binds to the F3'H promoter.
- Taxon
- Malus spectabilisMalus spectabilist0
- Gene
- MsMYB10e0
- Outcome
- binds to the F3'H promotere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MsMYB10 binds to the F3'H promoter”
t0“LNC159c Negatively Regulates Anthocyanin Biosynthesis via miR159c in Malus spectabilis Under Low Nitrogen”
t1“LNC159c Negatively Regulates Anthocyanin Biosynthesis via miR159c in Malus spectabilis Under Low Nitrogen”
Claim · model-written summary
LNC159c overexpression promoted miR159c expression.
- Taxon
- Malus spectabilisMalus spectabilist0
- Treatment
- LNC159c overexpressione0
- Gene
- miR159ce0
- Outcome
- miR159c expressione0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LNC159c overexpression promoted miR159c expression”
t0“LNC159c Negatively Regulates Anthocyanin Biosynthesis via miR159c in Malus spectabilis Under Low Nitrogen”
t1“LNC159c Negatively Regulates Anthocyanin Biosynthesis via miR159c in Malus spectabilis Under Low Nitrogen”
Claim · model-written summary
LNC159c overexpression inhibited MsMYB10 expression.
- Taxon
- Malus spectabilisMalus spectabilist0
- Treatment
- LNC159c overexpressione0
- Outcome
- MsMYB10 expressione0
- Direction
- inhibitinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LNC159c overexpression promoted miR159c expression, while inhibiting MsMYB10 expression”
t0“LNC159c Negatively Regulates Anthocyanin Biosynthesis via miR159c in Malus spectabilis Under Low Nitrogen”
t1“LNC159c Negatively Regulates Anthocyanin Biosynthesis via miR159c in Malus spectabilis Under Low Nitrogen”
Claim · model-written summary
LNC159c overexpression inhibited anthocyanin biosynthesis.
- Taxon
- Malus spectabilisMalus spectabilist0
- Treatment
- LNC159c overexpressione0
- Outcome
- anthocyanin biosynthesise0
- Direction
- inhibitinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LNC159c overexpression promoted miR159c expression, while inhibiting MsMYB10 expression and anthocyanin biosynthesis”
t0“LNC159c Negatively Regulates Anthocyanin Biosynthesis via miR159c in Malus spectabilis Under Low Nitrogen”
t1“LNC159c Negatively Regulates Anthocyanin Biosynthesis via miR159c in Malus spectabilis Under Low Nitrogen”
Claim · model-written summary
MsMYB10 overexpression promoted anthocyanin biosynthesis.
- Taxon
- Malus spectabilisMalus spectabilist0
- Treatment
- MsMYB10 overexpressione0
- Outcome
- anthocyanin biosynthesise0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MsMYB10 overexpression promoted anthocyanin biosynthesis”
t0“LNC159c Negatively Regulates Anthocyanin Biosynthesis via miR159c in Malus spectabilis Under Low Nitrogen”
t1“LNC159c Negatively Regulates Anthocyanin Biosynthesis via miR159c in Malus spectabilis Under Low Nitrogen”
Claim · model-written summary
Silencing MsMYB10 produced the opposite anthocyanin biosynthesis result.
- Taxon
- Malus spectabilisMalus spectabilist0
- Treatment
- MsMYB10-silenced tissuese0
- Outcome
- anthocyanin biosynthesise0
- Comparator
- MsMYB10 overexpressione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MsMYB10 overexpression promoted anthocyanin biosynthesis, whereas the opposite results were observed in MsMYB10-silenced tissues”
t0“LNC159c Negatively Regulates Anthocyanin Biosynthesis via miR159c in Malus spectabilis Under Low Nitrogen”
t1“LNC159c Negatively Regulates Anthocyanin Biosynthesis via miR159c in Malus spectabilis Under Low Nitrogen”
Claim · model-written summary
Under low nitrogen, reduced LNC159c expression limits miR159c production and relieves MsMYB10 inhibition, enhancing anthocyanin accumulation.
- Taxon
- Malus spectabilisMalus spectabilist0
- Gene
- LNC159ce0
- Outcome
- anthocyanin accumulatione0
- Direction
- enhancinge0
- Qualifier
- reduced LNC159c expression limits miR159c productione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“under LN conditions, reduced LNC159c expression limits miR159c production, relieving MsMYB10 inhibition and enhancing anthocyanin accumulation”
t0“LNC159c Negatively Regulates Anthocyanin Biosynthesis via miR159c in Malus spectabilis Under Low Nitrogen”
t1“LNC159c Negatively Regulates Anthocyanin Biosynthesis via miR159c in Malus spectabilis Under Low Nitrogen”