Claim · model-written summary
Copy numbers of the target gene obtained from the two real-time PCR assays were strongly correlated in bulk samples (r = 0.97).
- Taxon
- wheat
Triticum aestivume1 - Interactor
- F. graminearum s. str.
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=60)
Fusarium graminearume1pathogen - Second interactor
- F. asiaticum
Fusarium asiaticume1pathogen - Outcome
- copy numbers obtained from the two assayse0
- Value
- r = 0.97e0
- Setting
- a fielde1
Not reportedMaterialGeneCompoundOrgan / stageTreatmentComparatorDirectionQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=500)
Verbatim
t0“Quantitative Real-Time PCR for Fusarium graminearum sensu stricto and F. asiaticum Infection in Wheat Kernels”
e0“Strong correlations were observed between the copy numbers obtained from the two assays (r = 0.97 for bulk samples)”
e1“Total DNA was extracted from wheat kernels harvested from a field where F. graminearum s. str. and F. asiaticum infection in wheat was artificially promoted”
Title
Yohei Yamagami; Yuhei Kanazashi; Hiroyoshi Inoue; et al. JSM Mycotoxins 2026. doi:10.2520/myco.76-1-2
Other claims from this paper (4)
- Association EF1α gene copy number from the SYBR Green assay was correlated with ergosterol content of the wheat kernels (r = 0.88).
- Method / resource Real-time PCR provides a reliable method for quantifying F. graminearum s. str. and F. asiaticum infection.
- Method / resource The primer and probe sequences were specific to F. graminearum s. str. and F. asiaticum.
- Method / resource The TaqMan assay is suitable for quantifying infections by these Fusarium species.