Abstract
Soil salinization is one of the major abiotic stresses that influences agricultural production and the environment. Auxin response factors (ARFs) are key components of the auxin signal transduction pathway, while their role in wheat salt stress responses remains unclear. In this study, we identified TaARF18 as a negative regulator of salt tolerance in wheat. The coding sequences of TaARF18-A, TaARF18-B, and TaARF18-D were 2106, 2088, and 2088 bp, respectively. TaARF18 is a hydrophilic protein featuring typical Auxin-resp and B3 DNA-binding domains and exhibits relatively high evolutionary conservation among Poaceae species. The expression of TaARF18 was upregulated under salt stress. TaARF18 predominantly accumulated in the nucleus. Silencing of TaARF18 via the BSMV-VIGS approach enhanced salt tolerance in wheat seedlings. In addition, haplotype analysis based on resequencing data from 355 wheat accessions identified 25, 31, and 16 haplotypes for TaARF18-A, TaARF18-B, and TaARF18-D, respectively. Fourteen wheat accessions carrying different haplotypes were evaluated under salt stress, and HapIII of TaARF18-A exhibited the highest level of salt tolerance, which can act as a strong selection locus in global wheat breeding. Our findings provide insight into the function of ARFs in salt stress responses and offer a potential target for CRISPR/Cas-mediated salt-tolerant wheat breeding programs.
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Claims 15
Claim · model-written summary
TaARF18 was identified as a negative regulator of wheat salt tolerance.
- Taxon
- wheat
Triticum aestivume0 - Gene
- TaARF18e0
- Outcome
- salt tolerancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified TaARF18 as a negative regulator of salt tolerance in wheat”
Claim · model-written summary
TaARF18-A coding sequence was 2106 bp.
Not reportedTaxon
Verbatim
e0“The coding sequences of TaARF18-A, TaARF18-B, and TaARF18-D were 2106, 2088, and 2088 bp, respectively”
Claim · model-written summary
TaARF18-B coding sequence was 2088 bp.
Not reportedTaxon
Verbatim
e0“The coding sequences of TaARF18-A, TaARF18-B, and TaARF18-D were 2106, 2088, and 2088 bp, respectively”
Claim · model-written summary
TaARF18-D coding sequence was 2088 bp.
Not reportedTaxon
Verbatim
e0“The coding sequences of TaARF18-A, TaARF18-B, and TaARF18-D were 2106, 2088, and 2088 bp, respectively”
Claim · model-written summary
TaARF18 was hydrophilic and contained Auxin-resp and B3 DNA-binding domains.
Not reportedTaxon
Verbatim
e0“TaARF18 is a hydrophilic protein featuring typical Auxin-resp and B3 DNA-binding domains”
Claim · model-written summary
TaARF18 was relatively conserved among Poaceae species.
- Outcome
- relatively high evolutionary conservatione0
Verbatim
e0“exhibits relatively high evolutionary conservation among Poaceae species”
Claim · model-written summary
Salt stress upregulated TaARF18 expression.
Not reportedTaxonComparator
Verbatim
e0“The expression of TaARF18 was upregulated under salt stress”
Claim · model-written summary
TaARF18 predominantly accumulated in the nucleus.
Not reportedTaxon
Verbatim
e0“TaARF18 predominantly accumulated in the nucleus”
Claim · model-written summary
Silencing TaARF18 enhanced salt tolerance in wheat seedlings.
- Taxon
- wheatTriticum aestivume0
- Organ / stage
- seedlingse0
- Treatment
- Silencing of TaARF18 via the BSMV-VIGS approache0
- Gene
- TaARF18e0
- Outcome
- salt tolerancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of TaARF18 via the BSMV-VIGS approach enhanced salt tolerance in wheat seedlings”
Claim · model-written summary
TaARF18-A had 25 haplotypes among 355 wheat accessions.
- Taxon
- wheatTriticum aestivume0
- Gene
- TaARF18-Ae0
- Outcome
- haplotypese0
- Value
- 25e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“355 wheat accessions identified 25, 31, and 16 haplotypes for TaARF18-A, TaARF18-B, and TaARF18-D, respectively”
Claim · model-written summary
TaARF18-B had 31 haplotypes among 355 wheat accessions.
- Taxon
- wheatTriticum aestivume0
- Gene
- TaARF18-Be0
- Outcome
- haplotypese0
- Value
- 31e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“355 wheat accessions identified 25, 31, and 16 haplotypes for TaARF18-A, TaARF18-B, and TaARF18-D, respectively”
Claim · model-written summary
TaARF18-D had 16 haplotypes among 355 wheat accessions.
- Taxon
- wheatTriticum aestivume0
- Gene
- TaARF18-De0
- Outcome
- haplotypese0
- Value
- 16e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“355 wheat accessions identified 25, 31, and 16 haplotypes for TaARF18-A, TaARF18-B, and TaARF18-D, respectively”
Claim · model-written summary
HapIII of TaARF18-A had the highest salt tolerance among evaluated accessions.
Not reportedTaxon
Verbatim
e0“HapIII of TaARF18-A exhibited the highest level of salt tolerance”
Claim · model-written summary
The authors propose TaARF18-A HapIII as a wheat breeding selection locus.
- Taxon
- wheatTriticum aestivume0
- Material
- HapIIIe0
- Gene
- TaARF18-Ae0
- Outcome
- strong selection locuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“HapIII of TaARF18-A exhibited the highest level of salt tolerance, which can act as a strong selection locus in global wheat breeding”
Claim · model-written summary
The authors propose TaARF18 as a potential CRISPR/Cas target for salt-tolerant wheat breeding.
- Taxon
- wheatTriticum aestivume0
- Outcome
- potential target for CRISPR/Cas-mediated salt-tolerant wheat breeding programse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“offer a potential target for CRISPR/Cas-mediated salt-tolerant wheat breeding programs”
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See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.