Abstract
• PoSPX1 promotes root elongation and anthocyanin accumulation in tree peony under phosphate deficiency. • The organic phosphate transporter PoGlpT is strongly induced by Pi starvation in tree peony roots. • Overexpression of PoSPX1 enhances low-phosphorus tolerance in Arabidopsis by improving root and shoot growth. • These findings may offer preliminary genetic clues for improving phosphorus efficiency in tree peony cultivation. Tree peony ( Paeonia ostii ‘Fengdan’) is an economically important medicinal plant. The bark of its root, which is commonly known as Danpi, is rich in bioactive compounds. Phosphorus is essential for plant growth, and its availability can constrain plant development and metabolism. In this study, we examined the phenotypic, physiological, and molecular responses of ‘Fengdan’ seedlings to a gradient of phosphate (Pi) concentrations: deficiency (P0, 0 mM), sufficiency (P1, 1.25 mM), and high (P2, 2.00 mM). Pi deficiency reduced leaf area and root biomass initially, followed by a significant increase in both total and lateral root lengths and an elevated leaf anthocyanin content at a later stage. Transcriptomic analysis revealed numerous differentially expressed genes (DEGs) associated with Pi starvation response, transport, and metabolism. Five key candidate genes, PoSPX1, PoPHT1;7, PoPAP8, PoPAP22 , and PoGlpT were identified and their expression patterns were validated. PoSPX1 showed the highest overall responsiveness. PoGlpT , an organic phosphorus transporter poorly characterized in plants, was also highly responsive to Pi starvation. Notably, overexpression of PoSPX1 in Arabidopsis promoted root and shoot growth and stimulated anthocyanin accumulation under low-Pi conditions. These results suggest the adaptive mechanisms to phosphate deficiency in tree peony and indicate that PoSPX1 may play an important regulatory role. It may also provide insights for optimizing phosphorus fertilization strategies to improve Danpi yield and quality.
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Claims 11
Claim · model-written summary
Phosphate deficiency initially reduced leaf area and root biomass in tree peony seedlings.
- Taxon
- tree peony
Paeonia suffruticosat0 - Treatment
- Pi deficiencye1
- Outcome
- leaf area and root biomasse1
- Direction
- reducede1
- Qualifier
- initiallye1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Pi deficiency reduced leaf area and root biomass initially”
t0“tree peony”
Claim · model-written summary
Phosphate deficiency later significantly increased total and lateral root lengths in tree peony seedlings.
- Taxon
- tree peonyPaeonia suffruticosat0
- Treatment
- Pi deficiencye1
- Outcome
- total and lateral root lengthse1
- Direction
- increasee1
- Qualifier
- at a later stagee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Pi deficiency reduced leaf area and root biomass initially, followed by a significant increase in both total and lateral root lengths and an elevated leaf anthocyanin content at a later stage”
t0“tree peony”
Claim · model-written summary
Phosphate deficiency later elevated leaf anthocyanin content in tree peony seedlings.
- Taxon
- tree peonyPaeonia suffruticosat0
- Treatment
- Pi deficiencye1
- Outcome
- leaf anthocyanin contente1
- Direction
- elevatede1
- Qualifier
- at a later stagee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Pi deficiency reduced leaf area and root biomass initially, followed by a significant increase in both total and lateral root lengths and an elevated leaf anthocyanin content at a later stage”
t0“tree peony”
Claim · model-written summary
Transcriptomic analysis of tree peony under phosphate treatments revealed numerous differentially expressed genes associated with Pi starvation response, transport and metabolism.
- Taxon
- tree peonyPaeonia suffruticosat0
- Outcome
- numerous differentially expressed genes (DEGs) associated with Pi starvation response, transport, and metabolisme1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Transcriptomic analysis revealed numerous differentially expressed genes (DEGs) associated with Pi starvation response, transport, and metabolism”
t0“tree peony”
Claim · model-written summary
Five key candidate genes, PoSPX1, PoPHT1;7, PoPAP8, PoPAP22 and PoGlpT, were identified in tree peony and their expression patterns validated.
- Taxon
- tree peonyPaeonia suffruticosat0
- Gene
- PoSPX1, PoPHT1;7, PoPAP8, PoPAP22 , and PoGlpTe1
- Outcome
- Five key candidate genese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Five key candidate genes, PoSPX1, PoPHT1;7, PoPAP8, PoPAP22 , and PoGlpT were identified and their expression patterns were validated”
t0“tree peony”
Claim · model-written summary
Among the candidate genes, PoSPX1 showed the highest overall responsiveness.
- Taxon
- tree peonyPaeonia suffruticosat0
- Gene
- PoSPX1e1
- Outcome
- highest overall responsivenesse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“PoSPX1 showed the highest overall responsiveness”
t0“tree peony”
Claim · model-written summary
PoGlpT, an organic phosphorus transporter, was highly responsive to Pi starvation in tree peony.
- Taxon
- tree peonyPaeonia suffruticosat0
- Gene
- PoGlpTe1
- Outcome
- highly responsive to Pi starvatione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“PoGlpT , an organic phosphorus transporter poorly characterized in plants, was also highly responsive to Pi starvation”
t0“tree peony”
Claim · model-written summary
Overexpression of PoSPX1 in Arabidopsis promoted root and shoot growth under low-Pi conditions.
- Taxon
- Arabidopsis
Arabidopsise1 - Treatment
- overexpression of PoSPX1e1
- Gene
- PoSPX1e1
- Outcome
- root and shoot growthe1
- Direction
- promotede1
- Qualifier
- low-Pi conditionse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“overexpression of PoSPX1 in Arabidopsis promoted root and shoot growth and stimulated anthocyanin accumulation under low-Pi conditions”
Claim · model-written summary
Overexpression of PoSPX1 in Arabidopsis stimulated anthocyanin accumulation under low-Pi conditions.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- overexpression of PoSPX1e1
- Gene
- PoSPX1e1
- Outcome
- anthocyanin accumulatione1
- Direction
- stimulatede1
- Qualifier
- low-Pi conditionse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“overexpression of PoSPX1 in Arabidopsis promoted root and shoot growth and stimulated anthocyanin accumulation under low-Pi conditions”
Claim · model-written summary
PoSPX1 promotes root elongation and anthocyanin accumulation in tree peony under phosphate deficiency.
- Taxon
- tree peonyPaeonia suffruticosae1
- Treatment
- phosphate deficiencye1
- Gene
- PoSPX1e1
- Outcome
- root elongation and anthocyanin accumulatione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“PoSPX1 promotes root elongation and anthocyanin accumulation in tree peony under phosphate deficiency”
Claim · model-written summary
The authors suggest these results indicate adaptive mechanisms to phosphate deficiency in tree peony, with PoSPX1 possibly playing an important regulatory role.
- Taxon
- tree peonyPaeonia suffruticosae1
- Gene
- PoSPX1e1
- Outcome
- adaptive mechanisms to phosphate deficiencye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“These results suggest the adaptive mechanisms to phosphate deficiency in tree peony and indicate that PoSPX1 may play an important regulatory role”