Abstract
Background/Objectives: Soil salinization and alkalization critically limit global agricultural production. This study aimed to investigate the differential response mechanisms of rapeseed (Brassica napus L.) varieties to saline and alkaline stresses at the seedling stage. Methods: Seedlings of a salt-tolerant variety, Huayouza 62 (H62), and a non-salt-tolerant variety, Xiangyou 15 (X15), were exposed to saline (NaCl:Na2SO4 = 1:1) and alkaline (Na2CO3:NaHCO3 = 1:1) stresses. An integrated analysis combining physiology, biochemistry, transcriptomics, and metabolomics was conducted to systematically elucidate their differential stress responses. Results: (1) H62 maintained favorable photosynthetic and carbon–nitrogen homeostasis. Notably, under saline and alkaline stresses, the activity of glutamate dehydrogenase (GDH) in H62 showed a significant increasing trend, whereas it was inhibited in X15. (2) Alkaline stress triggered more differential genes than saline stress, with H62 exhibiting broader transcriptional up-regulation in carbon–nitrogen metabolism. (3) Metabolomic profiling showed that H62 accumulated more beneficial metabolites than X15 under both stresses, such as phenolic acids, amino acids, and their derivatives. (4) In multi-omics analysis, key genes in starch–sucrose and amino acid metabolism in H62 were up-regulated to accumulate osmolytes, enabling an efficient defense network. However, X15’s responses were disordered. Conclusions: H62 leverages robust transcriptional reprogramming to coordinate carbon–nitrogen metabolism, constituting a multidimensional defense network. This study provides potential physiological indicators, candidate genes, and metabolite markers associated with short-term saline–alkaline stress responses, laying a foundation for further exploration of stress response mechanisms.
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Claims 8
Claim · model-written summary
H62 maintained favorable photosynthetic and carbon–nitrogen homeostasis.
- Taxon
- rapeseed (Brassica napus L.)
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napust0 - Material
- H62e0
- Outcome
- photosynthetic and carbon–nitrogen homeostasise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
e0“H62 maintained favorable photosynthetic and carbon–nitrogen homeostasis.”
t0“rapeseed (Brassica napus L.)”
Claim · model-written summary
Saline and alkaline stresses significantly increased GDH activity in H62.
- Taxon
- rapeseed (Brassica napus L.)Brassica napust0
- Material
- H62e0
- Treatment
- saline and alkaline stressese0
- Gene
- glutamate dehydrogenase (GDH)e0
- Outcome
- activity of glutamate dehydrogenase (GDH)e0
- Direction
- increasinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“under saline and alkaline stresses, the activity of glutamate dehydrogenase (GDH) in H62 showed a significant increasing trend”
t0“rapeseed (Brassica napus L.)”
Claim · model-written summary
Saline and alkaline stresses inhibited GDH activity in X15.
- Taxon
- rapeseed (Brassica napus L.)Brassica napust0
- Material
- X15e0
- Treatment
- saline and alkaline stressese1
- Outcome
- glutamate dehydrogenase (GDH)e1
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“whereas it was inhibited in X15”
t0“rapeseed (Brassica napus L.)”
e1“under saline and alkaline stresses, the activity of glutamate dehydrogenase (GDH) in H62 showed a significant increasing trend”
Claim · model-written summary
Alkaline stress triggered more differential genes than saline stress.
- Taxon
- rapeseed (Brassica napus L.)Brassica napust0
- Treatment
- Alkaline stresse0
- Outcome
- differential genese0
- Direction
- moree0
- Comparator
- saline stresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Alkaline stress triggered more differential genes than saline stress”
t0“rapeseed (Brassica napus L.)”
Claim · model-written summary
H62 showed broader transcriptional up-regulation of carbon–nitrogen metabolism.
- Taxon
- rapeseed (Brassica napus L.)Brassica napust0
- Material
- H62e0
- Outcome
- transcriptional up-regulation in carbon–nitrogen metabolisme0
- Direction
- up-regulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“H62 exhibiting broader transcriptional up-regulation in carbon–nitrogen metabolism”
t0“rapeseed (Brassica napus L.)”
Claim · model-written summary
H62 accumulated more phenolic acids, amino acids, and derivatives than X15 under both stresses.
- Taxon
- rapeseed (Brassica napus L.)Brassica napust0
- Material
- H62e0
- Treatment
- both stressese0
- Outcome
- phenolic acids, amino acids, and their derivativese0
- Direction
- moree0
- Comparator
- X15e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“H62 accumulated more beneficial metabolites than X15 under both stresses, such as phenolic acids, amino acids, and their derivatives.”
t0“rapeseed (Brassica napus L.)”
Claim · model-written summary
H62 up-regulated starch–sucrose and amino acid metabolism genes to accumulate osmolytes.
- Taxon
- rapeseed (Brassica napus L.)Brassica napust0
- Material
- H62e0
- Gene
- key genes in starch–sucrose and amino acid metabolisme0
- Outcome
- accumulate osmolytese0
- Direction
- up-regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“key genes in starch–sucrose and amino acid metabolism in H62 were up-regulated to accumulate osmolytes”
t0“rapeseed (Brassica napus L.)”
Claim · model-written summary
X15 stress responses were disordered.
- Taxon
- rapeseed (Brassica napus L.)Brassica napust0
- Material
- X15e0
- Outcome
- responses were disorderede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“However, X15’s responses were disordered.”
t0“rapeseed (Brassica napus L.)”
The same questions in other papers
salinity · molecular: metabolites · Brassica napus (oilseed rape)Positive and negative results
2 papers in the same direction as this paper · 14 in the opposite direction
- no effect
- negative
- negative
13 papers more in this group
See all 17 papers →alkalinity · molecular: metabolites · Brassica napus (oilseed rape)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.