Abstract
Plants frequently encounter repeated cold stress throughout their life cycle. When subjected to future environmental stimulations, plants stress response can be activated after experiencing priming events during the seed stage. This study employed multi omics approaches to investigate the intricate response mechanisms to cold stress at five true leaves and one central bud in two tobacco varieties, resistant variety NC102 and sensitive variety PVH06, which underwent cold- (SC) and non-priming (CK) at the seed stage. After cold stress, NC102 in SC exhibited the strongest tolerance capacity, with relative water content 1.06 times that of CK. Superoxide dismutase and catalase activities, along with proline content, increased by 25 %, 42 %, and 50 %, respectively. Additionally, hydrogen peroxide and malondialdehyde decreased by 23 % and 12 %, respectively. Both the tricarboxylic acid cycle and amino acid metabolism in NC102 seed priming (NS) and PVH06 seed priming (PS) reacted positive responses to cold stress, with NS accumulating higher levels of unsaturated fatty acids and jasmonic acid. The co-expression network identified three hub genes TAR3 , ABP19A , and psb28 that were enriched in PS and associated with auxin regulation. PS might enhance cold tolerance by upregulating the early auxin responsive genes GH3 and SAUR . Additionally, genes and intermediate metabolites involved in the lignin biosynthetic pathway were significantly expressed in both NS and PS, with a more pronounced response observed in NS. Key metabolites of the lignin biosynthetic pathway were regulated to increase in response to cold stress, and the expression levels of structural genes associated with this pathway were detected via qRT-PCR. This work advances understanding of cold stress tolerance, aiding breeding research. • Cold priming during seed stage enhanced plant resistance when exposed to stress. • Metabolites in TCA cycle and amino acid metabolic were increased for stress response. • Auxin early response genes were upregulated in PS to enhance cold tolerance. • Lignin biosynthesis genes in NS were upregulated enhancing content and resistance.
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Claims 15
Claim · model-written summary
After cold stress, NC102 seed priming gave 1.06 times the relative water content of the non-primed control.
- Taxon
- tobacco
Nicotiana tabacume0 - Material
- NC102e0
- Treatment
- SCe1
- Outcome
- relative water contente1
- Direction
- higherinferrede1
- Value
- 1.06 timese1
- Comparator
- CKe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two tobacco varieties, resistant variety NC102 and sensitive variety PVH06”
e1“After cold stress, NC102 in SC exhibited the strongest tolerance capacity, with relative water content 1.06 times that of CK”
Claim · model-written summary
After cold stress, superoxide dismutase activity increased by 25 % in tobacco under seed cold priming.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- cold stresse1
- Outcome
- Superoxide dismutasee2
- Direction
- increasede2
- Value
- 25 %e2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two tobacco varieties, resistant variety NC102 and sensitive variety PVH06”
e1“After cold stress, NC102 in SC exhibited the strongest tolerance capacity, with relative water content 1.06 times that of CK”
e2“Superoxide dismutase and catalase activities, along with proline content, increased by 25 %, 42 %, and 50 %, respectively”
Claim · model-written summary
After cold stress, catalase activity increased by 42 % in tobacco under seed cold priming.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- cold stresse1
- Outcome
- catalase activitiese2
- Direction
- increasede2
- Value
- 42 %e2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two tobacco varieties, resistant variety NC102 and sensitive variety PVH06”
e1“After cold stress, NC102 in SC exhibited the strongest tolerance capacity, with relative water content 1.06 times that of CK”
e2“Superoxide dismutase and catalase activities, along with proline content, increased by 25 %, 42 %, and 50 %, respectively”
Claim · model-written summary
After cold stress, proline content increased by 50 % in tobacco under seed cold priming.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- cold stresse1
- Outcome
- proline contente2
- Direction
- increasede2
- Value
- 50 %e2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two tobacco varieties, resistant variety NC102 and sensitive variety PVH06”
e1“After cold stress, NC102 in SC exhibited the strongest tolerance capacity, with relative water content 1.06 times that of CK”
e2“Superoxide dismutase and catalase activities, along with proline content, increased by 25 %, 42 %, and 50 %, respectively”
Claim · model-written summary
After cold stress, hydrogen peroxide decreased by 23 % in tobacco under seed cold priming.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- cold stresse1
- Outcome
- hydrogen peroxidee3
- Direction
- decreasede3
- Value
- 23 %e3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two tobacco varieties, resistant variety NC102 and sensitive variety PVH06”
e1“After cold stress, NC102 in SC exhibited the strongest tolerance capacity, with relative water content 1.06 times that of CK”
e3“hydrogen peroxide and malondialdehyde decreased by 23 % and 12 %, respectively”
Claim · model-written summary
After cold stress, malondialdehyde decreased by 12 % in tobacco under seed cold priming.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- cold stresse1
- Outcome
- malondialdehydee3
- Direction
- decreasede3
- Value
- 12 %e3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two tobacco varieties, resistant variety NC102 and sensitive variety PVH06”
e1“After cold stress, NC102 in SC exhibited the strongest tolerance capacity, with relative water content 1.06 times that of CK”
e3“hydrogen peroxide and malondialdehyde decreased by 23 % and 12 %, respectively”
Claim · model-written summary
NC102 seed priming showed positive responses of the tricarboxylic acid cycle and amino acid metabolism to cold stress.
