Paper · 2025 · Plant Protection

Comparative efficacy of Zn-EDTA and ZnSO4 in mitigating zinc deficiency in wheat for sustainable production

Muhammad Asif Siddique; Saleem Maseeh Bhatti; Inayatullah Rajpar; et al.

17
claims
31
verbatim quotes
0
citations
2
research areas
✓
Open access

Abstract

Zinc (Zn) deficiency in soils and crops poses a significant challenge to sustainable wheat production and human nutrition worldwide. Agronomic biofortification, enhancing cereal crops with zinc, is a rapid and effective strategy to address this issue. The present field study evaluated two zinc sources, zinc sulfate (ZnSO4·H2O, 33% Zn) and chelated zinc (Zn-EDTA, 5% Zn), for their effectiveness in enhancing yield and Zn concentration in Zn-responsive wheat varieties (Anmol-91, TD-1, NIA-Sarang, and NIA-Amber). The Zn treatments included: a control (no Zn), 13.2 kg Zn ha-1 as ZnSO4·H2O, and 0.125 and 0.25 kg Zn ha-1 as Zn-EDTA. Treatments were arranged in a randomized complete block split-plot design with three replications. Zinc application significantly improved wheat growth and yield traits compared to the control. However, no significant interaction was observed between treatments and varieties for agronomic parameters. Application of 0.25 kg Zn ha-1 as Zn-EDTA significantly increased plant height (by 4%), spike length (31%), number of spikelets per spike (7%), 1000-grain weight (12%), straw yield (10%), grain yield (10%), Zn concentration (16%), the phytic acid-to-zinc molar ratio (PA:Zn) (46%), and total absorbable zinc (TAZ) (87%) compared to the control. Furthermore, it significantly reduced phytic acid concentration in wheat grains by 37%. Soil Zn concentration also increased significantly (P 0.05), with the highest increase observed at 13.2 kg ha-1 ZnSO4 (125%) and the lowest with Zn-EDTA (95%) compared to the control. Among the wheat varieties evaluated, Anmol-91, NIA-Amber, and NIA-Sarang demonstrated the best performance.

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Claims 17

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Zinc application significantly improved wheat growth and yield traits versus control.

Treatment
Zinc applicatione0
Outcome
growth and yield traitse0
Direction
improvede0
Comparator
controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Zinc application significantly improved wheat growth and yield traits compared to the control.”

✓ located verbatim in the source · abstract, characters 733–827 · show in text ↑
Associationobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

There was no significant treatment by variety interaction for agronomic parameters.

Treatment
treatmentse0
Outcome
interactione0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“wheat”

✓ located verbatim in the source · title, characters 75–80 · show in text ↑
e0

“no significant interaction was observed between treatments and varieties for agronomic parameters”

✓ located verbatim in the source · abstract, characters 837–934 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Zn-EDTA at 0.25 kg Zn ha-1 increased wheat plant height by 4%.

Treatment
0.25 kg Zn ha-1 as Zn-EDTAe0
Outcome
plant heighte0
Direction
increasede0
Value
4%e0
Comparator
controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“wheat”

✓ located verbatim in the source · title, characters 75–80 · show in text ↑
e0

“Application of 0.25 kg Zn ha-1 as Zn-EDTA significantly increased plant height (by 4%), spike length (31%), number of spikelets per spike (7%), 1000-grain weight (12%), straw yield (10%), grain yield (10%), Zn concentration (16%), the phytic acid-to-zinc molar ratio (PA:Zn) (46%), and total absorbable zinc (TAZ) (87%) compared to the control.”

✓ located verbatim in the source · abstract, characters 936–1280 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Zn-EDTA increased wheat spike length by 31%.

Treatment
0.25 kg Zn ha-1 as Zn-EDTAe0
Outcome
spike lengthe0
Direction
increasede0
Value
31%e0
Comparator
controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“wheat”

✓ located verbatim in the source · title, characters 75–80 · show in text ↑
e0

“Application of 0.25 kg Zn ha-1 as Zn-EDTA significantly increased plant height (by 4%), spike length (31%), number of spikelets per spike (7%), 1000-grain weight (12%), straw yield (10%), grain yield (10%), Zn concentration (16%), the phytic acid-to-zinc molar ratio (PA:Zn) (46%), and total absorbable zinc (TAZ) (87%) compared to the control.”

✓ located verbatim in the source · abstract, characters 936–1280 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Zn-EDTA increased spikelets per spike by 7%.

