Abstract
Salt stress severely impairs crop productivity worldwide. MicroRNAs (miRNAs) are a class of endogenous small noncoding RNAs, which played the crucial role in regulating plant growth, development as well as stress responses at the posttranscriptional level. However, the significance of miRNA on salt response in wheat is not well understood at present. In this study, we identified a salt-responsive miRNA from wild emmer wheat, miR9772, which appears to be specific to Triticum species. Under salt stress, the expression of miR9772 was significantly induced and upregulated. Functional analyses revealed that overexpression of miR9772 increased salt sensitivity in wheat, whereas silencing of miR9772 using Short Tandem Target Mimic (STTM) technology markedly enhanced salt tolerance, demonstrated its crucial role in regulating wheat's salt response. Furthermore, we revealed that miR9772 could target on CYP76C4 to decline its expression abundance to affect wheat's salt resistance. Additionally, agronomic and yield-related traits of transgenic wheat lines based on field experiments showed that miR9772-silenced lines exhibited larger grain size and higher grain yield per plant, indicating that miR9772 simultaneously regulated the salt tolerance and grain development. Collectively, this study provided a new target for improving wheat salt tolerance without yield penalty through genome editing breeding.
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Claims 8
Claim · model-written summary
miR9772 was identified as salt responsive and apparently Triticum specific.
- Taxon
- wild emmer wheat
Triticum dicoccoidese0 - Gene
- miR9772e0
- Outcome
- salt-responsive miRNAe0
- Qualifier
- appears to be specific to Triticum speciese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified a salt-responsive miRNA from wild emmer wheat, miR9772, which appears to be specific to Triticum species.”
Claim · model-written summary
Salt stress significantly induced miR9772 expression.
TriticumNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under salt stress, the expression of miR9772 was significantly induced and upregulated.”
t0“Triticum”
Claim · model-written summary
miR9772 overexpression increased wheat salt sensitivity.
- Taxon
- wheat
Triticum aestivume0 - Treatment
- overexpression of miR9772e0
- Gene
- miR9772e0
- Outcome
- salt sensitivitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of miR9772 increased salt sensitivity in wheat”
Claim · model-written summary
miR9772 silencing enhanced wheat salt tolerance.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- silencing of miR9772e0
- Gene
- miR9772e0
- Outcome
- salt tolerancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of miR9772 using Short Tandem Target Mimic (STTM) technology markedly enhanced salt tolerance”
t0“wheat”
Claim · model-written summary
miR9772 targeted CYP76C4 and decreased its expression.
- Taxon
- wheatTriticum aestivume0
- Gene
- CYP76C4e0
- Outcome
- expression abundancee0
- Qualifier
- miR9772 could target on CYP76C4e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“miR9772 could target on CYP76C4 to decline its expression abundance to affect wheat's salt resistance.”
Claim · model-written summary
miR9772-silenced lines had larger grain size.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- miR9772-silenced linese0
- Outcome
- grain sizee0
- Direction
- largere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“miR9772-silenced lines exhibited larger grain size and higher grain yield per plant”
t0“wheat”
Claim · model-written summary
miR9772-silenced lines had higher grain yield per plant.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- miR9772-silenced linese0
- Outcome
- grain yield per plante0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“miR9772-silenced lines exhibited larger grain size and higher grain yield per plant”
t0“wheat”
Claim · model-written summary
The authors propose miR9772 as an editing target for salt tolerance without yield penalty.
- Taxon
- wheatTriticum aestivume0
- Outcome
- improving wheat salt tolerance without yield penalty through genome editing breedinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a new target for improving wheat salt tolerance without yield penalty through genome editing breeding”
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See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.