Abstract
BACKGROUND: Persistent lead contamination and the absence of natural remediation elements exacerbate the long-term toxicity of plants. Nevertheless, it has been consistently shown that selenium has a protective effect against heavy metal toxicity in plants. Consequently, it is imperative to identify the metabolic pathways that selenium employs to enhance the resistance of plants to lead stress. This study aimed to investigate the metabolomic alterations induced by selenium priming of Vicia faba seeds to enhance their tolerance to lead stress. RESULTS: Selenium seed priming significantly improved the growth parameter and mitigated the adverse growth consequences observed under lead stress. Nuclear magnetic resonance-based metabolomic analysis identified 58 metabolites in the polar extracts of the shoots, with the metabolites composed of amino acids (40%), carboxylic acids (12%), fatty acids (11%), carbohydrates (5%), alkaloids (5%), and phenols (4%). The addition of Pb facilitated the biosynthesis of unique metabolites, including 2-methylglutarate, 3-methyladipate, and epinephrine, which were absent in control and selenium-treated samples. Conversely, 4-aminobutyrate and 2-methylglutarate were entirely absent in Pb samples. Selenium-treated plants accumulated trigonelline and AMP at levels 1.4 and 6.0 times, respectively, more than the control samples. Selenium-primed plants exposed to lead stress exhibited higher levels of asparagine, tryptophan, and xanthine compared to other treatments. As determined by both enrichment analysis and pathway analysis, the most significantly altered pathways were alanine, aspartate, and glutamate metabolism; aminoacyl-tRNA biosynthesis; and valine, leucine, and isoleucine biosynthesis pathways. CONCLUSION: The results demonstrate the crucial role of selenium priming in enhancing the growth and lead stress resistance of Vicia faba plants by significantly altering the concentrations of key metabolites and metabolic pathways, particularly those involved in amino acid metabolism, offering a promising strategy for improving plant resilience to heavy metal contamination.
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Claims 16
Claim · model-written summary
Selenium seed priming significantly improved growth under lead stress.
- Taxon
- Vicia faba
Vicia fabae1 - Treatment
- Selenium seed priminge0
- Outcome
- growth parametere0
- Direction
- improvede0
- Qualifier
- under lead stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Selenium seed priming significantly improved the growth parameter and mitigated the adverse growth consequences observed under lead stress”
e1“Vicia faba seeds”
Claim · model-written summary
NMR metabolomics identified 58 shoot metabolites.
- Taxon
- Vicia fabaVicia fabae1
- Organ / stage
- shootse0
- Outcome
- metabolitese0
- Value
- 58e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nuclear magnetic resonance-based metabolomic analysis identified 58 metabolites in the polar extracts of the shoots”
e1“Vicia faba seeds”
Claim · model-written summary
The identified metabolites included amino acids at 40%.
- Taxon
- Vicia fabaVicia fabae1
- Outcome
- amino acidse0
- Value
- 40%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“metabolites composed of amino acids (40%), carboxylic acids (12%), fatty acids (11%), carbohydrates (5%), alkaloids (5%), and phenols (4%)”
e1“Vicia faba seeds”
Claim · model-written summary
The identified metabolites included carboxylic acids at 12%.
- Taxon
- Vicia fabaVicia fabae1
- Outcome
- carboxylic acidse0
- Value
- 12%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“metabolites composed of amino acids (40%), carboxylic acids (12%), fatty acids (11%), carbohydrates (5%), alkaloids (5%), and phenols (4%)”
e1“Vicia faba seeds”
Claim · model-written summary
The identified metabolites included fatty acids at 11%.
- Taxon
- Vicia fabaVicia fabae1
- Outcome
- fatty acidse0
- Value
- 11%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“metabolites composed of amino acids (40%), carboxylic acids (12%), fatty acids (11%), carbohydrates (5%), alkaloids (5%), and phenols (4%)”
e1“Vicia faba seeds”
Claim · model-written summary
The identified metabolites included carbohydrates at 5%.
- Taxon
- Vicia fabaVicia fabae1
- Outcome
- carbohydratese0
- Value
- 5%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“metabolites composed of amino acids (40%), carboxylic acids (12%), fatty acids (11%), carbohydrates (5%), alkaloids (5%), and phenols (4%)”
e1“Vicia faba seeds”
Claim · model-written summary
The identified metabolites included alkaloids at 5%.
- Taxon
- Vicia fabaVicia fabae1
- Outcome
- alkaloidse0
- Value
- 5%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“metabolites composed of amino acids (40%), carboxylic acids (12%), fatty acids (11%), carbohydrates (5%), alkaloids (5%), and phenols (4%)”
e1“Vicia faba seeds”
Claim · model-written summary
The identified metabolites included phenols at 4%.
