Abstract
Biotic and abiotic stresses alter the physiology of perennial plants, with consequences for fungal endophytes and disease expression. In grapevine, drought inhibits esca disease expression, but the underlying molecular interactions between the plant and fungi are unknown. We combined wood metatranscriptomics, metabolomics, and metabarcoding to investigate these interactions in 30-year-old grapevines and eight wood-pathogenic fungi under conditions of drought or esca leaf symptom expression. Both esca and drought decreased grapevine transpiration, but with different underlying mechanisms that induced specific transcriptomic and metabolic signatures. Similar pathways were also activated, including the phenylpropanoid and stilbenoid synthesis pathways. These stress responses could potentially confer cross-tolerance and elicit different fungal molecular responses. Across all fungi, the total level of putative virulence factors increased significantly under both stresses. Under drought, only the relative abundance of Phaeomoniella chlamydospora and gene expression involved in anti-oxidative mechanisms, growth, and reproduction increased. Under esca expression conditions, only the relative abundance of Fomitiporia mediterranea and gene expression involved in wood degradation, competition, detoxification, and growth increased. Under drought, induced grapevine defenses and reduced transpiration, together with the low abundance and putatively weak virulence of F. mediterranea may account for the inhibition of esca leaf symptom.
Abstract from the paper’s OpenAlex record; open copy licensed other-oa. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 10
Claim · model-written summary
Esca reduced grapevine transpiration.
VitisOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both esca and drought decreased grapevine transpiration”
Claim · model-written summary
Drought reduced grapevine transpiration.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both esca and drought decreased grapevine transpiration”
Claim · model-written summary
Esca and drought produced distinct transcriptomic and metabolic signatures through different underlying mechanisms.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but with different underlying mechanisms that induced specific transcriptomic and metabolic signatures”
e1“Both esca and drought decreased grapevine transpiration”
Claim · model-written summary
Both stresses activated phenylpropanoid and stilbenoid synthesis pathways.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Similar pathways were also activated, including the phenylpropanoid and stilbenoid synthesis pathways”
e1“Both esca and drought decreased grapevine transpiration”
Claim · model-written summary
Across fungi, putative virulence factors increased significantly under esca and drought.
- Treatment
- both stressese0
- Interactor
- all fungie0pathogen
- Outcome
- total level of putative virulence factorse0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Across all fungi, the total level of putative virulence factors increased significantly under both stresses”
e1“Both esca and drought decreased grapevine transpiration”
Claim · model-written summary
Drought increased relative abundance of Phaeomoniella chlamydospora.
- Treatment
- droughte0
- Interactor
- Phaeomoniella chlamydosporaPhaeomoniella chlamydosporae0pathogen
- Outcome
- relative abundance of Phaeomoniella chlamydosporae0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under drought, only the relative abundance of Phaeomoniella chlamydospora and gene expression involved in anti-oxidative mechanisms, growth, and reproduction increased”
e1“Both esca and drought decreased grapevine transpiration”
Claim · model-written summary
Under drought, fungal expression for antioxidative mechanisms, growth, and reproduction increased.
- Treatment
- droughte0
- Interactor
- Phaeomoniella chlamydosporaPhaeomoniella chlamydosporae0pathogen
- Outcome
- gene expression involved in anti-oxidative mechanisms, growth, and reproductione0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under drought, only the relative abundance of Phaeomoniella chlamydospora and gene expression involved in anti-oxidative mechanisms, growth, and reproduction increased”
e1“Both esca and drought decreased grapevine transpiration”
Claim · model-written summary
Esca expression increased relative abundance of Fomitiporia mediterranea.
- Treatment
- esca expression conditionse0
- Interactor
- Fomitiporia mediterranea
Fomitiporia mediterraneae0pathogen - Outcome
- relative abundance of Fomitiporia mediterraneae0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under esca expression conditions, only the relative abundance of Fomitiporia mediterranea and gene expression involved in wood degradation, competition, detoxification, and growth increased”
e1“Both esca and drought decreased grapevine transpiration”
Claim · model-written summary
Under esca expression, fungal gene expression for wood degradation, competition, detoxification, and growth increased.
- Treatment
- esca expression conditionse0
- Interactor
- Fomitiporia mediterraneaFomitiporia mediterraneae0pathogen
- Outcome
- gene expression involved in wood degradation, competition, detoxification, and growthe0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under esca expression conditions, only the relative abundance of Fomitiporia mediterranea and gene expression involved in wood degradation, competition, detoxification, and growth increased”
e1“Both esca and drought decreased grapevine transpiration”
Claim · model-written summary
Induced grapevine defenses, lower transpiration, and low F. mediterranea abundance and virulence may explain drought inhibition of esca leaf symptoms.
- Treatment
- droughte0
- Interactor
- F. mediterraneaFomitiporia mediterraneae0pathogen
- Outcome
- inhibition of esca leaf symptome0
- Qualifier
- induced grapevine defenses and reduced transpiratione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under drought, induced grapevine defenses and reduced transpiration, together with the low abundance and putatively weak virulence of F. mediterranea may account for the inhibition of esca leaf symptom”
The same questions in other papers
drought · physiology: gas exchange · VitisPositive and negative results
14 papers in the same direction as this paper · 3 in the opposite direction
- positive
- positive
- positive
14 papers more in this group
See all 18 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.