Paper · 1997 · TURKISH JOURNAL OF BIOLOGY

Determination of some Biochemical Properties of Wheat (Triticum aestivum L.) \alpha-Amylase Enzyme and Effects of Endosulfan on Enzyme Activity

Filiz EKİNCİ; Tülin AKTAÇ

7
claims
13
verbatim quotes
4
citations
1
research areas
—
Not open access

Abstract

In this study, some of the biochemical properties of \alpha-amylase enzyme, which plays an important role in seed germination were determined in wheat grain. The effects of endosulfan on enzyme activity of \alpha-amylase and the total protein amount of wheat grain were also investigated. Wheat (Triticum aestivum Pehlivan) \alpha-amylase enzyme activity was obtained at highest Ievel between 2nd and 5th days of germination. The optimum conditions for this enzyme were determinated at pH: 5.2, incubation temparature. 30°C, incubation period: 3 minutes. It was also determinated that the Iiquid and powder forms of endusulfan, depending on dose, inhibited the enzyme activity and caused decreases in the total amount of protein. The reason could be the inhibition effect of endosulfan on the enzyme synthesis of \alpha-amylase.

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Claims 7

Observationobservedoriginal resultmeasured

Claim · model-written summary

Wheat alpha-amylase activity peaked between days two and five of germination.

Material
Pehlivane0
Organ / stage
germinatione0
Gene
\alpha-amylasee0
Outcome
\alpha-amylase enzyme activitye0
Direction
highest Ievele0
Qualifier
between 2nd and 5th days of germinatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Wheat (Triticum aestivum Pehlivan) \alpha-amylase enzyme activity was obtained at highest Ievel between 2nd and 5th days of germination.”

✓ located verbatim in the source · abstract, characters 289–425 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The reported optimum for wheat alpha-amylase was pH 5.2, 30°C and three minutes of incubation.

Gene
enzymee0
Outcome
optimum conditionse0
Value
pH: 5.2, incubation temparature. 30°C, incubation period: 3 minutese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The optimum conditions for this enzyme were determinated at pH: 5.2, incubation temparature. 30°C, incubation period: 3 minutes.”

✓ located verbatim in the source · abstract, characters 426–554 · show in text ↑
t0

“Wheat (Triticum aestivum L.)”

✓ located verbatim in the source · title, characters 48–76 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Liquid endosulfan inhibited wheat alpha-amylase activity in a dose-dependent manner.

Treatment
Iiquide0
Outcome
enzyme activitye0
Direction
inhibitede0
Qualifier
depending on dosee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the Iiquid and powder forms of endusulfan, depending on dose, inhibited the enzyme activity and caused decreases in the total amount of protein”

✓ located verbatim in the source · abstract, characters 585–728 · show in text ↑
t0

“Wheat (Triticum aestivum L.)”

✓ located verbatim in the source · title, characters 48–76 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Powder endosulfan inhibited wheat alpha-amylase activity in a dose-dependent manner.

Treatment
powder forms of endusulfane0
Outcome
enzyme activitye0
Direction
inhibitede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the Iiquid and powder forms of endusulfan, depending on dose, inhibited the enzyme activity and caused decreases in the total amount of protein”

✓ located verbatim in the source · abstract, characters 585–728 · show in text ↑
t0

“Wheat (Triticum aestivum L.)”

✓ located verbatim in the source · title, characters 48–76 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Liquid endosulfan decreased total protein in wheat grain.

Treatment
Iiquide0
Outcome
total amount of proteine0
Direction
decreasese0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the Iiquid and powder forms of endusulfan, depending on dose, inhibited the enzyme activity and caused decreases in the total amount of protein”

✓ located verbatim in the source · abstract, characters 585–728 · show in text ↑
t0

“Wheat (Triticum aestivum L.)”

✓ located verbatim in the source · title, characters 48–76 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Powder endosulfan decreased total protein in wheat grain.

Treatment
powder forms of endusulfane0
Outcome
total amount of proteine0
Direction
decreasese0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the Iiquid and powder forms of endusulfan, depending on dose, inhibited the enzyme activity and caused decreases in the total amount of protein”

✓ located verbatim in the source · abstract, characters 585–728 · show in text ↑
t0

“Wheat (Triticum aestivum L.)”

✓ located verbatim in the source · title, characters 48–76 · show in text ↑
Mechanismhypothesisoriginal resultbasis n/a

Claim · model-written summary

The authors suggest endosulfan may inhibit alpha-amylase synthesis.

Treatment
endosulfane0
Gene
\alpha-amylasee0
Outcome
enzyme synthesis of \alpha-amylasee0
Qualifier
could bee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The reason could be the inhibition effect of endosulfan on the enzyme synthesis of \alpha-amylase.”

✓ located verbatim in the source · abstract, characters 730–828 · show in text ↑
t0

“Wheat (Triticum aestivum L.)”

✓ located verbatim in the source · title, characters 48–76 · show in text ↑

botanical · soil: biology · Triticum aestivum (wheat)Positive and negative results

4 papers: 3 positive, 1 negative or no effect

3 in the opposite direction

  1. positive
  2. positive
  3. positive
See all 4 papers →

botanical · quality: protein · Triticum aestivum (wheat)Positive and negative results

24 papers: 17 positive, 7 negative or no effect

6 papers in the same direction as this paper · 17 in the opposite direction

  1. positive
  2. positive
  3. positive

20 papers more in this group

See all 24 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.