Treatment
0.25 kg Zn ha-1 as Zn-EDTAe0
Outcome
number of spikelets per spikee0
Direction
increasede0
Value
7%e0
Comparator
controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“wheat”

✓ located verbatim in the source · title, characters 75–80 · show in text ↑
e0

“Application of 0.25 kg Zn ha-1 as Zn-EDTA significantly increased plant height (by 4%), spike length (31%), number of spikelets per spike (7%), 1000-grain weight (12%), straw yield (10%), grain yield (10%), Zn concentration (16%), the phytic acid-to-zinc molar ratio (PA:Zn) (46%), and total absorbable zinc (TAZ) (87%) compared to the control.”

✓ located verbatim in the source · abstract, characters 936–1280 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Zn-EDTA increased thousand grain weight by 12%.

Treatment
0.25 kg Zn ha-1 as Zn-EDTAe0
Outcome
1000-grain weighte0
Direction
increasede0
Value
12%e0
Comparator
controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“wheat”

✓ located verbatim in the source · title, characters 75–80 · show in text ↑
e0

“Application of 0.25 kg Zn ha-1 as Zn-EDTA significantly increased plant height (by 4%), spike length (31%), number of spikelets per spike (7%), 1000-grain weight (12%), straw yield (10%), grain yield (10%), Zn concentration (16%), the phytic acid-to-zinc molar ratio (PA:Zn) (46%), and total absorbable zinc (TAZ) (87%) compared to the control.”

✓ located verbatim in the source · abstract, characters 936–1280 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Zn-EDTA increased straw yield by 10%.

Treatment
0.25 kg Zn ha-1 as Zn-EDTAe0
Outcome
straw yielde0
Direction
increasede0
Value
10%e0
Comparator
controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“wheat”

✓ located verbatim in the source · title, characters 75–80 · show in text ↑
e0

“Application of 0.25 kg Zn ha-1 as Zn-EDTA significantly increased plant height (by 4%), spike length (31%), number of spikelets per spike (7%), 1000-grain weight (12%), straw yield (10%), grain yield (10%), Zn concentration (16%), the phytic acid-to-zinc molar ratio (PA:Zn) (46%), and total absorbable zinc (TAZ) (87%) compared to the control.”

✓ located verbatim in the source · abstract, characters 936–1280 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Zn-EDTA increased grain yield by 10%.

Treatment
0.25 kg Zn ha-1 as Zn-EDTAe0
Outcome
grain yielde0
Direction
increasede0
Value
10%e0
Comparator
controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“wheat”

✓ located verbatim in the source · title, characters 75–80 · show in text ↑
e0

“Application of 0.25 kg Zn ha-1 as Zn-EDTA significantly increased plant height (by 4%), spike length (31%), number of spikelets per spike (7%), 1000-grain weight (12%), straw yield (10%), grain yield (10%), Zn concentration (16%), the phytic acid-to-zinc molar ratio (PA:Zn) (46%), and total absorbable zinc (TAZ) (87%) compared to the control.”

✓ located verbatim in the source · abstract, characters 936–1280 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Zn-EDTA increased zinc concentration by 16%.

Treatment
0.25 kg Zn ha-1 as Zn-EDTAe0
Outcome
Zn concentratione0
Direction
increasede0
Value
16%e0
Comparator
controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“wheat”

✓ located verbatim in the source · title, characters 75–80 · show in text ↑
e0

“Application of 0.25 kg Zn ha-1 as Zn-EDTA significantly increased plant height (by 4%), spike length (31%), number of spikelets per spike (7%), 1000-grain weight (12%), straw yield (10%), grain yield (10%), Zn concentration (16%), the phytic acid-to-zinc molar ratio (PA:Zn) (46%), and total absorbable zinc (TAZ) (87%) compared to the control.”

✓ located verbatim in the source · abstract, characters 936–1280 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Zn-EDTA increased the phytic acid to zinc molar ratio by 46%.

Treatment
0.25 kg Zn ha-1 as Zn-EDTAe0
Outcome
phytic acid-to-zinc molar ratio (PA:Zn)e0
Direction
increasede0
Value
46%e0
Comparator
controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“wheat”

✓ located verbatim in the source · title, characters 75–80 · show in text ↑
e0

“Application of 0.25 kg Zn ha-1 as Zn-EDTA significantly increased plant height (by 4%), spike length (31%), number of spikelets per spike (7%), 1000-grain weight (12%), straw yield (10%), grain yield (10%), Zn concentration (16%), the phytic acid-to-zinc molar ratio (PA:Zn) (46%), and total absorbable zinc (TAZ) (87%) compared to the control.”

✓ located verbatim in the source · abstract, characters 936–1280 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Zn-EDTA increased total absorbable zinc by 87%.

