Claim · model-written summary
EF1α gene copy number from the SYBR Green assay was correlated with ergosterol content of the wheat kernels (r = 0.88).
- Taxon
- wheat
Triticum aestivume1 - Interactor
- F. graminearum s. str.
Fusarium graminearume1pathogen - Gene
- EF1α genee2
- Outcome
- ergosterol contente2
- Value
- r = 0.88e2
Not reportedMaterialCompoundOrgan / stageSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

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Verbatim
t0“Quantitative Real-Time PCR for Fusarium graminearum sensu stricto and F. asiaticum Infection in Wheat Kernels”
e1“Total DNA was extracted from wheat kernels harvested from a field where F. graminearum s. str. and F. asiaticum infection in wheat was artificially promoted”
e2“as well as between the EF1α gene copy number (SYBR Green assay) and ergosterol content (r = 0.88)”
Title
Yohei Yamagami; Yuhei Kanazashi; Hiroyoshi Inoue; et al. JSM Mycotoxins 2026. doi:10.2520/myco.76-1-2
Other claims from this paper (4)
- Method / resource Copy numbers of the target gene obtained from the two real-time PCR assays were strongly correlated in bulk samples (r = 0.97).
- Method / resource Real-time PCR provides a reliable method for quantifying F. graminearum s. str. and F. asiaticum infection.
- Method / resource The primer and probe sequences were specific to F. graminearum s. str. and F. asiaticum.
- Method / resource The TaqMan assay is suitable for quantifying infections by these Fusarium species.