Following fertilisation the Arabidopsis zygote divides asymmetrically to generate apicalandbasallineages, establishing the apical-basal axis that defines future shoot and root poles.
A detailed protocol isolates early Arabidopsis proembryos and separates their apicalandbasal cell lineages for lineage-specific transcriptomes, compared with a previously established tobacco protocol.
“This work also provides a valuable lineage-specific transcriptome resource to elucidate the molecular pathways for divergence of apicalandbasal cell lineages at genome-wide scale.”
not strictly synchronized between the apical and basal lineagese0
Verbatim
e0
“The following cell divisions are not strictly synchronized between the apicalandbasallineages, exhibiting non-stereotypic patterns up to the globular stage of embryogenesis.”
t0
“3D imaging reveals robustness and plasticity of cell division in rice early embryogenesis”
“In angiosperms, the first zygotic division usually gives rise to two daughter cells with distinct morphologies and developmental fates”
e2
“We also demonstrated that the isolated apical cell could develop into a small globular embryo, both in vivo and in vitro, after artificial dysfunction of the basal cell; however, these growing apical cell lineages could never generate a new suspensor”
“In angiosperms, the first zygotic division usually gives rise to two daughter cells with distinct morphologies and developmental fates”
e2
“We also demonstrated that the isolated apical cell could develop into a small globular embryo, both in vivo and in vitro, after artificial dysfunction of the basal cell; however, these growing apical cell lineages could never generate a new suspensor”
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Arabidopsist0
Oryza sativa