Treatment
0.25 kg Zn ha-1 as Zn-EDTAe0
Outcome
total absorbable zinc (TAZ)e0
Direction
increasede0
Value
87%e0
Comparator
controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“wheat”

✓ located verbatim in the source · title, characters 75–80 · show in text ↑
e0

“Application of 0.25 kg Zn ha-1 as Zn-EDTA significantly increased plant height (by 4%), spike length (31%), number of spikelets per spike (7%), 1000-grain weight (12%), straw yield (10%), grain yield (10%), Zn concentration (16%), the phytic acid-to-zinc molar ratio (PA:Zn) (46%), and total absorbable zinc (TAZ) (87%) compared to the control.”

✓ located verbatim in the source · abstract, characters 936–1280 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Zn-EDTA reduced grain phytic acid concentration by 37%.

Treatment
0.25 kg Zn ha-1 as Zn-EDTAe1
Outcome
phytic acid concentratione0
Direction
reducede0
Value
37%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“wheat”

✓ located verbatim in the source · title, characters 75–80 · show in text ↑
e0

“it significantly reduced phytic acid concentration in wheat grains by 37%”

✓ located verbatim in the source · abstract, characters 1294–1367 · show in text ↑
e1

“Application of 0.25 kg Zn ha-1 as Zn-EDTA”

✓ located verbatim in the source · abstract, characters 936–977 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Zinc sulfate increased soil zinc concentration by 125% versus control.

Treatment
13.2 kg ha-1 ZnSO4e0
Outcome
Soil Zn concentratione0
Direction
increasede0
Value
125%e0
Comparator
controle0

Not reportedTaxon

Verbatim

t0

“wheat”

✓ located verbatim in the source · title, characters 75–80 · show in text ↑
e0

“Soil Zn concentration also increased significantly (P 0.05), with the highest increase observed at 13.2 kg ha-1 ZnSO4 (125%) and the lowest with Zn-EDTA (95%) compared to the control.”

✓ located verbatim in the source · abstract, characters 1369–1552 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Zn-EDTA increased soil zinc concentration by 95% versus control.

Treatment
Zn-EDTAe0
Outcome
Soil Zn concentratione0
Direction
increasede0
Value
95%e0
Comparator
controle0

Not reportedTaxon

Verbatim

t0

“wheat”

✓ located verbatim in the source · title, characters 75–80 · show in text ↑
e0

“Soil Zn concentration also increased significantly (P 0.05), with the highest increase observed at 13.2 kg ha-1 ZnSO4 (125%) and the lowest with Zn-EDTA (95%) compared to the control.”

✓ located verbatim in the source · abstract, characters 1369–1552 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Anmol-91 was among the best performing wheat varieties.

Material
Anmol-91e0
Outcome
best performancee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Among the wheat varieties evaluated, Anmol-91, NIA-Amber, and NIA-Sarang demonstrated the best performance.”

✓ located verbatim in the source · abstract, characters 1553–1660 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

NIA-Amber was among the best performing wheat varieties.

Material
NIA-Ambere0
Outcome
best performancee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Among the wheat varieties evaluated, Anmol-91, NIA-Amber, and NIA-Sarang demonstrated the best performance.”

✓ located verbatim in the source · abstract, characters 1553–1660 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

NIA-Sarang was among the best performing wheat varieties.

Material
NIA-Sarange0
Outcome
best performancee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Among the wheat varieties evaluated, Anmol-91, NIA-Amber, and NIA-Sarang demonstrated the best performance.”

✓ located verbatim in the source · abstract, characters 1553–1660 · show in text ↑

zinc atom · molecular: metabolites · Triticum aestivum (wheat)Results differ

111 papers: 85 positive, 26 negative or no effect

84 papers in the same direction as this paper · 25 in the opposite direction · 1 reporting no difference from an alternative

  1. negative
  2. negative
  3. negative

107 papers more in this group

See all 111 papers →

zinc atom · growth · Triticum aestivum (wheat)Results differ

200 papers: 155 positive, 45 negative or no effect

154 papers in the same direction as this paper · 43 in the opposite direction · 2 reporting no difference from an alternative

  1. no effect
  2. no effect
    Wheat plants with low Mn or low Zn supply showed no growth differences from controls at the three harvests.

    Distribution and remobilization of Zn and Mn during grain development in wheat · 1994 · compared with controls (sufficient Zn and Mn)

    Opposite directionAbout lacking this nutrient, so it counts the other way round

  3. negative

196 papers more in this group

See all 200 papers →

zinc atom · nutrient: content · Triticum aestivum (wheat)Results differ

380 papers: 347 positive, 33 negative or no effect

346 papers in the same direction as this paper · 33 in the opposite direction

  1. negative
  2. negative
  3. no effect

376 papers more in this group

See all 380 papers →

zinc atom · yield · Triticum aestivum (wheat)Results differ

511 papers: 456 positive, 55 negative or no effect

455 papers in the same direction as this paper · 49 in the opposite direction · 6 reporting no difference from an alternative

  1. no effect
  2. negative
  3. no effect

507 papers more in this group

See all 511 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.