Abstract
Roselle (Hibiscus sabdariffa) leaves are the major by-product after harvesting the commercial calyces, and they contain diverse functional metabolites such as phenolic acids and flavonoids. This study aimed to comprehensively characterize the metabolite profiles of the leaves of two Taiwanese roselle varieties (Taitung No. 6 and Taitung No. 3) and to evaluate their antiproliferative and insulin resistance-ameliorating activities. Extracts prepared with 50% EtOH and n-hexane were analyzed by untargeted and targeted LC-MS/MS and GC-MS, respectively. Results revealed that the 50% EtOH extracts of both varieties were rich in neochlorogenic acid, cryptochlorogenic acid, rutin, and nicotiflorin. Taitung No. 6 uniquely contained higher levels of anthocyanins including delphinidin-3-O-sambubioside and cyanidin-3-O-sambubioside. Acidified 50% EtOH extraction increased flavonoid aglycone and polyamine contents. n-Hexane extracts were identified as potential sources of vitamin E, α-linolenic acid, squalene, and phytol. Both varieties exhibited antiproliferative activity against BxPC-3 pancreatic cancer cells (IC50: 289.2–356.8 μg/mL) and significantly improved the glucose uptake in insulin-resistant HepG2 cells. Neochlorogenic acid and cryptochlorogenic acid were identified as primary active contributors to these bioactivities. This study elucidated the metabolite composition of Taiwanese roselle leaves and provided a scientific basis for the utilization of roselle leaves as a promising functional ingredient.
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Claims 9
Claim · model-written summary
The 50% EtOH leaf extracts of both roselle varieties were rich in neochlorogenic acid and cryptochlorogenic acid.
- Taxon
- Hibiscus sabdariffa
Hibiscus sabdariffat0 - Outcome
- rich in neochlorogenic acid, cryptochlorogenic acide0
- Qualifier
- 50% EtOH extracts of both varietiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Hibiscus sabdariffa”
e0“the 50% EtOH extracts of both varieties were rich in neochlorogenic acid, cryptochlorogenic acid, rutin, and nicotiflorin”
Claim · model-written summary
The 50% EtOH leaf extracts of both roselle varieties were rich in rutin and nicotiflorin.
- Taxon
- Hibiscus sabdariffaHibiscus sabdariffat0
- Outcome
- rutin, and nicotiflorine0
- Qualifier
- 50% EtOH extracts of both varietiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Hibiscus sabdariffa”
e0“the 50% EtOH extracts of both varieties were rich in neochlorogenic acid, cryptochlorogenic acid, rutin, and nicotiflorin”
Claim · model-written summary
Taitung No. 6 leaves uniquely contained higher levels of anthocyanins, including delphinidin-3-O-sambubioside and cyanidin-3-O-sambubioside.
- Taxon
- Hibiscus sabdariffaHibiscus sabdariffat0
- Material
- Taitung No. 6e0
- Outcome
- anthocyaninse0
- Direction
- highere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Hibiscus sabdariffa”
e0“Taitung No. 6 uniquely contained higher levels of anthocyanins including delphinidin-3-O-sambubioside and cyanidin-3-O-sambubioside”
Claim · model-written summary
Acidified 50% EtOH extraction increased flavonoid aglycone and polyamine contents of roselle leaf extracts.
- Taxon
- Hibiscus sabdariffaHibiscus sabdariffat0
- Treatment
- Acidified 50% EtOH extractione0
- Outcome
- flavonoid aglycone and polyamine contentse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Hibiscus sabdariffa”
e0“Acidified 50% EtOH extraction increased flavonoid aglycone and polyamine contents”
Claim · model-written summary
n-Hexane extracts of roselle leaves were identified as potential sources of vitamin E and alpha-linolenic acid.
- Taxon
- Hibiscus sabdariffaHibiscus sabdariffat0
- Outcome
- vitamin E, α-linolenic acide0
- Qualifier
- n-Hexane extractse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Hibiscus sabdariffa”
e0“n-Hexane extracts were identified as potential sources of vitamin E, α-linolenic acid, squalene, and phytol”
Claim · model-written summary
n-Hexane extracts of roselle leaves were identified as potential sources of squalene and phytol.
- Taxon
- Hibiscus sabdariffaHibiscus sabdariffat0
- Outcome
- squalene, and phytole0
- Qualifier
- n-Hexane extractse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Hibiscus sabdariffa”
e0“n-Hexane extracts were identified as potential sources of vitamin E, α-linolenic acid, squalene, and phytol”
Claim · model-written summary
Both roselle varieties showed antiproliferative activity against BxPC-3 pancreatic cancer cells with IC50 of 289.2-356.8 ug/mL.
- Taxon
- Hibiscus sabdariffaHibiscus sabdariffat0
- Outcome
- antiproliferative activity against BxPC-3 pancreatic cancer cellse0
- Value
- 289.2–356.8 μg/mLe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Hibiscus sabdariffa”
e0“Both varieties exhibited antiproliferative activity against BxPC-3 pancreatic cancer cells (IC50: 289.2–356.8 μg/mL)”
Claim · model-written summary
Both roselle varieties significantly improved glucose uptake in insulin-resistant HepG2 cells.
- Taxon
- Hibiscus sabdariffaHibiscus sabdariffat0
- Outcome
- glucose uptake in insulin-resistant HepG2 cellse0
- Direction
- improvede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Hibiscus sabdariffa”
e0“significantly improved the glucose uptake in insulin-resistant HepG2 cells”
Claim · model-written summary
Neochlorogenic acid and cryptochlorogenic acid were identified as the primary active contributors to the antiproliferative and glucose-uptake bioactivities.
- Taxon
- Hibiscus sabdariffaHibiscus sabdariffat0
- Compound
- Neochlorogenic acid and cryptochlorogenic acide0
- Outcome
- these bioactivitiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Hibiscus sabdariffa”
e0“Neochlorogenic acid and cryptochlorogenic acid were identified as primary active contributors to these bioactivities”
The same questions in other papers
ethanol · quality: phytochemicals · Hibiscus sabdariffa (roselle)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.