Abstract
Abstract Reynoutria japonica , known for its exceptional growth capacity and tolerance to multiple abiotic stresses, represents an ideal model for studying the trade-off between plant growth and stress adaptation. However, the molecular mechanisms underlying the balance between plant growth and stress resistance in R. japonica remain unclear. Increasing evidence indicates that WRKY proteins play a direct role in regulating the equilibrium between plant growth and resistance. In this study, we identified and conducted bioinformatics analysis about WRKY gene family from two haplotype chromosomes (HapA 89, HapE 82) in R. japonica . These 171 RjWRKY members distributed in three groups: group I (34), group II (115), and group III (22). RjWRKY members within the same group or subgroup typically exhibited similar conserved motifs and gene structures. Promoter sequences in the WRKY gene family contained a series of cis -acting elements related to plant growth and development, stress response, and hormone response. Based on transcriptome data, we screened 12 candidate RjWRKY genes that are associated with stem elongation and are simultaneously responsive to methyl jasmonate (MeJA, a stress-response ‘messenger’) and ultraviolet (UV) radiation. Further, we confirmed the relative expression levels of 12 RjWRKY genes during different stem-growth stages as well as under auxin (IAA) or auxin antagonist (PEO-IAA), salt, cadmium (Cd) and manganese(Mn) treatments by qRT-PCR. The 12 RjWRKY genes, responsive to both auxin signals and abiotic stress, may thus represent key regulators mediating the trade-off between plant growth and stress in this invasive plant species. In addition, overexpression of HuZ00251595.1 (a homology of AtWRKY11, designated as RjWRKY11) in A. thaliana significantly enhanced resistance to Cd. These results establish a solid foundation for further exploration into the functions of the RjWRKYs in the balance of growth and adaptation to various abiotic stresses in R. japonica .
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Claims 12
Claim · model-written summary
171 RjWRKY members were identified from the two haplotype chromosomes of R. japonica.
- Taxon
- Reynoutria japonica
Reynoutria japonicat0 - Gene
- RjWRKYe0
- Outcome
- RjWRKY memberse0
- Value
- 171e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Reynoutria japonica”
e0“These 171 RjWRKY members distributed in three groups”
Claim · model-written summary
RjWRKY group I contains 34 members.
- Taxon
- Reynoutria japonicaReynoutria japonicat0
- Outcome
- group Ie0
- Value
- 34e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Reynoutria japonica”
e0“group I (34)”
Claim · model-written summary
RjWRKY group II contains 115 members.
- Taxon
- Reynoutria japonicaReynoutria japonicat0
- Outcome
- group IIe0
- Value
- 115e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Reynoutria japonica”
e0“group II (115)”
Claim · model-written summary
RjWRKY group III contains 22 members.
- Taxon
- Reynoutria japonicaReynoutria japonicat0
- Outcome
- group IIIe0
- Value
- 22e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Reynoutria japonica”
e0“group III (22)”
Claim · model-written summary
RjWRKY members of the same group or subgroup typically have similar conserved motifs and gene structures.
- Taxon
- Reynoutria japonicaReynoutria japonicat0
- Gene
- RjWRKYe0
- Outcome
- conserved motifs and gene structurese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Reynoutria japonica”
e0“RjWRKY members within the same group or subgroup typically exhibited similar conserved motifs and gene structures”
Claim · model-written summary
Promoters of the WRKY gene family contain cis-acting elements related to growth and development, stress response and hormone response.
- Taxon
- Reynoutria japonicaReynoutria japonicat0
- Gene
- WRKY gene familye0
- Outcome
- cis -acting elementse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Reynoutria japonica”
e0“Promoter sequences in the WRKY gene family contained a series of cis -acting elements related to plant growth and development, stress response, and hormone response”
Claim · model-written summary
Transcriptome data identified 12 candidate RjWRKY genes associated with stem elongation.
- Taxon
- Reynoutria japonicaReynoutria japonicat0
- Gene
- RjWRKY genese0
- Outcome
- associated with stem elongatione0
- Value
- 12e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Reynoutria japonica”
e0“we screened 12 candidate RjWRKY genes that are associated with stem elongation”
Claim · model-written summary
The candidate RjWRKY genes are responsive to methyl jasmonate.
- Taxon
- Reynoutria japonicaReynoutria japonicat0
- Treatment
- methyl jasmonatee0
- Outcome
- simultaneously responsivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Reynoutria japonica”
e0“are simultaneously responsive to methyl jasmonate (MeJA, a stress-response ‘messenger’) and ultraviolet (UV) radiation”
Claim · model-written summary
The candidate RjWRKY genes are responsive to ultraviolet radiation.
- Taxon
- Reynoutria japonicaReynoutria japonicat0
- Treatment
- ultraviolet (UV) radiatione0
- Outcome
- simultaneously responsivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Reynoutria japonica”
e0“are simultaneously responsive to methyl jasmonate (MeJA, a stress-response ‘messenger’) and ultraviolet (UV) radiation”
Claim · model-written summary
The 12 RjWRKY genes respond to both auxin signals and abiotic stress.
- Taxon
- Reynoutria japonicaReynoutria japonicat0
- Gene
- RjWRKY genese0
- Outcome
- responsive to both auxin signals and abiotic stresse0
- Value
- 12e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Reynoutria japonica”
e0“The 12 RjWRKY genes, responsive to both auxin signals and abiotic stress”
Claim · model-written summary
The authors suggest the 12 RjWRKY genes may be key regulators mediating the trade-off between plant growth and stress.
- Taxon
- Reynoutria japonicaReynoutria japonicat0
- Gene
- RjWRKY genese0
- Outcome
- trade-off between plant growth and stresse1
- Qualifier
- key regulatorse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Reynoutria japonica”
e0“The 12 RjWRKY genes”
e1“may thus represent key regulators mediating the trade-off between plant growth and stress”
Claim · model-written summary
Overexpression of HuZ00251595.1 (RjWRKY11) in A. thaliana significantly enhanced resistance to Cd.
- Taxon
- A. thalianae0
- Treatment
- overexpression of HuZ00251595.1e0
- Gene
- HuZ00251595.1e0
- Outcome
- resistance to Cde0
- Direction
- enhancede0
Not reportedComparator
Verbatim
e0“overexpression of HuZ00251595.1 (a homology of AtWRKY11, designated as RjWRKY11) in A. thaliana significantly enhanced resistance to Cd”