Search claims · PlantClaims

Observationobservedoriginal resultmeasured

Claim · model-written summary

PCR confirmed transfer of rol A, rol B and rol C genes into the Arnica genome.

Taxon: ArnicaArnicae0
Gene: rol A, rol B and rol Ce0
Outcome: transfer of rol A, rol B and rol C genes into Arnica genomee0

Not reportedDirection

Verbatim

e0
“The transfer of rol A, rol B and rol C genes into Arnica genome was confirmed by PCR analysis.”
✓ located verbatim in the source · abstract, characters 678–772

Title

Induction of hairy roots in Arnica montana L. by Agrobacterium rhizogenes

Mariya Petrova; Ely Zayova; Mariana Vlahova Open Life Sciences 2013. doi:10.2478/s11535-013-0157-6

Other claims from this paper (4)

Observation Genetic transformation frequency ranged from 4.8 to 12% depending on infection method.
Intervention effect Cefotaxime at 200 mg/l suppressed A. rhizogenes growth after co-cultivation.
Intervention effect MS medium was superior for transformed-root growth and biomass production compared with the other media.
Intervention effect After 40 days, clone T4 fresh weight increased 7.6 fold over non-transformed roots.

Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

Deoxygenation induced a root basal ROL barrier and changed the ROL profile.

Taxon: Wetland Plantst0
Organ / stage: root basal parte0
Outcome: ROL barriere0
Qualifier: consequent change of ROL profilee0

Verbatim

e0
“inducing a ROL barrier in root basal part and the consequent change of ROL profile”
✓ located verbatim in the source · abstract, characters 743–825

t0
“Wetland Plants”
✓ located verbatim in the source · title, characters 51–65

Title

Characteristics of Radial Oxygen Loss from Root of Wetland Plants

Ming-Hong Huang Huadong Shifan Daxue xuebao. Ziran kexue ban 2007

Other claims from this paper (5)

Observation Root radial oxygen loss varied with distance from root tips and species.
Observation One ROL pattern had high rates along the root, gradually declining from apex to base.
Observation A second ROL pattern had high apex oxygen loss that greatly decreased toward the base.
Observation A third ROL pattern remained very low along the whole root.
Intervention effect Deoxygenation increased root diameter and porosity and developed aerenchyma in some species.

Observationobservedreview summarybasis n/a

Claim · model-written summary

Experiments described Rol protein localization and enzymatic activity toward phytohormone glucosides.

Taxon: plante1
Gene: Rol proteinse0
Outcome: subcellular localization of Rol proteins and enzymatic activity of Rol proteinse0

Verbatim

e0
“Experimental evidence of subcellular localization of Rol proteins and enzymatic activity of Rol proteins with respect to phytohormone glucosides”
✓ located verbatim in the source · abstract, characters 1505–1649

e1
“plant genomes”
✓ located verbatim in the source · abstract, characters 148–161

Title

rol Genes of Agrobacteria: Possible Biological Functions

D. Yu. Shvets; Z. A. Berezhneva; Kh. G. Musin; et al. Biology Bulletin Reviews 2023. doi:10.1134/s2079086423090116

Other claims from this paper (5)

Observationobservedoriginal resultmeasured

Claim · model-written summary

The tltl root culture carried rol C and rol D inserts.

Taxon: Pisum sativum L.Lathyrus oleraceust0
Material: genotypetltle0
Outcome: two —rol Candrol D— in the genomee0

Verbatim

e0
“two —rol Candrol D— in the genome of the root culture with genotypetltl.”
✓ located verbatim in the source · abstract, characters 549–621

t0
“Pisum sativum L.”
✓ located verbatim in the source · title, characters 55–71

Title

Some biochemical characteristics of the hairy roots of Pisum sativum L. mutants

Olga Timina; Oleg Timin; Anna Stepanova Ecological genetics 2023. doi:10.17816/ecogen568310

Other claims from this paper (6)

Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Amplification of rol A and rol B confirmed that hairy roots and transformed callus were transgenic.

Taxon: Berberis aristata DC.Berberis aristatat0
Gene: rol A and rol B genese0
Outcome: transgenic nature of hairy roots and transformed calluse0

Not reportedDirection

Verbatim

e0
“The presence of rol A and rol B genes during amplification confirmed the transgenic nature of hairy roots and transformed callus”
✓ located verbatim in the source · abstract, characters 590–718

t0
“Berberis aristata DC.”
✓ located verbatim in the source · title, characters 69–90

Title

Agrobacterium rhizogenes mediated hairy root induction in endangered Berberis aristata DC.

Latika Brijwal; Sushma Tamta SpringerPlus 2015. doi:10.1186/s40064-015-1222-1

Other claims from this paper (4)

Intervention effect Agrobacterium strain 532 induced hairy roots more effectively than strain 2364.
Intervention effect In vitro grown callus gave 61.11 ± 1.60 % transformation frequency and was more suitable than leaves.
Intervention effect In vitro grown callus gave a higher transformation frequency than nodal segments, which gave 34.25 ± 0.92 %.
Intervention effect Acetosyringone during co-cultivation raised callus transformation frequency from 61.11 ± 1.60 % to 72.22 ± 1.60 %.

