Abstract
Jatropha curcas is a promising feedstock for biodiesel and bio-jet fuels production; however, its seed yield is constrained by limited inflorescences. SPL9 is a member of the SBP-box gene family that promotes the juvenile-to-adult phase transition. Accumulating evidence demonstrated that the miR156/SPL module plays important roles in regulating diverse plant developmental processes. Here, we reveal that JcSPL9 regulates both seed yield and oil content in Jatropha. JcSPL9 is highly expressed in fruits and upregulated with age in Jatropha. Overexpression of miR156-resistant JcSPL9 (rJcSPL9) significantly increased seed yield and oil content, whereas overexpression of JcmiR156a had the opposite effects. The highest seed yield in rJcSPL9 transgenic plants was 80.76% greater than that in the WT plants, with a concomitant 12.6% increase in seed oil content. Correspondingly, JcmiR156a transgenic plants displayed 51.67% lower seed yield and 8.28% lower seed oil content compared to WT. Additionally, seed oil fatty acid composition was significantly altered in both rJcSPL9 and JcmiR156a transgenic Jatropha and Arabidopsis, as well as in Arabidopsis spl9 mutants. The key oil biosynthesis genes, including JcWRI1, JcDGAT1, JcDGAT2, and JcOLEOSIN, were upregulated in rJcSPL9 transgenic seeds but downregulated in JcmiR156a transformants. This study provides the first evidence that the miR156/SPL9 module regulates lipid accumulation and fatty acid biosynthesis in seeds. These results highlight SPL9 as a promising target for enhancing oil yield and quality in Jatropha and other oilseed crops.
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Claims 14
Claim · model-written summary
JcSPL9 is highly expressed in fruits of Jatropha
- Taxon
- Jatropha curcas
_tree_at_Samedha.jpg?width=60)
Jatropha curcase0 - Organ / stage
- fruitse1
- Gene
- JcSPL9e1
- Outcome
- highly expressede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_tree_at_Samedha.jpg?width=500)
Verbatim
e0“Jatropha curcas is a promising feedstock for biodiesel and bio-jet fuels production”
e1“JcSPL9 is highly expressed in fruits and upregulated with age in Jatropha”
Claim · model-written summary
JcSPL9 expression is upregulated with age in Jatropha
- Taxon
- Jatropha curcasJatropha curcase0
- Gene
- JcSPL9e1
- Outcome
- upregulatede1
- Qualifier
- with agee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Jatropha curcas is a promising feedstock for biodiesel and bio-jet fuels production”
e1“JcSPL9 is highly expressed in fruits and upregulated with age in Jatropha”
Claim · model-written summary
Overexpression of miR156-resistant JcSPL9 raised seed yield of transgenic Jatropha by 80.76% over wild-type
- Taxon
- Jatropha curcasJatropha curcase0
- Treatment
- Overexpression of miR156-resistant JcSPL9e2
- Outcome
- seed yielde2
- Direction
- greatere3
- Value
- 80.76%e3
- Comparator
- WT plantse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Jatropha curcas is a promising feedstock for biodiesel and bio-jet fuels production”
e2“Overexpression of miR156-resistant JcSPL9 (rJcSPL9) significantly increased seed yield and oil content, whereas overexpression of JcmiR156a had the opposite effects”
e3“The highest seed yield in rJcSPL9 transgenic plants was 80.76% greater than that in the WT plants, with a concomitant 12.6% increase in seed oil content”
Claim · model-written summary
Overexpression of miR156-resistant JcSPL9 raised seed oil content of transgenic Jatropha by 12.6% over wild-type
- Taxon
- Jatropha curcasJatropha curcase0
- Treatment
- Overexpression of miR156-resistant JcSPL9e2
- Outcome
- seed oil contente3
- Direction
- increasee3
- Value
- 12.6%e3
- Comparator
- WT plantse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Jatropha curcas is a promising feedstock for biodiesel and bio-jet fuels production”
e2“Overexpression of miR156-resistant JcSPL9 (rJcSPL9) significantly increased seed yield and oil content, whereas overexpression of JcmiR156a had the opposite effects”
e3“The highest seed yield in rJcSPL9 transgenic plants was 80.76% greater than that in the WT plants, with a concomitant 12.6% increase in seed oil content”
Claim · model-written summary
Overexpression of JcmiR156a lowered seed yield of transgenic Jatropha by 51.67% relative to wild-type
- Taxon
- Jatropha curcasJatropha curcase0
- Treatment
- overexpression of JcmiR156ae2
- Outcome
- seed yielde5
- Direction
- lowere5
- Value
- 51.67%e5
- Comparator
- WTe5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Jatropha curcas is a promising feedstock for biodiesel and bio-jet fuels production”
e2“Overexpression of miR156-resistant JcSPL9 (rJcSPL9) significantly increased seed yield and oil content, whereas overexpression of JcmiR156a had the opposite effects”
e5“Correspondingly, JcmiR156a transgenic plants displayed 51.67% lower seed yield and 8.28% lower seed oil content compared to WT”
Claim · model-written summary
Overexpression of JcmiR156a lowered seed oil content of transgenic Jatropha by 8.28% relative to wild-type
- Taxon
