Abstract
BACKGROUND: The 14-3-3 proteins, also known as General Regulatory Factors (GRFs), are a family of highly conserved acidic proteins that mediate cellular processes and plant stress adaptation through protein-protein interactions. In this study, 9 GRF genes (ThGRF1-ThGRF9) were isolated and characterized from the halophytic species Tamarix hispida. RESULTS: qRT-PCR analyses showed that all nine ThGRFs responded to multiple stress response pathways. In particular, the expression levels of ThGRF4 and ThGRF5 were significantly upregulated in T. hispida roots under salt stress, suggesting their potential involvement in salt stress responses. Furthermore, an overexpression vector of ThGRF5 was constructed and transiently transformed into T. hispida. Biochemical staining and physiological parameter analyses showed that overexpression of ThGRF5 increased antioxidant enzyme activity, decreased MDA content and electrolyte leakage by decreasing the accumulation of ROS, thereby improving tolerance to salt stress. CONCLUSIONS: These results suggested that ThGRF5 might play an important physiological role in salt tolerance in transgenic T. hispida plants. This study provides a foundation for further elucidation of salt tolerance mechanisms involving ThGRF5 in T. hispida.
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Claims 9
Claim · model-written summary
Nine GRF genes were isolated and characterized from Tamarix hispida.
- Taxon
- Tamarix hispida
Tamarix hispidae0 - Gene
- ThGRF1-ThGRF9e0
- Outcome
- GRF genese0
- Value
- 9 GRF genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“9 GRF genes (ThGRF1-ThGRF9) were isolated and characterized from the halophytic species Tamarix hispida.”
Claim · model-written summary
All nine ThGRFs responded to multiple stress pathways.
- Taxon
- Tamarix hispidaTamarix hispidat0
- Gene
- ThGRFse0
- Outcome
- responded to multiple stress response pathwayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“all nine ThGRFs responded to multiple stress response pathways.”
t0“Tamarix hispida”
Claim · model-written summary
Salt stress significantly upregulated ThGRF4 expression in roots.
- Taxon
- T. hispidaTamarix hispidae0
- Organ / stage
- rootse0
- Treatment
- salt stresse0
- Gene
- ThGRF4e0
- Outcome
- expression levelse0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression levels of ThGRF4 and ThGRF5 were significantly upregulated in T. hispida roots under salt stress”
Claim · model-written summary
Salt stress significantly upregulated ThGRF5 expression in roots.
- Taxon
- T. hispidaTamarix hispidae0
- Organ / stage
- rootse0
- Treatment
- salt stresse0
- Gene
- ThGRF5e0
- Outcome
- expression levelse0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression levels of ThGRF4 and ThGRF5 were significantly upregulated in T. hispida roots under salt stress”
Claim · model-written summary
ThGRF5 overexpression increased antioxidant enzyme activity.
- Taxon
- Tamarix hispidaTamarix hispidat0
- Treatment
- overexpression of ThGRF5e0
- Gene
- ThGRF5e0
- Outcome
- antioxidant enzyme activitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of ThGRF5 increased antioxidant enzyme activity, decreased MDA content and electrolyte leakage by decreasing the accumulation of ROS, thereby improving tolerance to salt stress.”
t0“Tamarix hispida”
Claim · model-written summary
ThGRF5 overexpression decreased MDA content.
- Taxon
- Tamarix hispidaTamarix hispidat0
- Treatment
- overexpression of ThGRF5e0
- Gene
- ThGRF5e0
- Outcome
- MDA contente0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of ThGRF5 increased antioxidant enzyme activity, decreased MDA content and electrolyte leakage by decreasing the accumulation of ROS, thereby improving tolerance to salt stress.”
t0“Tamarix hispida”
Claim · model-written summary
ThGRF5 overexpression decreased electrolyte leakage.
- Taxon
- Tamarix hispidaTamarix hispidat0
- Treatment
- overexpression of ThGRF5e0
- Gene
- ThGRF5e0
- Outcome
- electrolyte leakagee0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of ThGRF5 increased antioxidant enzyme activity, decreased MDA content and electrolyte leakage by decreasing the accumulation of ROS, thereby improving tolerance to salt stress.”
t0“Tamarix hispida”
Claim · model-written summary
ThGRF5 overexpression decreased ROS accumulation.
- Taxon
- Tamarix hispidaTamarix hispidat0
- Gene
- ThGRF5t0
- Outcome
- accumulation of ROSe0
- Direction
- decreasinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“by decreasing the accumulation of ROS, thereby improving tolerance to salt stress.”
t0“ThGRF5, a 14–3-3 protein, acts as a positive regulator of salt tolerance in Tamarix hispida”
Claim · model-written summary
ThGRF5 overexpression improved salt tolerance.
- Taxon
- Tamarix hispidaTamarix hispidat0
- Treatment
- overexpression of ThGRF5e0
- Gene
- ThGRF5e0
- Outcome
- tolerance to salt stresse0
- Direction
- improvinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of ThGRF5 increased antioxidant enzyme activity, decreased MDA content and electrolyte leakage by decreasing the accumulation of ROS, thereby improving tolerance to salt stress.”
t0“Tamarix hispida”
The same questions in other papers
salinity · molecular: expression · Tamarix hispidaResults agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.