Observationobservedoriginal resultmeasured

Claim · model-written summary

PCR showed integration of rol A and rol C genes into the plant genome in hairy roots.

Taxon: Linum leoniiLinum leoniit0
Organ / stage: hairy rootse0
Gene: rol A and rol C genese0
Outcome: integration of rol A and rol C genes into the plant genomee0

Verbatim

e0
“The genetic transformation in hairy roots was proven by PCR analysis, which showed integration of rol A and rol C genes into the plant genome”
✓ located verbatim in the source · abstract, characters 65–206

t0
“Linum leonii”
✓ located verbatim in the source · title, characters 109–121

Title

Production of Justicidin B, a Cytotoxic Arylnaphthalene Lignan from Genetically Transformed Root Cultures of Linum leonii

Nikolay Vasilev; Elfahmi; Rein Bos; et al. Journal of Natural Products 2006. doi:10.1021/np060022k

Other claims from this paper (3)

Method / resource Callus and hairy root cultures of Linum leonii were established.
Observation Calli and hairy roots accumulated justicidin B as a major constituent.
Observation Hairy roots produced fivefold more justicidin B than calli, yielding 10.8 mg g(-1) DW.

Observationobservedoriginal resultmeasured

Claim · model-written summary

Transformation of hairy roots was established by PCR amplification of rol B and rol C genes.

Taxon: Gossypium hirsutumGossypium hirsutume0
Gene: rol B and rol C genese2
Outcome: transformation of hairy rootse2

Verbatim

e0
“Agrobacterium rhizogenes mediated hairy root culture of Gossypium hirsutum”
✓ located verbatim in the source · abstract, characters 46–120

e2
“The transformation of hairy roots was established by PCR amplification of rol B and rol C genes of the Ri plasmid”
✓ located verbatim in the source · abstract, characters 339–452

Title

Efficient Production of Gossypol from Hairy Root Cultures of Cotton (Gossypium hirsutum L.)

Praveen Verma; Ila Trivedi; Harpal Singh; et al. Current Pharmaceutical Biotechnology 2009. doi:10.2174/138920109789542048

Other claims from this paper (7)

Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Rol B and rol C genes were retained in the hairy roots.

Taxon: cauliflowerBrassica oleracea var. botrytise0
Organ / stage: hairy rootse0
Gene: rol B and rol Ce0
Outcome: hairy roots retention of rol B and rol C genese0

Verbatim

e0
“The current study demonstrated that A. rhizogenes strain (ATCC13332) is a vector for the incorporation of T-DNA genes into cauliflower plants, as well as the success of the hairy roots retention of rol B and rol C genes transferred to it”
✓ located verbatim in the source · abstract, characters 847–1084

Title

A high yield method for protoplast isolation and ease detection of rol B and C genes in the hairy roots of cauliflower (Brassica oleracea L.) inoculated with Agrobacterium rhizogenes

Qutaiba Shuaib Al-Nema; Ghazwan Qasim Hasan; Omar Abdulazeez Alhamd Karbala International Journal of Modern Science 2022. doi:10.33640/2405-609x.3255

Other claims from this paper (6)

Method / resource Protoplast isolation with 1.5% Cellulase YC and 0.1% Pectolyase Y23 gave a protoplast yield of 2 × 104 cells per ml.
Method / resource Protoplast isolation gave a viability of 72%.
Method / resource Protoplast isolation was highly efficient within 8 hours.
Observation Each protoplast had one nucleus, with a nucleation of 48%.
Observation PCR verified the presence of rol B and rol C genes in the hairy root tissues of cauliflower.
Observation Agrobacterium rhizogenes strain (ATCC13332) is a vector for incorporating T-DNA genes into cauliflower plants.

Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

The rapid decrease in subapical ROL indicates induction of a barrier to ROL (author interpretation).

Taxon: Oryza sativaOryza sativae0
Outcome: ROLe1
Qualifier: induction of a barrier to ROLe1

Verbatim

e0
“Rice (Oryza sativa 'Amaroo') was grown in aerated solution”
✓ located verbatim in the source · abstract, characters 378–436

e1
“Roots responded rapidly to oxygen deficiency with decreased ROL in subapical zones within 1-2 d, indicating induction of a barrier to ROL”
✓ located verbatim in the source · abstract, characters 1351–1488

Title

Morphological and Physiological Responses of Rice (Oryza sativa) to Limited Phosphorus Supply in Aerated and Stagnant Solution Culture

N. INSALUD; R. W. BELL; T. D. COLMER; et al. Annals of Botany 2006. doi:10.1093/aob/mcl194