Claim · model-written summary
Real-time PCR provides a reliable method for quantifying F. graminearum s. str. and F. asiaticum infection.
- Taxon
- wheat
Triticum aestivume1 - Interactor
- F. graminearum s. str.
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=60)
Fusarium graminearume3pathogen - Second interactor
- F. asiaticum
Fusarium asiaticume3pathogen - Outcome
- quantifying F. graminearum s. str. and F. asiaticum infectione3
Not reportedMaterialGeneCompoundOrgan / stageTreatmentComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=500)
Verbatim
t0“Quantitative Real-Time PCR for Fusarium graminearum sensu stricto and F. asiaticum Infection in Wheat Kernels”
e1“Total DNA was extracted from wheat kernels harvested from a field where F. graminearum s. str. and F. asiaticum infection in wheat was artificially promoted”
e3“These results indicate that real-time PCR provides a reliable method for quantifying F. graminearum s. str. and F. asiaticum infection”
Title
Yohei Yamagami; Yuhei Kanazashi; Hiroyoshi Inoue; et al. JSM Mycotoxins 2026. doi:10.2520/myco.76-1-2
Other claims from this paper (4)
- Method / resource Copy numbers of the target gene obtained from the two real-time PCR assays were strongly correlated in bulk samples (r = 0.97).
- Association EF1α gene copy number from the SYBR Green assay was correlated with ergosterol content of the wheat kernels (r = 0.88).
- Method / resource The primer and probe sequences were specific to F. graminearum s. str. and F. asiaticum.
- Method / resource The TaqMan assay is suitable for quantifying infections by these Fusarium species.