Abstract
BackgroundHumic acid or chitosan has been shown to increase plant growth, yield and improving physiological processes in plant, but its roles on alleviating the harmful effect of cadmium on plant growth and some physiological processes in plants is very rare. Pot experiments were conducted to study the role of 100 and 200 mg/kg dry soil from either humic acid or chitosan on counteracted the harmful effects of cadmium levels (100 and 150 mg/kg dry soil) on radish plant growth and some physiological charactersResultsCadmium at 100 and 150 mg kg-1 soil decreased significantly length, fresh and dry weights of shoot and root systems as well as leaf number per plant in both seasons. Chlorophyll, total sugars, nitrogen, phosphorus, potassium, relative water content, water deficit percentage and soluble proteins as well as total amino acids contents were also decreased. Meanwhile, cadmium concentration in plants was increased. On the other hand, application of chitosan or humic acid as soil addition at the concentration of 100 or 200 mg kg-1 increased all the above mentioned parameters and decreased cadmium concentrations in plant tissues. Chitosan at 200 mg kg-1 was the most effective than humic acid at both concentrations in counteracting the harmful effect of cadmium stress on radish plant growth.ConclusionIn conclusion, both natural chelators, in particular, chitosan at 200 mg/kg dry soil can increase the capacity of radish plant to survive under cadmium stress due to chelating the Cd in the soil, and then reduced Cd bio-availability.
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Claims 11
Claim · model-written summary
Cadmium at 100 and 150 mg/kg soil significantly decreased shoot and root length, fresh and dry weights, and leaf number in radish.
- Taxon
- radish
Raphanus sativuse0 - Treatment
- Cadmium at 100 and 150 mg kg-1 soile1
- Outcome
- length, fresh and dry weights of shoot and root systemse1
- Direction
- decreasede1
- Qualifier
- in both seasonse1
- Setting
- Pot experimentse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Pot experiments were conducted to study the role of 100 and 200 mg/kg dry soil from either humic acid or chitosan on counteracted the harmful effects of cadmium levels (100 and 150 mg/kg dry soil) on radish plant growth”
e1“Cadmium at 100 and 150 mg kg-1 soil decreased significantly length, fresh and dry weights of shoot and root systems as well as leaf number per plant in both seasons”
Claim · model-written summary
Cadmium at 100 and 150 mg/kg soil significantly decreased leaf number per radish plant.
- Taxon
- radish
Raphanus sativuse0 - Treatment
- Cadmium at 100 and 150 mg kg-1 soile1
- Outcome
- leaf number per plante1
- Direction
- decreasede1
- Qualifier
- in both seasonse1
- Setting
- Pot experimentse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Pot experiments were conducted to study the role of 100 and 200 mg/kg dry soil from either humic acid or chitosan on counteracted the harmful effects of cadmium levels (100 and 150 mg/kg dry soil) on radish plant growth”
e1“Cadmium at 100 and 150 mg kg-1 soil decreased significantly length, fresh and dry weights of shoot and root systems as well as leaf number per plant in both seasons”
Claim · model-written summary
Cadmium decreased chlorophyll, total sugars and nitrogen contents of radish.
- Taxon
- radish
Raphanus sativuse0 - Treatment
- Cadmium at 100 and 150 mg kg-1 soile1
- Outcome
- Chlorophyll, total sugars, nitrogene2
- Direction
- decreasede2
- Setting
- Pot experimentse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Pot experiments were conducted to study the role of 100 and 200 mg/kg dry soil from either humic acid or chitosan on counteracted the harmful effects of cadmium levels (100 and 150 mg/kg dry soil) on radish plant growth”
e1“Cadmium at 100 and 150 mg kg-1 soil decreased significantly length, fresh and dry weights of shoot and root systems as well as leaf number per plant in both seasons”
e2“Chlorophyll, total sugars, nitrogen, phosphorus, potassium, relative water content, water deficit percentage and soluble proteins as well as total amino acids contents were also decreased”
Claim · model-written summary
Cadmium decreased phosphorus, potassium and relative water content of radish.
- Taxon
- radish
Raphanus sativuse0 - Treatment
- Cadmium at 100 and 150 mg kg-1 soile1
- Outcome
- phosphorus, potassium, relative water contente2
- Direction
- decreasede2
- Setting
- Pot experimentse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Pot experiments were conducted to study the role of 100 and 200 mg/kg dry soil from either humic acid or chitosan on counteracted the harmful effects of cadmium levels (100 and 150 mg/kg dry soil) on radish plant growth”
e1“Cadmium at 100 and 150 mg kg-1 soil decreased significantly length, fresh and dry weights of shoot and root systems as well as leaf number per plant in both seasons”
e2“Chlorophyll, total sugars, nitrogen, phosphorus, potassium, relative water content, water deficit percentage and soluble proteins as well as total amino acids contents were also decreased”
Claim · model-written summary
Cadmium decreased water deficit percentage and soluble proteins of radish.
