Abstract
The Dof transcription factor family plays crucial roles in plant growth and stress responses. In this study, we identified 24 Dof genes (CcDof1–CcDof24) from the genome of Citrus clementina (Hort. ex Tan.). Phylogenetic analysis classified these proteins into six distinct clades, revealing evolutionary conservation with Dof members from Arabidopsis and tomato. Analysis of gene structure and conserved motifs showed that most CcDof genes are intronless or contain only a few introns, and their motif compositions are largely consistent with their phylogenetic relationships. Promoter analysis revealed a variety of cis-regulatory elements associated with light responsiveness, hormone signaling, and abiotic/biotic stress responses. Expression profiling demonstrated that CcDof genes exhibit tissue-specific expression patterns and are differentially regulated by various phytohormones (including ABA, SA, GA, and MeJA), low temperature stress, and infection by Phytophthora parasitica. Notably, transient overexpression of CcDof4 and CcDof6 in citrus leaves significantly enhanced resistance to P. parasitica, accompanied by upregulation of SA pathway markers NPR1 and PR1. Our findings provide a systematic characterization of the CcDof family and highlight the important roles of CcDof4 and CcDof6 in mediating citrus disease resistance, likely through modulation of the SA signaling pathway.
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Claims 13
Claim · model-written summary
Twenty-four Dof genes (CcDof1-CcDof24) were identified from the Citrus clementina genome.
- Taxon
- Citrus clementina
Citrus ×clementinae0 - Gene
- CcDof1–CcDof24e0
- Outcome
- 24 Dof genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified 24 Dof genes (CcDof1–CcDof24) from the genome of Citrus clementina (Hort. ex Tan.)”
Claim · model-written summary
Phylogenetic analysis classified the Citrus clementina Dof proteins into six distinct clades.
- Taxon
- Citrus clementinaCitrus ×clementinat0
- Outcome
- six distinct cladese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phylogenetic analysis classified these proteins into six distinct clades”
t0“Citrus clementina”
Claim · model-written summary
The Citrus clementina Dof proteins showed evolutionary conservation with Dof members from Arabidopsis and tomato.
- Taxon
- Citrus clementinaCitrus ×clementinat0
- Outcome
- evolutionary conservatione0
- Comparator
- Arabidopsis and tomatoe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“revealing evolutionary conservation with Dof members from Arabidopsis and tomato”
t0“Citrus clementina”
Claim · model-written summary
Most CcDof genes are intronless or contain only a few introns.
- Taxon
- Citrus clementinaCitrus ×clementinat0
- Gene
- CcDof genese0
- Outcome
- intronless or contain only a few intronse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“most CcDof genes are intronless or contain only a few introns”
t0“Citrus clementina”
Claim · model-written summary
Promoter analysis revealed cis-regulatory elements associated with light responsiveness, hormone signaling and abiotic/biotic stress responses.
- Taxon
- Citrus clementinaCitrus ×clementinat0
- Outcome
- cis-regulatory elementse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Promoter analysis revealed a variety of cis-regulatory elements associated with light responsiveness, hormone signaling, and abiotic/biotic stress responses”
t0“Citrus clementina”
Claim · model-written summary
CcDof genes exhibit tissue-specific expression patterns.
- Taxon
- Citrus clementinaCitrus ×clementinat0
- Gene
- CcDof genese0
- Outcome
- tissue-specific expression patternse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CcDof genes exhibit tissue-specific expression patterns”
t0“Citrus clementina”
Claim · model-written summary
CcDof genes are differentially regulated by various phytohormones (ABA, SA, GA, MeJA).
- Taxon
- Citrus clementinaCitrus ×clementinat0
- Treatment
- various phytohormones (including ABA, SA, GA, and MeJA)e0
- Outcome
- differentially regulatede0
- Direction
- differentially regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“differentially regulated by various phytohormones (including ABA, SA, GA, and MeJA), low temperature stress, and infection by Phytophthora parasitica”
t0“Citrus clementina”
Claim · model-written summary
CcDof genes are differentially regulated by low temperature stress.
- Taxon
- Citrus clementinaCitrus ×clementinat0
- Treatment
- low temperature stresse0
- Outcome
- differentially regulatede0
- Direction
- differentially regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“differentially regulated by various phytohormones (including ABA, SA, GA, and MeJA), low temperature stress, and infection by Phytophthora parasitica”
t0“Citrus clementina”
Claim · model-written summary
CcDof genes are differentially regulated by infection with Phytophthora parasitica.
- Taxon
- Citrus clementinaCitrus ×clementinat0
- Treatment
- infection by Phytophthora parasiticae0
- Interactor
- Phytophthora parasitica
Phytophthora nicotianaee0pathogen - Outcome
- differentially regulatede0
- Direction
- differentially regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“differentially regulated by various phytohormones (including ABA, SA, GA, and MeJA), low temperature stress, and infection by Phytophthora parasitica”
t0“Citrus clementina”
Claim · model-written summary
Transient overexpression of CcDof4 in citrus leaves significantly enhanced resistance to P. parasitica.
- Organ / stage
- leavese0
- Treatment
- transient overexpressione0
- Interactor
- P. parasiticaPhytophthora nicotianaee0pathogen
- Gene
- CcDof4e0
- Outcome
- resistance to P. parasiticae0
- Direction
- enhancede0
CitrusNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transient overexpression of CcDof4 and CcDof6 in citrus leaves significantly enhanced resistance to P. parasitica”
Claim · model-written summary
Transient overexpression of CcDof6 in citrus leaves significantly enhanced resistance to P. parasitica.
- Organ / stage
- leavese0
- Treatment
- transient overexpressione0
- Interactor
- P. parasiticaPhytophthora nicotianaee0pathogen
- Gene
- CcDof6e0
- Outcome
- resistance to P. parasiticae0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transient overexpression of CcDof4 and CcDof6 in citrus leaves significantly enhanced resistance to P. parasitica”
Claim · model-written summary
Overexpression of CcDof4 and CcDof6 in citrus leaves was accompanied by upregulation of the SA pathway markers NPR1 and PR1.
- Organ / stage
- leavese0
- Treatment
- transient overexpression of CcDof4 and CcDof6e0
- Outcome
- SA pathway markers NPR1 and PR1e0
- Direction
- upregulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transient overexpression of CcDof4 and CcDof6 in citrus leaves significantly enhanced resistance to P. parasitica, accompanied by upregulation of SA pathway markers NPR1 and PR1”
Claim · model-written summary
The authors interpret CcDof4 and CcDof6 as mediating citrus disease resistance, likely through modulation of the SA signaling pathway.
- Gene
- CcDof4 and CcDof6e0
- Outcome
- citrus disease resistancee0
- Qualifier
- modulation of the SA signaling pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“highlight the important roles of CcDof4 and CcDof6 in mediating citrus disease resistance, likely through modulation of the SA signaling pathway”