- Taxon
- tobaccoNicotiana tabacume0
- Material
- NC102e4
- Treatment
- cold stresse4
- Outcome
- tricarboxylic acid cycle and amino acid metabolisme4
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two tobacco varieties, resistant variety NC102 and sensitive variety PVH06”
e4“Both the tricarboxylic acid cycle and amino acid metabolism in NC102 seed priming (NS) and PVH06 seed priming (PS) reacted positive responses to cold stress”
Claim · model-written summary
PVH06 seed priming showed positive responses of the tricarboxylic acid cycle and amino acid metabolism to cold stress.
- Taxon
- tobaccoNicotiana tabacume0
- Material
- PVH06e4
- Treatment
- cold stresse4
- Outcome
- tricarboxylic acid cycle and amino acid metabolisme4
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two tobacco varieties, resistant variety NC102 and sensitive variety PVH06”
e4“Both the tricarboxylic acid cycle and amino acid metabolism in NC102 seed priming (NS) and PVH06 seed priming (PS) reacted positive responses to cold stress”
Claim · model-written summary
NS accumulated higher levels of unsaturated fatty acids and jasmonic acid under cold stress.
- Taxon
- tobaccoNicotiana tabacume0
- Material
- NSe5
- Treatment
- cold stresse1
- Outcome
- unsaturated fatty acids and jasmonic acide5
- Direction
- highere5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two tobacco varieties, resistant variety NC102 and sensitive variety PVH06”
e1“After cold stress, NC102 in SC exhibited the strongest tolerance capacity, with relative water content 1.06 times that of CK”
e5“with NS accumulating higher levels of unsaturated fatty acids and jasmonic acid”
Claim · model-written summary
Three hub genes, TAR3, ABP19A and psb28, were enriched in PS and associated with auxin regulation.
- Taxon
- tobaccoNicotiana tabacume0
- Material
- PSe6
- Gene
- TAR3 , ABP19A , and psb28e6
- Outcome
- auxin regulatione6
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two tobacco varieties, resistant variety NC102 and sensitive variety PVH06”
e6“The co-expression network identified three hub genes TAR3 , ABP19A , and psb28 that were enriched in PS and associated with auxin regulation”
Claim · model-written summary
PS might enhance cold tolerance by upregulating the early auxin responsive genes GH3 and SAUR.
- Taxon
- tobaccoNicotiana tabacume0
- Material
- PSe7
- Gene
- GH3 and SAURe7
- Outcome
- cold tolerancee7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two tobacco varieties, resistant variety NC102 and sensitive variety PVH06”
e7“PS might enhance cold tolerance by upregulating the early auxin responsive genes GH3 and SAUR”
Claim · model-written summary
Genes and intermediate metabolites of the lignin biosynthetic pathway were significantly expressed in NS.
- Taxon
- tobaccoNicotiana tabacume0
- Material
- NSe6
- Outcome
- genes and intermediate metabolites involved in the lignin biosynthetic pathwaye6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two tobacco varieties, resistant variety NC102 and sensitive variety PVH06”
e6“genes and intermediate metabolites involved in the lignin biosynthetic pathway were significantly expressed in both NS and PS, with a more pronounced response observed in NS”
Claim · model-written summary
Genes and intermediate metabolites of the lignin biosynthetic pathway were significantly expressed in PS.
- Taxon
- tobaccoNicotiana tabacume0
- Material
- PSe6
- Outcome
- genes and intermediate metabolites involved in the lignin biosynthetic pathwaye6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two tobacco varieties, resistant variety NC102 and sensitive variety PVH06”
e6“genes and intermediate metabolites involved in the lignin biosynthetic pathway were significantly expressed in both NS and PS, with a more pronounced response observed in NS”
Claim · model-written summary
The lignin pathway response was more pronounced in NS than in PS.
- Taxon
- tobaccoNicotiana tabacume0
- Material
- NSe6
- Outcome
- responsee6
- Direction
- more pronouncede6
- Comparator
- PSe6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two tobacco varieties, resistant variety NC102 and sensitive variety PVH06”
e6“genes and intermediate metabolites involved in the lignin biosynthetic pathway were significantly expressed in both NS and PS, with a more pronounced response observed in NS”
Claim · model-written summary
Key metabolites of the lignin biosynthetic pathway were regulated to increase in response to cold stress.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- cold stresse7
- Outcome
- Key metabolites of the lignin biosynthetic pathwaye7
- Direction
- increasee7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two tobacco varieties, resistant variety NC102 and sensitive variety PVH06”
e7“Key metabolites of the lignin biosynthetic pathway were regulated to increase in response to cold stress”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.