- Taxon
- radish
Raphanus sativuse0 - Treatment
- Cadmium at 100 and 150 mg kg-1 soile1
- Outcome
- water deficit percentage and soluble proteinse2
- Direction
- decreasede2
- Setting
- Pot experimentse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Pot experiments were conducted to study the role of 100 and 200 mg/kg dry soil from either humic acid or chitosan on counteracted the harmful effects of cadmium levels (100 and 150 mg/kg dry soil) on radish plant growth”
e1“Cadmium at 100 and 150 mg kg-1 soil decreased significantly length, fresh and dry weights of shoot and root systems as well as leaf number per plant in both seasons”
e2“Chlorophyll, total sugars, nitrogen, phosphorus, potassium, relative water content, water deficit percentage and soluble proteins as well as total amino acids contents were also decreased”
Claim · model-written summary
Cadmium decreased total amino acid contents of radish.
- Taxon
- radish
Raphanus sativuse0 - Treatment
- Cadmium at 100 and 150 mg kg-1 soile1
- Outcome
- total amino acids contentse2
- Direction
- decreasede2
- Setting
- Pot experimentse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Pot experiments were conducted to study the role of 100 and 200 mg/kg dry soil from either humic acid or chitosan on counteracted the harmful effects of cadmium levels (100 and 150 mg/kg dry soil) on radish plant growth”
e1“Cadmium at 100 and 150 mg kg-1 soil decreased significantly length, fresh and dry weights of shoot and root systems as well as leaf number per plant in both seasons”
e2“Chlorophyll, total sugars, nitrogen, phosphorus, potassium, relative water content, water deficit percentage and soluble proteins as well as total amino acids contents were also decreased”
Claim · model-written summary
Cadmium increased cadmium concentration in radish plants.
- Taxon
- radish
Raphanus sativuse0 - Treatment
- Cadmium at 100 and 150 mg kg-1 soile1
- Outcome
- cadmium concentration in plantse3
- Direction
- increasede3
- Setting
- Pot experimentse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Pot experiments were conducted to study the role of 100 and 200 mg/kg dry soil from either humic acid or chitosan on counteracted the harmful effects of cadmium levels (100 and 150 mg/kg dry soil) on radish plant growth”
e1“Cadmium at 100 and 150 mg kg-1 soil decreased significantly length, fresh and dry weights of shoot and root systems as well as leaf number per plant in both seasons”
e3“Meanwhile, cadmium concentration in plants was increased”
Claim · model-written summary
Chitosan or humic acid soil addition at 100 or 200 mg/kg increased all the above mentioned radish parameters.
- Taxon
- radish
Raphanus sativuse0 - Treatment
- chitosan or humic acid as soil addition at the concentration of 100 or 200 mg kg-1e4
- Outcome
- all the above mentioned parameterse4
- Direction
- increasede4
- Setting
- Pot experimentse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Pot experiments were conducted to study the role of 100 and 200 mg/kg dry soil from either humic acid or chitosan on counteracted the harmful effects of cadmium levels (100 and 150 mg/kg dry soil) on radish plant growth”
e4“application of chitosan or humic acid as soil addition at the concentration of 100 or 200 mg kg-1 increased all the above mentioned parameters and decreased cadmium concentrations in plant tissues”
Claim · model-written summary
Chitosan or humic acid soil addition at 100 or 200 mg/kg decreased cadmium concentrations in radish tissues.
- Taxon
- radish
Raphanus sativuse0 - Treatment
- chitosan or humic acid as soil addition at the concentration of 100 or 200 mg kg-1e4
- Outcome
- cadmium concentrations in plant tissuese4
- Direction
- decreasede4
- Setting
- Pot experimentse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Pot experiments were conducted to study the role of 100 and 200 mg/kg dry soil from either humic acid or chitosan on counteracted the harmful effects of cadmium levels (100 and 150 mg/kg dry soil) on radish plant growth”
e4“application of chitosan or humic acid as soil addition at the concentration of 100 or 200 mg kg-1 increased all the above mentioned parameters and decreased cadmium concentrations in plant tissues”
Claim · model-written summary
Chitosan at 200 mg/kg was the most effective treatment, more than humic acid at both concentrations, in counteracting cadmium stress on radish growth.
- Taxon
- radish
Raphanus sativuse5 - Treatment
- Chitosan at 200 mg kg-1e5
- Outcome
- counteracting the harmful effect of cadmium stress on radish plant growthe5
- Direction
- the most effectivee5
- Comparator
- humic acid at both concentrationse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e5“Chitosan at 200 mg kg-1 was the most effective than humic acid at both concentrations in counteracting the harmful effect of cadmium stress on radish plant growth”
Claim · model-written summary
Chitosan at 200 mg/kg is suggested to raise radish survival under cadmium stress by chelating soil Cd and reducing its bioavailability.
- Taxon
- radish plant
Raphanus sativuse6 - Treatment
- chitosan at 200 mg/kg dry soile6
- Outcome
- capacity of radish plant to survive under cadmium stresse6
- Qualifier
- chelating the Cd in the soile6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e6“both natural chelators, in particular, chitosan at 200 mg/kg dry soil can increase the capacity of radish plant to survive under cadmium stress due to chelating the Cd in the soil, and then reduced Cd bio-availability”
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