Abstract
Alkaline stress, driven by high pH and carbonate accumulation, results in severe physiological damage in plants. While the molecular mechanisms underlying alkaline tolerance have been partially elucidated in many crops, they remain largely unexplored in wheat. We hypothesize that alkaline stress tolerance in wheat is genotype-dependent. This study employed an integrated multi-omics approach to assess alkaline stress responses, combining genome-wide association study (GWAS) and RNA-seq analyses. Systematic phenotyping revealed severe alkaline stress-induced root architecture remodeling-with 57% and 73% length reductions after 1- and 3-day treatments, respectively-across 258 accessions. Analysis of the GWAS results identified nine significant alkaline tolerance QTLs on chromosomes 1A, 3B, 3D, 4A, and 5B, along with 285 associated candidate genes. Using contrasting genotypes-Dingxi 38 (tolerant) and TDP.D-27 (sensitive)-as experimental materials, physiological analyses demonstrated that root elongation was less inhibited in Dingxi 38 under alkaline stress compared to TDP.D-27, with superior root integrity observed in the tolerant genotype. Concurrently, Dingxi 38 exhibited enhanced reactive oxygen species (ROS) scavenging capacity. Subsequent RNA-seq analysis identified differentially expressed genes (DEGs) involved in ion homeostasis, oxidative defense, and cell wall remodeling. Integrated GWAS and RNA-seq analyses allowed for the identification of seven high-confidence candidate genes, including transcription factors (MYB38, bHLH148), metabolic regulators (ATP-PFK3), and transporters (OCT7), elucidating a mechanistic basis for adaptation to alkaline conditions. These findings advance our understanding of alkaline tolerance in wheat and provide candidate targets for molecular breeding of saline- and alkaline-tolerant crops.
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Claims 11
Claim · model-written summary
After 1-day alkaline treatment, root architecture length was reduced by 57%.
- Taxon
- Bread Wheat
Triticum aestivumt1 - Treatment
- alkaline stresse0
- Outcome
- lengthe1
- Direction
- reductionse1
- Value
- 57%e1
- Qualifier
- after 1- and 3-day treatments, respectivelye1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“Bread Wheat (Triticum aestivum L.)”
e0“Systematic phenotyping revealed severe alkaline stress”
e1“with 57% and 73% length reductions after 1- and 3-day treatments, respectively”
Claim · model-written summary
After 3-day alkaline treatment, root architecture length was reduced by 73%.
- Taxon
- Bread WheatTriticum aestivumt1
- Treatment
- alkaline stresse0
- Outcome
- lengthe1
- Direction
- reductionse1
- Value
- 73%e1
- Qualifier
- after 1- and 3-day treatments, respectivelye1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“Bread Wheat (Triticum aestivum L.)”
e0“Systematic phenotyping revealed severe alkaline stress”
e1“with 57% and 73% length reductions after 1- and 3-day treatments, respectively”
Claim · model-written summary
The GWAS identified nine significant alkaline tolerance QTLs on chromosomes 1A, 3B, 3D, 4A and 5B.
- Taxon
- Bread WheatTriticum aestivumt1
- Gene
- alkaline tolerance QTLse2
- Outcome
- significant alkaline tolerance QTLse2
- Value
- ninee2
- Qualifier
- on chromosomes 1A, 3B, 3D, 4A, and 5Be2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“Bread Wheat (Triticum aestivum L.)”
e2“Analysis of the GWAS results identified nine significant alkaline tolerance QTLs on chromosomes 1A, 3B, 3D, 4A, and 5B, along with 285 associated candidate genes”
Claim · model-written summary
The GWAS associated 285 candidate genes with alkaline tolerance.
- Taxon
- Bread WheatTriticum aestivumt1
- Gene
- alkaline tolerance QTLse2
- Outcome
- associated candidate genese2
- Value
- 285e2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“Bread Wheat (Triticum aestivum L.)”
e2“Analysis of the GWAS results identified nine significant alkaline tolerance QTLs on chromosomes 1A, 3B, 3D, 4A, and 5B, along with 285 associated candidate genes”
Claim · model-written summary
Root elongation of Dingxi 38 was less inhibited than that of TDP.D-27 under alkaline stress.
- Taxon
- Bread WheatTriticum aestivumt1
- Material
- Dingxi 38e3
- Treatment
- alkaline stresse3
- Outcome
- root elongatione3
- Direction
- less inhibitede3
- Comparator
- TDP.D-27e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“Bread Wheat (Triticum aestivum L.)”
e3“root elongation was less inhibited in Dingxi 38 under alkaline stress compared to TDP.D-27”
Claim · model-written summary
The tolerant genotype showed superior root integrity under alkaline stress.
- Taxon
- Bread WheatTriticum aestivumt1
- Material
- tolerant genotypee4
- Outcome
- root integritye4
- Qualifier
- superiore4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“Bread Wheat (Triticum aestivum L.)”
e4“with superior root integrity observed in the tolerant genotype”
Claim · model-written summary
Dingxi 38 exhibited enhanced reactive oxygen species (ROS) scavenging capacity.
- Taxon
- Bread WheatTriticum aestivumt1
- Material
- Dingxi 38e5
- Outcome
- reactive oxygen species (ROS) scavenging capacitye5
- Direction
- enhancede5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“Bread Wheat (Triticum aestivum L.)”
e5“Dingxi 38 exhibited enhanced reactive oxygen species (ROS) scavenging capacity”
Claim · model-written summary
RNA-seq identified differentially expressed genes involved in ion homeostasis, oxidative defense and cell wall remodeling.
- Taxon
- Bread WheatTriticum aestivumt1
- Outcome
- differentially expressed genes (DEGs)e6
- Qualifier
- involved in ion homeostasis, oxidative defense, and cell wall remodelinge6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“Bread Wheat (Triticum aestivum L.)”
e6“differentially expressed genes (DEGs) involved in ion homeostasis, oxidative defense, and cell wall remodeling”
Claim · model-written summary
Integrated GWAS and RNA-seq analyses identified seven high-confidence candidate genes, including MYB38 and bHLH148.
- Taxon
- Bread WheatTriticum aestivumt1
- Gene
- MYB38, bHLH148e7
- Outcome
- high-confidence candidate genese7
- Value
- sevene7
- Qualifier
- metabolic regulators (ATP-PFK3), and transporters (OCT7)e7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“Bread Wheat (Triticum aestivum L.)”
e7“Integrated GWAS and RNA-seq analyses allowed for the identification of seven high-confidence candidate genes, including transcription factors (MYB38, bHLH148), metabolic regulators (ATP-PFK3), and transporters (OCT7)”
Claim · model-written summary
The integrated analysis elucidates a mechanistic basis for wheat adaptation to alkaline conditions.
- Taxon
- Bread WheatTriticum aestivumt1
- Outcome
- adaptation to alkaline conditionse2
- Qualifier
- elucidating a mechanistic basise2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“Bread Wheat (Triticum aestivum L.)”
e2“elucidating a mechanistic basis for adaptation to alkaline conditions”
Claim · model-written summary
The authors suggest the candidate genes as targets for molecular breeding of alkaline-tolerant wheat.
- Taxon
- Bread WheatTriticum aestivumt1
- Outcome
- candidate targets for molecular breedinge2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“Bread Wheat (Triticum aestivum L.)”
e2“provide candidate targets for molecular breeding”
The same questions in other papers
alkalinity · growth · Triticum aestivum (wheat)Positive and negative results
4 papers in the same direction as this paper · 11 in the opposite direction
- negative
- negative
- negative
12 papers more in this group
See all 16 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.