Abstract
• Potassium sulfate treatment can increase the sucrose content of strawberry fruits. • FaSWEET17 negatively regulates sucrose transport in strawberry fruits. • FaSWEET17 regulates strawberry sucrose content by modulating sugar metabolism genes. In plants, the sugar content in fruit is a key factor in determining flavor quality and market competitiveness. SWEET family sugar transport proteins are involved in transmembrane sugar transport, but their roles in sugar translocation within strawberry fruits remain relatively understudied. Given that potassium fertilization is often reported to regulate fruit sugar metabolism, this study first investigated the effects of potassium sulfate treatment on sugar accumulation in strawberry fruits. It was found that potassium sulfate treatment not only increased the sucrose content in 'Yanli' strawberry fruits, but also down-regulated the expression level of the FaSWEET17 . Spatiotemporal expression analysis revealed that FaSWEET17 expression was highest in floral organs and immature green fruit stages, and was significantly down-regulated during fruit ripening. Subcellular localization assays confirmed that the FaSWEET17 protein is localized to the plasma membrane. Functional validation experiments demonstrated that overexpression of FaSWEET17 significantly reduced sucrose content in the fruits of both 'Yanli' and 'Yuehua' strawberry cultivars. Conversely, silencing this gene markedly promoted sucrose accumulation. RT-qPCR analysis further revealed that genetic manipulation of FaSWEET17 significantly affected the expression of key genes involved in sucrose synthesis and metabolic pathways. In conclusion, this study provides the first evidence that strawberry SWEET17 acts as a negative regulator of fruit sucrose content. It down-regulation represents a vital mechanism promoting sucrose accumulation in fruits, providing novel theoretical foundations and a candidate target for molecular breeding approaches aimed at the targeted regulation of strawberry fruit sugar metabolism and quality improvement.
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Claims 10
Claim · model-written summary
Potassium sulfate increased sucrose in Yanli strawberry fruit.
- Taxon
- strawberry
_single2.jpg?width=60)
Fragaria ×ananassae0 - Material
- 'Yanli'e0
- Organ / stage
- fruitse0
- Treatment
- potassium sulfate treatmente0
- Outcome
- sucrose contente0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“potassium sulfate treatment not only increased the sucrose content in 'Yanli' strawberry fruits”
Claim · model-written summary
Potassium sulfate downregulated FaSWEET17 expression.
- Taxon
- strawberryFragaria ×ananassae0
- Material
- 'Yanli'e0
- Treatment
- potassium sulfate treatmente0
- Gene
- FaSWEET17e0
- Outcome
- expression level of the FaSWEET17e0
- Direction
- down-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“potassium sulfate treatment not only increased the sucrose content in 'Yanli' strawberry fruits, but also down-regulated the expression level of the FaSWEET17”
Claim · model-written summary
FaSWEET17 expression was highest in floral organs and immature green fruit.
- Taxon
- strawberryFragaria ×ananassat0
- Organ / stage
- floral organs and immature green fruit stagese0
- Gene
- FaSWEET17e0
- Outcome
- FaSWEET17 expressione0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FaSWEET17 expression was highest in floral organs and immature green fruit stages”
t0“strawberry”
Claim · model-written summary
FaSWEET17 expression fell significantly during ripening.
- Taxon
- strawberryFragaria ×ananassat0
- Organ / stage
- fruit ripeninge0
- Gene
- FaSWEET17e0
- Outcome
- FaSWEET17 expressione0
- Direction
- down-regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FaSWEET17 expression was highest in floral organs and immature green fruit stages, and was significantly down-regulated during fruit ripening”
t0“strawberry”
Claim · model-written summary
FaSWEET17 protein localized to the plasma membrane.
- Taxon
- strawberryFragaria ×ananassat0
- Gene
- FaSWEET17 proteine0
- Outcome
- localized to the plasma membranee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the FaSWEET17 protein is localized to the plasma membrane”
t0“strawberry”
Claim · model-written summary
FaSWEET17 overexpression significantly reduced sucrose in Yanli fruit.
- Taxon
- strawberryFragaria ×ananassae0
- Material
- 'Yanli'e0
- Organ / stage
- fruitse0
- Treatment
- overexpression of FaSWEET17e0
- Gene
- FaSWEET17e0
- Outcome
- sucrose contente0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of FaSWEET17 significantly reduced sucrose content in the fruits of both 'Yanli' and 'Yuehua' strawberry cultivars”
Claim · model-written summary
FaSWEET17 overexpression significantly reduced sucrose in Yuehua fruit.
- Taxon
- strawberryFragaria ×ananassae0
- Material
- 'Yuehua'e0
- Organ / stage
- fruitse0
- Treatment
- overexpression of FaSWEET17e0
- Gene
- FaSWEET17e0
- Outcome
- sucrose contente0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of FaSWEET17 significantly reduced sucrose content in the fruits of both 'Yanli' and 'Yuehua' strawberry cultivars”
Claim · model-written summary
Silencing FaSWEET17 promoted sucrose accumulation.
- Taxon
- strawberryFragaria ×ananassat0
- Treatment
- silencing this genee0
- Gene
- FaSWEET17t0
- Outcome
- sucrose accumulatione0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing this gene markedly promoted sucrose accumulation”
t0“FaSWEET17 negative regulates sucrose accumulation in strawberry fruit”
Claim · model-written summary
FaSWEET17 manipulation significantly changed expression of sucrose metabolism genes.
- Taxon
- strawberryFragaria ×ananassat0
- Treatment
- genetic manipulation of FaSWEET17e0
- Gene
- FaSWEET17e0
- Outcome
- expression of key genes involved in sucrose synthesis and metabolic pathwayse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“genetic manipulation of FaSWEET17 significantly affected the expression of key genes involved in sucrose synthesis and metabolic pathways”
t0“strawberry”
Claim · model-written summary
Strawberry SWEET17 was interpreted as a negative regulator of fruit sucrose.
- Taxon
- strawberryFragaria ×ananassae0
- Gene
- SWEET17e0
- Outcome
- fruit sucrose contente0
- Direction
- negative regulatore0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“strawberry SWEET17 acts as a negative regulator of fruit sucrose content”
The same questions in other papers
potassium sulfate · quality: sugar starch · Fragaria x ananassa (strawberry)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.