- Jatropha curcasJatropha curcase0
- Treatment
- overexpression of JcmiR156ae2
- Outcome
- seed oil contente5
- Direction
- lowere5
- Value
- 8.28%e5
- Comparator
- WTe5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Jatropha curcas is a promising feedstock for biodiesel and bio-jet fuels production”
e2“Overexpression of miR156-resistant JcSPL9 (rJcSPL9) significantly increased seed yield and oil content, whereas overexpression of JcmiR156a had the opposite effects”
e5“Correspondingly, JcmiR156a transgenic plants displayed 51.67% lower seed yield and 8.28% lower seed oil content compared to WT”
Claim · model-written summary
Seed oil fatty acid composition was significantly altered in transgenic Jatropha lines with rJcSPL9 or JcmiR156a
- Material
- rJcSPL9 and JcmiR156a transgenice6
- Outcome
- seed oil fatty acid compositione6
- Direction
- significantly alterede6
JatrophaOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Jatropha curcas is a promising feedstock for biodiesel and bio-jet fuels production”
e6“Additionally, seed oil fatty acid composition was significantly altered in both rJcSPL9 and JcmiR156a transgenic Jatropha and Arabidopsis, as well as in Arabidopsis spl9 mutants”
Claim · model-written summary
Seed oil fatty acid composition was significantly altered in Arabidopsis spl9 mutants
- Taxon
- Arabidopsis
Arabidopsise6 - Material
- spl9 mutantse6
- Outcome
- seed oil fatty acid compositione6
- Direction
- significantly alterede6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“Additionally, seed oil fatty acid composition was significantly altered in both rJcSPL9 and JcmiR156a transgenic Jatropha and Arabidopsis, as well as in Arabidopsis spl9 mutants”
Claim · model-written summary
Oil biosynthesis genes JcWRI1, JcDGAT1 and JcDGAT2 were upregulated in rJcSPL9 transgenic seeds
- Taxon
- Jatropha curcasJatropha curcase0
- Material
- rJcSPL9 transgenice7
- Organ / stage
- seedse7
- Gene
- JcWRI1, JcDGAT1, JcDGAT2e7
- Outcome
- oil biosynthesis genese7
- Direction
- upregulatede7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Jatropha curcas is a promising feedstock for biodiesel and bio-jet fuels production”
e7“The key oil biosynthesis genes, including JcWRI1, JcDGAT1, JcDGAT2, and JcOLEOSIN, were upregulated in rJcSPL9 transgenic seeds but downregulated in JcmiR156a transformants”
Claim · model-written summary
Oil biosynthesis gene JcOLEOSIN was upregulated in rJcSPL9 transgenic seeds
- Taxon
- Jatropha curcasJatropha curcase0
- Material
- rJcSPL9 transgenice7
- Organ / stage
- seedse7
- Gene
- JcOLEOSINe7
- Outcome
- oil biosynthesis genese7
- Direction
- upregulatede7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Jatropha curcas is a promising feedstock for biodiesel and bio-jet fuels production”
e7“The key oil biosynthesis genes, including JcWRI1, JcDGAT1, JcDGAT2, and JcOLEOSIN, were upregulated in rJcSPL9 transgenic seeds but downregulated in JcmiR156a transformants”
Claim · model-written summary
Oil biosynthesis genes JcWRI1, JcDGAT1 and JcDGAT2 were downregulated in JcmiR156a transformants
- Taxon
- Jatropha curcasJatropha curcase0
- Material
- JcmiR156a transformantse7
- Gene
- JcWRI1, JcDGAT1, JcDGAT2e7
- Outcome
- oil biosynthesis genese7
- Direction
- downregulatede7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Jatropha curcas is a promising feedstock for biodiesel and bio-jet fuels production”
e7“The key oil biosynthesis genes, including JcWRI1, JcDGAT1, JcDGAT2, and JcOLEOSIN, were upregulated in rJcSPL9 transgenic seeds but downregulated in JcmiR156a transformants”
Claim · model-written summary
Oil biosynthesis gene JcOLEOSIN was downregulated in JcmiR156a transformants
- Taxon
- Jatropha curcasJatropha curcase0
- Material
- JcmiR156a transformantse7
- Gene
- JcOLEOSINe7
- Outcome
- oil biosynthesis genese7
- Direction
- downregulatede7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Jatropha curcas is a promising feedstock for biodiesel and bio-jet fuels production”
e7“The key oil biosynthesis genes, including JcWRI1, JcDGAT1, JcDGAT2, and JcOLEOSIN, were upregulated in rJcSPL9 transgenic seeds but downregulated in JcmiR156a transformants”
Claim · model-written summary
The miR156/SPL9 module regulates lipid accumulation and fatty acid biosynthesis in Jatropha seeds
- Taxon
- Jatropha curcasJatropha curcase0
- Gene
- miR156/SPL9 modulee7
- Outcome
- lipid accumulation and fatty acid biosynthesis in seedse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Jatropha curcas is a promising feedstock for biodiesel and bio-jet fuels production”
e7“This study provides the first evidence that the miR156/SPL9 module regulates lipid accumulation and fatty acid biosynthesis in seeds”
Claim · model-written summary
SPL9 is a promising target for enhancing oil yield and quality in Jatropha and other oilseed crops
- Outcome
- enhancing oil yield and qualitye7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“These results highlight SPL9 as a promising target for enhancing oil yield and quality in Jatropha and other oilseed crops”