Claim · model-written summary
The primer and probe sequences were specific to F. graminearum s. str. and F. asiaticum.
- Taxon
- wheat
Triticum aestivumt0 - Interactor
- F. graminearum s. str.
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=60)
Fusarium graminearume4pathogen - Second interactor
- F. asiaticum
Fusarium asiaticume4pathogen - Outcome
- primer and probe sequencese4
- Qualifier
- specific to F. graminearum s. str. and F. asiaticume4
Not reportedMaterialGeneCompoundOrgan / stageTreatmentComparatorSettingDirectionValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=500)
Verbatim
t0“Quantitative Real-Time PCR for Fusarium graminearum sensu stricto and F. asiaticum Infection in Wheat Kernels”
e4“In particular, the primer and probe sequences prepared in this study were specific to F. graminearum s. str. and F. asiaticum, making the TaqMan assay suitable for quantifying infections by these species”
Title
Yohei Yamagami; Yuhei Kanazashi; Hiroyoshi Inoue; et al. JSM Mycotoxins 2026. doi:10.2520/myco.76-1-2
Other claims from this paper (4)
- Method / resource Copy numbers of the target gene obtained from the two real-time PCR assays were strongly correlated in bulk samples (r = 0.97).
- Association EF1α gene copy number from the SYBR Green assay was correlated with ergosterol content of the wheat kernels (r = 0.88).
- Method / resource Real-time PCR provides a reliable method for quantifying F. graminearum s. str. and F. asiaticum infection.
- Method / resource The TaqMan assay is suitable for quantifying infections by these Fusarium species.