Abstract
Saline-alkali stress inhibited the normal growth and development of plants, which seriously restricted the yield of crops. Maize is one of the most important crops in the world. However, the mechanism of maize in response to saline-alkali stress is still largely unknown. Through the observation of growth parameters and the detection of physiological and biochemical indicators in saline-alkali tolerant (22KN3894) and saline-alkali sensitive (H23146) maize inbred lines, this study found that compared with H23146, 22KN3894 accumulated less ROS content and more total flavonoids content, while the degree of root damage and ion toxicity was relatively small. Full-length transcriptome and broadly targeted metabolome were used to analyse the response mechanism of extreme maize inbred lines to saline-alkali stress. 22KN3894 accumulated more metabolites such as sugars and flavonoids. There were significant differences in the contents of flavonoid metabolites and genes related to flavonoid synthesis between the two materials. Weighted gene co-expression network analysis and co-expression network analysis based on RNA-Seq data suggested that the ZmWRKY82 gene might respond to saline-alkali stress by regulating the flavonoid biosynthesis pathway. ZmWRKY82 directly bound to the W-box in the ZmCHI6 promoter and promoted its expression. The above results showed that ZmWRKY82 could improve the antioxidant capacity by promoting the transcription of ZmCHI6 and the synthesis of flavonoids, thereby resisting saline-alkali stress. These findings provided novel insights for improving maize saline-alkali stress tolerance, demonstrating that flavonoids played pivotal roles in plant stress adaptation, and laid the foundation for future mechanistic studies and breeding improvement.
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Claims 10
Claim · model-written summary
Under saline-alkali stress, the tolerant line 22KN3894 accumulated less ROS than the sensitive line H23146.
- Material
- 22KN3894e1
- Treatment
- Saline-alkali stresse0
- Outcome
- ROS contente2
- Direction
- lesse2
- Comparator
- H23146e1
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Saline-alkali stress inhibited the normal growth and development of plants”
e1“saline-alkali tolerant (22KN3894) and saline-alkali sensitive (H23146) maize inbred lines”
e2“compared with H23146, 22KN3894 accumulated less ROS content and more total flavonoids content”
Claim · model-written summary
Under saline-alkali stress, the tolerant line 22KN3894 had more total flavonoid content than the sensitive line H23146.
- Material
- 22KN3894e1
- Treatment
- Saline-alkali stresse0
- Outcome
- total flavonoids contente2
- Direction
- moree2
- Comparator
- H23146e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Saline-alkali stress inhibited the normal growth and development of plants”
e1“saline-alkali tolerant (22KN3894) and saline-alkali sensitive (H23146) maize inbred lines”
e2“compared with H23146, 22KN3894 accumulated less ROS content and more total flavonoids content”
Claim · model-written summary
Under saline-alkali stress, the tolerant line 22KN3894 had relatively small root damage and ion toxicity compared with H23146.
- Material
- 22KN3894e1
- Treatment
- Saline-alkali stresse0
- Outcome
- degree of root damage and ion toxicitye3
- Direction
- relatively smalle3
- Comparator
- H23146e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Saline-alkali stress inhibited the normal growth and development of plants”
e1“saline-alkali tolerant (22KN3894) and saline-alkali sensitive (H23146) maize inbred lines”
e3“while the degree of root damage and ion toxicity was relatively small”
Claim · model-written summary
Line 22KN3894 accumulated more metabolites, such as sugars and flavonoids.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“saline-alkali tolerant (22KN3894) and saline-alkali sensitive (H23146) maize inbred lines”
e4“22KN3894 accumulated more metabolites such as sugars and flavonoids”
Claim · model-written summary
Flavonoid metabolite contents and flavonoid synthesis genes differed significantly between the two maize lines.
- Outcome
- contents of flavonoid metabolites and genes related to flavonoid synthesise5
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“saline-alkali tolerant (22KN3894) and saline-alkali sensitive (H23146) maize inbred lines”
e5“There were significant differences in the contents of flavonoid metabolites and genes related to flavonoid synthesis between the two materials”
Claim · model-written summary
ZmWRKY82 might respond to saline-alkali stress by regulating the flavonoid biosynthesis pathway, according to co-expression network analysis.
- Treatment
- saline-alkali stresse6
- Gene
- ZmWRKY82e6
- Outcome
- respond to saline-alkali stresse6
- Qualifier
- by regulating the flavonoid biosynthesis pathwaye6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“saline-alkali tolerant (22KN3894) and saline-alkali sensitive (H23146) maize inbred lines”
e6“Weighted gene co-expression network analysis and co-expression network analysis based on RNA-Seq data suggested that the ZmWRKY82 gene might respond to saline-alkali stress by regulating the flavonoid biosynthesis pathway”
Claim · model-written summary
ZmWRKY82 directly bound to the W-box in the ZmCHI6 promoter.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“saline-alkali tolerant (22KN3894) and saline-alkali sensitive (H23146) maize inbred lines”
e7“ZmWRKY82 directly bound to the W-box in the ZmCHI6 promoter and promoted its expression”
Claim · model-written summary
ZmWRKY82 promoted expression of ZmCHI6.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“saline-alkali tolerant (22KN3894) and saline-alkali sensitive (H23146) maize inbred lines”
e7“ZmWRKY82 directly bound to the W-box in the ZmCHI6 promoter and promoted its expression”
Claim · model-written summary
ZmWRKY82 could improve antioxidant capacity by promoting ZmCHI6 transcription and flavonoid synthesis, thereby resisting saline-alkali stress.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“saline-alkali tolerant (22KN3894) and saline-alkali sensitive (H23146) maize inbred lines”
e7“ZmWRKY82 could improve the antioxidant capacity by promoting the transcription of ZmCHI6 and the synthesis of flavonoids, thereby resisting saline-alkali stress”
Claim · model-written summary
Flavonoids play a pivotal role in stress adaptation of maize.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“saline-alkali tolerant (22KN3894) and saline-alkali sensitive (H23146) maize inbred lines”
e7“demonstrating that flavonoids played pivotal roles in plant stress adaptation”
The same questions in other papers
salinity · pest · Zea mays (maize)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.