Abstract
Introduction Low-temperature stress can cause damage to the growth and development of tobacco plants and the yield and quality of tobacco leaves. Methods To elucidate the physiological and molecular mechanisms underlying the differing responses of different tobacco varieties to low-temperature stress at 6 °C, transcriptomics analysis was employed to investigate the differences in physiological and gene regulatory networks between K326 and CV-1. Results Morphological analysis revealed that CV-1 recovered more quickly to its pre-cold stress state than K326 during the later stages of low-temperature stress, demonstrating stronger cold tolerance. Physiological and biochemical analyses showed that compared to K326, CV-1 exhibited stronger antioxidant enzyme activities (superoxide dismutase, catalase, peroxidase) and lower membrane lipid peroxidation damage, as indicated by the decreased malondialdehyde content. Differential gene expression analysis indicated that the enhanced cold tolerance of CV-1 may be attributed to stronger phenylalanine synthesis capacity, NADH synthesis, and antioxidant enzyme activities. Weighted co-expression network analysis revealed that the enhanced cold tolerance of CV-1 may be attributed to its unique SUMOylation and phosphorylation regulatory pathways of proteins such as DeSI1L , EDS1L , and EXPA2L . Compared to K326 under low-temperature stress, CV-1 also exhibits stronger photosynthetic capacity, hydrogen peroxide transport capacity, and isoflavone synthesis capacity. Additionally, CV-1 shows higher expression of the SPFH protein superfamily and heat shock protein family. Discussion This study revealed differences in gene regulatory networks between K326 and CV-1 in response to low-temperature stress and identified candidate genes associated with low-temperature stress, which can be utilized for genetic improvement of tobacco plants to enhance their cold tolerance.
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Claims 9
Claim · model-written summary
CV-1 recovered more quickly than K326 to its pre-cold stress state in the later stages of low-temperature stress
- Taxon
- tobacco
Nicotiana tabacume0 - Material
- CV-1e2
- Treatment
- low-temperature stresse2
- Outcome
- pre-cold stress statee2
- Direction
- more quicklye2
- Comparator
- K326e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Low-temperature stress can cause damage to the growth and development of tobacco plants”
e2“Morphological analysis revealed that CV-1 recovered more quickly to its pre-cold stress state than K326 during the later stages of low-temperature stress, demonstrating stronger cold tolerance”
Claim · model-written summary
CV-1 had stronger antioxidant enzyme activities (superoxide dismutase, catalase, peroxidase) than K326 under low-temperature stress
- Taxon
- tobaccoNicotiana tabacume0
- Material
- CV-1e3
- Treatment
- low-temperature stress at 6 °Ce1
- Outcome
- antioxidant enzyme activitiese3
- Direction
- strongere3
- Qualifier
- superoxide dismutase, catalase, peroxidasee3
- Comparator
- K326e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Low-temperature stress can cause damage to the growth and development of tobacco plants”
e1“low-temperature stress at 6 °C”
e3“Physiological and biochemical analyses showed that compared to K326, CV-1 exhibited stronger antioxidant enzyme activities (superoxide dismutase, catalase, peroxidase) and lower membrane lipid peroxidation damage, as indicated by the decreased malondialdehyde content”
Claim · model-written summary
CV-1 had lower membrane lipid peroxidation damage than K326 under low-temperature stress, indicated by decreased malondialdehyde content
- Taxon
- tobaccoNicotiana tabacume0
- Material
- CV-1e3
- Treatment
- low-temperature stress at 6 °Ce1
- Outcome
- membrane lipid peroxidation damagee3
- Direction
- lowere3
- Qualifier
- decreased malondialdehyde contente3
- Comparator
- K326e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Low-temperature stress can cause damage to the growth and development of tobacco plants”
e1“low-temperature stress at 6 °C”
e3“Physiological and biochemical analyses showed that compared to K326, CV-1 exhibited stronger antioxidant enzyme activities (superoxide dismutase, catalase, peroxidase) and lower membrane lipid peroxidation damage, as indicated by the decreased malondialdehyde content”
Claim · model-written summary
Enhanced cold tolerance of CV-1 may be attributed to stronger phenylalanine synthesis capacity, NADH synthesis and antioxidant enzyme activities
- Taxon
- tobaccoNicotiana tabacume0
- Compound
- phenylalaninee4
- Outcome
- enhanced cold tolerancee4
- Qualifier
- stronger phenylalanine synthesis capacity, NADH synthesis, and antioxidant enzyme activitiese4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Low-temperature stress can cause damage to the growth and development of tobacco plants”
e4“Differential gene expression analysis indicated that the enhanced cold tolerance of CV-1 may be attributed to stronger phenylalanine synthesis capacity, NADH synthesis, and antioxidant enzyme activities”
Claim · model-written summary
Enhanced cold tolerance of CV-1 may be attributed to its unique SUMOylation and phosphorylation regulatory pathways of DeSI1L, EDS1L and EXPA2L
- Taxon
- tobaccoNicotiana tabacume0
- Gene
- DeSI1L , EDS1L , and EXPA2Le5
- Outcome
- enhanced cold tolerancee5
- Qualifier
- unique SUMOylation and phosphorylation regulatory pathwayse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Low-temperature stress can cause damage to the growth and development of tobacco plants”
e5“Weighted co-expression network analysis revealed that the enhanced cold tolerance of CV-1 may be attributed to its unique SUMOylation and phosphorylation regulatory pathways of proteins such as DeSI1L , EDS1L , and EXPA2L”
Claim · model-written summary
Compared to K326 under low-temperature stress, CV-1 had stronger photosynthetic capacity, hydrogen peroxide transport capacity and isoflavone synthesis capacity
- Taxon
- tobaccoNicotiana tabacume0
- Material
- CV-1e6
- Treatment
- low-temperature stresse6
- Outcome
- photosynthetic capacity, hydrogen peroxide transport capacity, and isoflavone synthesis capacitye6
- Direction
- strongere6
- Comparator
- K326e6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Low-temperature stress can cause damage to the growth and development of tobacco plants”
e6“Compared to K326 under low-temperature stress, CV-1 also exhibits stronger photosynthetic capacity, hydrogen peroxide transport capacity, and isoflavone synthesis capacity”
Claim · model-written summary
CV-1 shows higher expression of the SPFH protein superfamily and heat shock protein family than K326
- Taxon
- tobaccoNicotiana tabacume0
- Material
- CV-1e7
- Gene
- SPFH protein superfamilye7
- Outcome
- expressione7
- Direction
- highere7
- Qualifier
- heat shock protein familye7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Low-temperature stress can cause damage to the growth and development of tobacco plants”
e7“Additionally, CV-1 shows higher expression of the SPFH protein superfamily and heat shock protein family”
Claim · model-written summary
Candidate genes associated with low-temperature stress were identified in tobacco
- Taxon
- tobaccoNicotiana tabacume0
- Outcome
- candidate genese7
- Qualifier
- associated with low-temperature stresse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Low-temperature stress can cause damage to the growth and development of tobacco plants”
e7“identified candidate genes associated with low-temperature stress”
Claim · model-written summary
The candidate genes can be utilized for genetic improvement of tobacco to enhance cold tolerance
- Taxon
- tobaccoNicotiana tabacume0
- Outcome
- genetic improvemente6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Low-temperature stress can cause damage to the growth and development of tobacco plants”
e6“can be utilized for genetic improvement of tobacco plants to enhance their cold tolerance”
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