Paper · 2026 · Journal of Sustainable Agriculture and Environment

Toward Sustainable Fertilization: Biochar and Plant Growth Promoting Bacteria (PGPB) Influence on Sweet Pepper ( Capsicuum annuum L.) Development and Production Under Reduced Nitrogen Input

Angela Libutti; Annalisa d'Amelio; Muhammad Zubair Akram; et al.

7
claims
13
verbatim quotes
0
citations
3
research areas
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Open access · cc-by

Abstract

ABSTRACT Soil amendment with biochar and PGPB represents a promising strategy to reduce dependence on chemical fertilizers. This study aimed to evaluate the potential of biochar and PGPB application, under a reduced nitrogen fertilization, to sustain sweet pepper growth and production, soil chemical and microbiological fertility. Plants were grown in pots under greenhouse on soil un‐amended or amended with 2% biochar (B0 and B2), un‐inoculated or inoculated with PGPB (P0 and P+) and un‐fertilized or fertilized with 50 (half dose) or 100 (full dose) kg N ha −1 (N0, N50 and N100). Plant above‐ and below‐ground biomass development, soil chemical and microbiological properties, and fruit quanti‐qualitative traits were assessed during and/or at the end of two periods: from transplanting (T0) to flowering initiation (T1) and from flowering initiation to harvest (T2). Under B2‐P+ treatment, N50 fertilized plants exhibited growth similar to N100, with 50% higher height and more than doubled leaf number compared with N0. At T2, the Absolute Growth Rate of plant height increased by 12% under B2 compared with B0, by 45% under P+ compared with T1, while the Absolute Growth Rate of leaf number was 60% higher under B2 compared with T1. Biochar and PGPB resulted in higher N tissue concentrations (+66 and +34% in stem; +16 and +11% in leaves, respectively), root length (+27%) and biomass (+170%), respectively. At soil level, B2 increased carbon and nitrogen contents by 94% and 25% compared with B0; P+ resulted in 12% higher carbon content, compared with P‐; half N fertilization favoured microbial growth. N50 fertilized plants showed fruit yield, weight, nitrogen and carbon contents comparable to N100. Biochar and PGPB application with half N dose maintained pepper growth and yield at levels comparable to full N fertilization, while improving soil fertility and exerting a modulatory effect on specific soil bacterial families.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 7

Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Half-dose N (N50) plants showed fruit yield, weight, nitrogen and carbon contents comparable to full-dose N100.

Taxon
Sweet PepperCapsicum annuumt0
Treatment
N50e0
Outcome
fruit yield, weight, nitrogen and carbon contentse0
Direction
comparablee0
Comparator
N100e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsicum annuum
Capsicum annuum · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“N50 fertilized plants showed fruit yield, weight, nitrogen and carbon contents comparable to N100”

✓ located verbatim in the source · abstract, characters 1616–1713 · show in text ↑
t0

“Sweet Pepper”

✓ located verbatim in the source · title, characters 98–110 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Soil amended with 2% biochar (B2) had 94% higher carbon content than unamended soil (B0).

Taxon
Sweet PepperCapsicum annuumt0
Treatment
B2e0
Outcome
carbone0
Direction
increasede0
Value
94%e0
Comparator
B0e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsicum annuum
Capsicum annuum · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“B2 increased carbon and nitrogen contents by 94% and 25% compared with B0”

✓ located verbatim in the source · abstract, characters 1433–1506 · show in text ↑
t0

“Sweet Pepper”

✓ located verbatim in the source · title, characters 98–110 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Soil amended with 2% biochar (B2) had 25% higher nitrogen content than unamended soil (B0).

Taxon
Sweet PepperCapsicum annuumt0
Treatment
B2e0
Outcome
nitrogen contentse0
Direction
increasede0
Value
25%e0
Comparator
B0e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsicum annuum
Capsicum annuum · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“B2 increased carbon and nitrogen contents by 94% and 25% compared with B0”

✓ located verbatim in the source · abstract, characters 1433–1506 · show in text ↑
t0

“Sweet Pepper”

✓ located verbatim in the source · title, characters 98–110 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Biochar and PGPB led to higher N tissue concentrations (+66 and +34% in stem).

Taxon
Sweet PepperCapsicum annuumt0
Treatment
Biochar and PGPBe0
Outcome
N tissue concentrationse0
Direction
highere0
Value
+66 and +34% in steme0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsicum annuum
Capsicum annuum · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Biochar and PGPB resulted in higher N tissue concentrations (+66 and +34% in stem; +16 and +11% in leaves, respectively), root length (+27%) and biomass (+170%), respectively”

✓ located verbatim in the source · abstract, characters 1242–1416 · show in text ↑
t0

“Sweet Pepper”

✓ located verbatim in the source · title, characters 98–110 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Biochar and PGPB led to greater root length (+27%).

Taxon
Sweet PepperCapsicum annuumt0
Treatment
Biochar and PGPBe0
Outcome
root lengthe0
Direction
highere0
Value
+27%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsicum annuum
Capsicum annuum · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Biochar and PGPB resulted in higher N tissue concentrations (+66 and +34% in stem; +16 and +11% in leaves, respectively), root length (+27%) and biomass (+170%), respectively”

✓ located verbatim in the source · abstract, characters 1242–1416 · show in text ↑
t0

“Sweet Pepper”

✓ located verbatim in the source · title, characters 98–110 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Biochar and PGPB led to greater biomass (+170%).

Taxon
Sweet PepperCapsicum annuumt0
Treatment
Biochar and PGPBe0
Outcome
biomasse0
Direction
highere0
Value
+170%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsicum annuum
Capsicum annuum · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Biochar and PGPB resulted in higher N tissue concentrations (+66 and +34% in stem; +16 and +11% in leaves, respectively), root length (+27%) and biomass (+170%), respectively”

✓ located verbatim in the source · abstract, characters 1242–1416 · show in text ↑
t0

“Sweet Pepper”

✓ located verbatim in the source · title, characters 98–110 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Biochar and PGPB with half N dose maintained pepper growth and yield at levels comparable to full N fertilization.

Taxon
peppere0
Treatment
Biochar and PGPB application with half N dosee0
Interactor
PGPBe0symbiont
Outcome
pepper growth and yielde0
Direction
comparablee0
Comparator
full N fertilizatione0

Verbatim

e0

“Biochar and PGPB application with half N dose maintained pepper growth and yield at levels comparable to full N fertilization”

✓ located verbatim in the source · abstract, characters 1715–1840 · show in text ↑

biochar · growth · Capsicum annuum (sweet pepper)Positive and negative results

10 papers: 7 positive, 3 negative or no effect

6 papers in the same direction as this paper · 3 in the opposite direction

  1. no effect
  2. no effect
  3. negative

6 papers more in this group

See all 10 papers →

inoculation · growth · Capsicum annuum (sweet pepper)Positive and negative results

52 papers: 48 positive, 4 negative or no effect

47 papers in the same direction as this paper · 4 in the opposite direction

  1. no effect
  2. negative
  3. no effect

48 papers more in this group

See all 52 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.