- Taxon
- Vicia fabaVicia fabae1
- Outcome
- phenolse0
- Value
- 4%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“metabolites composed of amino acids (40%), carboxylic acids (12%), fatty acids (11%), carbohydrates (5%), alkaloids (5%), and phenols (4%)”
e1“Vicia faba seeds”
Claim · model-written summary
Lead promoted unique 2-methylglutarate, 3-methyladipate and epinephrine absent from control and selenium samples.
- Taxon
- Vicia fabaVicia fabae1
- Treatment
- addition of Pbe0
- Outcome
- 2-methylglutarate, 3-methyladipate, and epinephrinee0
- Comparator
- control and selenium-treated samplese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The addition of Pb facilitated the biosynthesis of unique metabolites, including 2-methylglutarate, 3-methyladipate, and epinephrine, which were absent in control and selenium-treated samples”
e1“Vicia faba seeds”
Claim · model-written summary
4-aminobutyrate and 2-methylglutarate were absent in Pb samples.
- Taxon
- Vicia fabaVicia fabae1
- Outcome
- 4-aminobutyrate and 2-methylglutarate were entirely absente0
- Qualifier
- Pb samplese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“4-aminobutyrate and 2-methylglutarate were entirely absent in Pb samples”
e1“Vicia faba seeds”
Claim · model-written summary
Selenium-treated plants had 1.4-fold control trigonelline.
- Taxon
- Vicia fabaVicia fabae1
- Treatment
- Selenium-treatede0
- Outcome
- trigonellinee0
- Direction
- moree0
- Value
- 1.4e0
- Comparator
- control samplese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Selenium-treated plants accumulated trigonelline and AMP at levels 1.4 and 6.0 times, respectively, more than the control samples”
e1“Vicia faba seeds”
Claim · model-written summary
Selenium-treated plants had 6.0-fold control AMP.
- Taxon
- Vicia fabaVicia fabae1
- Treatment
- Selenium-treatede0
- Outcome
- AMPe0
- Direction
- moree0
- Value
- 6.0 timese0
- Comparator
- control samplese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Selenium-treated plants accumulated trigonelline and AMP at levels 1.4 and 6.0 times, respectively, more than the control samples”
e1“Vicia faba seeds”
Claim · model-written summary
Selenium-primed plants under lead stress had higher asparagine, tryptophan and xanthine than other treatments.
- Taxon
- Vicia fabaVicia fabae1
- Treatment
- Selenium-primed plants exposed to lead stresse0
- Outcome
- levels of asparagine, tryptophan, and xanthinee0
- Direction
- highere0
- Comparator
- other treatmentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Selenium-primed plants exposed to lead stress exhibited higher levels of asparagine, tryptophan, and xanthine compared to other treatments”
e1“Vicia faba seeds”
Claim · model-written summary
Alanine, aspartate and glutamate metabolism was among the most altered pathways.
- Taxon
- Vicia fabaVicia fabae1
- Outcome
- alanine, aspartate, and glutamate metabolisme0
- Direction
- alterede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the most significantly altered pathways were alanine, aspartate, and glutamate metabolism; aminoacyl-tRNA biosynthesis; and valine, leucine, and isoleucine biosynthesis pathways”
e1“Vicia faba seeds”
Claim · model-written summary
Aminoacyl-tRNA biosynthesis was among the most altered pathways.
- Taxon
- Vicia fabaVicia fabae1
- Outcome
- aminoacyl-tRNA biosynthesise0
- Direction
- alterede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the most significantly altered pathways were alanine, aspartate, and glutamate metabolism; aminoacyl-tRNA biosynthesis; and valine, leucine, and isoleucine biosynthesis pathways”
e1“Vicia faba seeds”
Claim · model-written summary
Valine, leucine and isoleucine biosynthesis was among the most altered pathways.
- Taxon
- Vicia fabaVicia fabae1
- Outcome
- valine, leucine, and isoleucine biosynthesis pathwayse0
- Direction
- alterede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the most significantly altered pathways were alanine, aspartate, and glutamate metabolism; aminoacyl-tRNA biosynthesis; and valine, leucine, and isoleucine biosynthesis pathways”
e1“Vicia faba seeds”
The same questions in other papers
selenium atom · growth · Vicia faba (faba bean)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
heavy metal · molecular: metabolites · Vicia faba (faba bean)Positive and negative results
2 in the opposite direction
- negative
- negative
seed treatment · growth · Vicia faba (faba bean)Positive and negative results
13 papers in the same direction as this paper · 3 in the opposite direction
- positive
- negative
- no effect
13 papers more in this group
See all 17 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.