Abstract
Anthocyanins are the main pigments in ripe strawberry fruits. FaMYB10 and abscisic acid (ABA) are the two major regulators of anthocyanin biosynthesis in the maturation process of strawberry fruits. However, the transcriptional regulation of FaMYB10 , ABA biosynthesis, and anthocyanin accumulation in strawberry ( Fragaria × ananassa ) remain largely unknown. Here, a yeast one-hybrid screen using the FaMYB10 promoter identified a class B MADS-domain transcription factor, FaMADS6 in strawberry. FaMADS6 exhibited high expression at the early developmental stage but was hardly detected during maturation of strawberry fruit, a pattern opposite to accumulation of anthocyanins. Transcriptional upregulation of FaMADS6 repressed anthocyanin accumulation and expression of FaMYB10 and the anthocyanin biosynthetic genes, FaCHS , FaCHI , FaF3H , FaANS , and FaUFGT . In contrast, downregulation of FaMADS6 promoted the expression of FaMYB10 and the anthocyanin biosynthetic genes. The promoters of the anthocyanin biosynthetic genes were not directly bound by FaMADS6, in contrast to FaMYB10 . Analysis of the DNA binding sequences of FaMADS6 revealed that it also interacted with the promoters of FaNCED2 and FaPYR1 , which are involved in the biosynthesis and perception of ABA. Overexpression of FaMADS6 significantly suppressed FaNCED2 and FaPYR1 and ABA synthesis in transgenic strawberry. Together, our findings suggest that FaMADS6 functions as a suppressor of anthocyanin accumulation by directly downregulating FaMYB10 and ABA production during strawberry fruit maturation.
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Claims 11
Claim · model-written summary
A yeast one-hybrid screen identified FaMADS6 as a class B MADS transcription factor interacting with the FaMYB10 promoter.
- Taxon
- strawberry
_single2.jpg?width=60)
Fragaria ×ananassae0 - Gene
- FaMADS6e0
- Outcome
- identified a class B MADS-domain transcription factore0
- Qualifier
- using the FaMYB10 promotere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“a yeast one-hybrid screen using the FaMYB10 promoter identified a class B MADS-domain transcription factor, FaMADS6 in strawberry”
Claim · model-written summary
FaMADS6 expression was high early and barely detectable during strawberry fruit maturation.
- Taxon
- strawberryFragaria ×ananassae0
- Organ / stage
- fruite0
- Gene
- FaMADS6e0
- Outcome
- FaMADS6 exhibited high expression at the early developmental stage but was hardly detected during maturatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FaMADS6 exhibited high expression at the early developmental stage but was hardly detected during maturation of strawberry fruit”
Claim · model-written summary
FaMADS6 expression had a pattern opposite to anthocyanin accumulation.
- Taxon
- strawberryFragaria ×ananassae1
- Gene
- FaMADS6e1
- Outcome
- accumulation of anthocyaninse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a pattern opposite to accumulation of anthocyanins”
e1“FaMADS6 exhibited high expression at the early developmental stage but was hardly detected during maturation of strawberry fruit”
Claim · model-written summary
Upregulating FaMADS6 repressed anthocyanin accumulation.
- Taxon
- strawberryFragaria ×ananassat0
- Treatment
- Transcriptional upregulation of FaMADS6e0
- Gene
- FaMADS6e0
- Outcome
- anthocyanin accumulatione0
- Direction
- repressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcriptional upregulation of FaMADS6 repressed anthocyanin accumulation”
t0“strawberry”
Claim · model-written summary
Upregulating FaMADS6 repressed FaMYB10 and anthocyanin biosynthetic gene expression.
- Taxon
- strawberryFragaria ×ananassat0
- Treatment
- Transcriptional upregulation of FaMADS6e0
- Gene
- FaMADS6e0
- Outcome
- expression of FaMYB10 and the anthocyanin biosynthetic genese0
- Direction
- repressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcriptional upregulation of FaMADS6 repressed anthocyanin accumulation and expression of FaMYB10 and the anthocyanin biosynthetic genes, FaCHS , FaCHI , FaF3H , FaANS , and FaUFGT”
t0“strawberry”
Claim · model-written summary
Downregulating FaMADS6 promoted FaMYB10 and anthocyanin biosynthetic gene expression.
- Taxon
- strawberryFragaria ×ananassat0
- Treatment
- downregulation of FaMADS6e0
- Gene
- FaMADS6e0
- Outcome
- expression of FaMYB10 and the anthocyanin biosynthetic genese0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“downregulation of FaMADS6 promoted the expression of FaMYB10 and the anthocyanin biosynthetic genes”
t0“strawberry”
Claim · model-written summary
FaMADS6 bound the FaMYB10 promoter but not promoters of anthocyanin biosynthetic genes.
- Taxon
- strawberryFragaria ×ananassat0
- Gene
- FaMADS6e0
- Outcome
- promoters of the anthocyanin biosynthetic genes were not directly bounde0
- Qualifier
- in contrast to FaMYB10e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The promoters of the anthocyanin biosynthetic genes were not directly bound by FaMADS6, in contrast to FaMYB10”
t0“strawberry”
Claim · model-written summary
FaMADS6 interacted with promoters of FaNCED2 and FaPYR1.
- Taxon
- strawberryFragaria ×ananassat0
- Gene
- FaMADS6e1
- Outcome
- interacted with the promoters of FaNCED2 and FaPYR1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it also interacted with the promoters of FaNCED2 and FaPYR1”
e1“Analysis of the DNA binding sequences of FaMADS6”
t0“strawberry”
Claim · model-written summary
FaMADS6 overexpression suppressed FaNCED2 and FaPYR1 in transgenic strawberry.
- Taxon
- strawberryFragaria ×ananassae0
- Treatment
- Overexpression of FaMADS6e0
- Gene
- FaMADS6e0
- Outcome
- FaNCED2 and FaPYR1e0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of FaMADS6 significantly suppressed FaNCED2 and FaPYR1 and ABA synthesis in transgenic strawberry”
Claim · model-written summary
FaMADS6 overexpression suppressed ABA synthesis in transgenic strawberry.
- Taxon
- strawberryFragaria ×ananassae0
- Treatment
- Overexpression of FaMADS6e0
- Gene
- FaMADS6e0
- Outcome
- ABA synthesise0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of FaMADS6 significantly suppressed FaNCED2 and FaPYR1 and ABA synthesis in transgenic strawberry”
Claim · model-written summary
The authors infer FaMADS6 suppresses anthocyanin accumulation by directly downregulating FaMYB10 and ABA production.
- Taxon
- strawberryFragaria ×ananassae0
- Gene
- FaMADS6e0
- Outcome
- anthocyanin accumulatione0
- Qualifier
- by directly downregulating FaMYB10 and ABA productione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FaMADS6 functions as a suppressor of anthocyanin accumulation by directly downregulating FaMYB10 and ABA production during strawberry fruit maturation”
The same questions in other papers
loss of function · molecular: expression · Fragaria x ananassa (strawberry)Positive and negative results
1 paper in the same direction as this paper · 3 in the opposite direction
- no effect
- negative
- negative
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.