By interacting organism › Viruses › Tymoviridae › Grapevine fleck virus

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

RT-ddPCR detected GFkV in 84.8% of field samples.

Taxon: GrapevineVitist0
Interactor: GFkVGrapevine fleck viruse0pathogen
Outcome: GFkV was detectede0
Value: 84.8% (56/66)e0
Comparator: RT-qPCRe0

Verbatim

t0
“in Grapevine”
✓ located verbatim in the source · title, characters 120–132

e0
“In field validation, GFkV was detected in 84.8% (56/66) of the samples via RT-ddPCR, whereas RT-qPCR detected the virus in 53.0% (35/66) of the samples.”
✓ located verbatim in the source · abstract, characters 763–915

Title

Digital Polymerase Chain Reaction as a Sensitive Diagnostic Tool: Detection and Quantification of Grapevine Fleck Virus in Grapevine

Min-Jae Kim; Hyo-Jeong Lee; Rae-Dong Jeong Research in Plant Disease 2026. doi:10.5423/rpd.2026.32.1.87

Other claims from this paper (6)

Method / resource The RT-ddPCR assay showed high specificity without cross-reactivity with other grapevine viruses.
Method / resource RT-ddPCR sensitivity was approximately tenfold higher than RT-qPCR.
Method / resource RT-ddPCR had a detection limit of 0.73 copies/μl.
Method / resource RT-ddPCR had high linearity, R2=0.9997.
Method / resource RT-qPCR had high linearity, R2=0.9983.
Method / resource RT-qPCR detected GFkV in 53.0% of field samples.

Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

RT-qPCR detected GFkV in 53.0% of field samples.

Taxon: GrapevineVitist0
Interactor: GFkVGrapevine fleck viruse0pathogen
Outcome: detected the viruse0
Value: 53.0% (35/66)e0
Comparator: RT-ddPCRe0

Verbatim

t0
“in Grapevine”
✓ located verbatim in the source · title, characters 120–132

e0
“In field validation, GFkV was detected in 84.8% (56/66) of the samples via RT-ddPCR, whereas RT-qPCR detected the virus in 53.0% (35/66) of the samples.”
✓ located verbatim in the source · abstract, characters 763–915

Title

Digital Polymerase Chain Reaction as a Sensitive Diagnostic Tool: Detection and Quantification of Grapevine Fleck Virus in Grapevine

Min-Jae Kim; Hyo-Jeong Lee; Rae-Dong Jeong Research in Plant Disease 2026. doi:10.5423/rpd.2026.32.1.87

Other claims from this paper (6)

Method / resource The RT-ddPCR assay showed high specificity without cross-reactivity with other grapevine viruses.
Method / resource RT-ddPCR sensitivity was approximately tenfold higher than RT-qPCR.
Method / resource RT-ddPCR had a detection limit of 0.73 copies/μl.
Method / resource RT-ddPCR had high linearity, R2=0.9997.
Method / resource RT-qPCR had high linearity, R2=0.9983.
Method / resource RT-ddPCR detected GFkV in 84.8% of field samples.

Observationobservedoriginal resultmeasured

Claim · model-written summary

GFkV detection was 88.8%.

Taxon: GrapevineVitist0
Interactor: GFkVGrapevine fleck viruse0pathogen
Outcome: virus detection ratese0
Value: 88.8%e0

Verbatim

e0
“Individual virus detection rates were 88.8% for GFkV, 33.5% for GLRaV-1, and 31.6% for GLRaV-3.”
✓ located verbatim in the source · abstract, characters 458–553

t0
“Grapevine”
✓ located verbatim in the source · title, characters 66–75

Title

Occurrence of Three Viruses (GFkV, GLRaV-1, and GLRaV-3) in Major Grapevine-Growing Regions of Korea

Yeseul Choi; Doyeon Cho; Myung-Kyu Song; et al. Research in Plant Disease 2026. doi:10.5423/rpd.2026.32.1.46

Other claims from this paper (19)

Observation Overall grapevine virus infection was 92.7%.
Observation GLRaV-1 detection was 33.5%.
Observation GLRaV-3 detection was 31.6%.
Observation Regional infection incidence in Daejeon was 100%.
Observation Regional infection incidence in Gangwon-do was 100%.
Observation Regional infection incidence in Chungcheongbuk-do was 99.4%.
Observation Regional infection incidence in Chungcheongnam-do was 95.2%.
Observation Regional infection incidence in Jeollabuk-do was 94.2%.
Observation Regional infection incidence in Gyeongsangbukdo was 88.4%.
Observation Regional infection incidence in Gyeongsangnam-do was 87.5%.
Observation Regional infection incidence in Gyeonggi-do was 77.0%.
Observation Infection frequency in Chungrang was 100%.
Observation Infection frequency in Shine Muscat was 95.5%.
Observation Infection frequency in Muscat Bailey-A was 90.5%.
Observation Infection frequency in Campbell Early was 90.1%.
Observation Infection frequency in Kyoho was 84.6%.
Observation Single infections accounted for 44.8% of cases.
Observation double infections accounted for 34.5% of cases.
Observation triple infections accounted for 13.5% of cases.

Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Grapevine fleck virus was among the pests most frequently expected on imported Vitis plants.

Taxon: Vitis spp.Vitist0
Interactor: grapevine fleck virus (GFkV, Maculavirus vitis)Grapevine fleck viruse0pathogen
Outcome: grapevine fleck virus (GFkV, Maculavirus vitis)e0
Setting: Moldovat0

Verbatim

t0
“Vitis spp. plants from Moldova”
✓ located verbatim in the source · title, characters 29–59

e0
“The degree of pest freedom varies among the pests evaluated, with grapevine fleck virus (GFkV, Maculavirus vitis) and ‘Candidatus Phytoplasma solani’ being the pests most frequently expected on the imported plants.”
✓ located verbatim in the source · abstract, characters 1193–1407

Title

Commodity risk assessment of Vitis spp. plants from Moldova

EFSA Panel on Plant Health (PLH); Antonio Vicent Civera; Paula Baptista; et al. EFSA Journal 2026. doi:10.2903/j.efsa.2026.9807

Other claims from this paper (3)

Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Grapevine fleck virus occurred in one nuclear accession.

Taxon: grapevineVitist0
Interactor: grapevine fleck virusGrapevine fleck viruse0pathogen
Outcome: identifiede0
Value: onee0

Verbatim

e0
“while grapevine fleck virus, grapevine Syrah virus 1, grapevine leafroll-associated virus 4, and grapevine Rupestris vein feathering virus were identified in one nuclear accession each.”
✓ located verbatim in the source · abstract, characters 1087–1272

t0
“grapevine”
✓ located verbatim in the source · title, characters 114–123

Title

Application of high-throughput sequencing to detect residual virus and viroid infections in South African nuclear grapevine material

Seamus W. Morgan; Johan T. Burger; Gerhard Pietersen European Journal of Plant Pathology 2026. doi:10.1007/s10658-026-03205-6

Other claims from this paper (15)

Observation Twenty-one of 97 nucleus grapevine accessions had no detectable viruses or viroids.
Observation Five viruses occurred in eight nuclear accessions.
Observation Rupestris stem pitting-associated virus occurred in six nuclear accessions.
Observation Grapevine Syrah virus 1 occurred in one nuclear accession.
Observation Grapevine leafroll-associated virus 4 occurred in one nuclear accession.
Observation Grapevine Rupestris vein feathering virus occurred in one nuclear accession.
Observation HTS identified four viroids and one putative viroid in 76 accessions.
Observation Hop stunt viroid occurred in 63 of 97 samples.
Observation Grapevine yellow speckle viroid 1 occurred in 52 samples.
Observation Grapevine yellow speckle viroid 2 occurred in 17 samples.
Observation Australian grapevine viroid occurred in three samples.
Observation Putative grapevine hammerhead viroid-like RNA occurred in two samples.
Method / resource Specific RT-PCR and Sanger sequencing confirmed all virus and viroid infections.
Recommendation The authors recommend more diverse virus and viroid detection techniques for nuclear certification plants.
Observation Some viruses, especially GRSPaV, and viroids appear recalcitrant to current elimination techniques.

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Ribavirin eliminated GFkV from most tested grapevine plants.

Taxon: GrapevineVitist0
Treatment: ribavirine1
Interactor: GFkVGrapevine fleck viruse0pathogen
Outcome: virus was not detectable by RT-PCRe0
Direction: particularly effectivee0

Not reportedComparator

Verbatim

t0
“Grapevine”
✓ located verbatim in the source · title, characters 79–88

e0
“It proved to be particularly effective (the virus was not detectable by RT-PCR in most of the tested plants) against GFkV, GLRaV-4, GPGV and GRSPaV”
✓ located verbatim in the source · abstract, characters 906–1053

e1
“Among the tested agents, ribavirin had the broadest elimination effect”
✓ located verbatim in the source · abstract, characters 762–832

Title

Effects of Chemotherapy on the Elimination of Various Viruses and Viroids from Grapevine

Mihaly Turcsan; Nikoletta Jaksa-Czotter; Zsuzsanna Nagyne Galbacs; et al. Horticulturae 2025. doi:10.3390/horticulturae12010046

Other claims from this paper (10)

Intervention effect Ribavirin had the broadest virus elimination effect after eight months.
Intervention effect Ribavirin eliminated GLRaV-4 from most tested grapevine plants.
Intervention effect Ribavirin eliminated GPGV from most tested grapevine plants.
Intervention effect Ribavirin eliminated GRSPaV from most tested grapevine plants.
Intervention effect 2-thiouracil caused high phytotoxicity in grapevine.
Intervention effect 2-thiouracil was effective against GLRaV-4.
Intervention effect 2-thiouracil had no effect on other tested viruses.
Intervention effect Zidovudine alone did not eliminate any tested viruses.
Intervention effect None of the three agents eliminated HSVd.
Intervention effect None of the three agents eliminated GYSVd-1.

Observationobservedoriginal resultmeasured

Claim · model-written summary

Overall GFkV infection in grapevine samples was 65.8%.

Taxon: grapevineVitist1
Interactor: Grapevine Fleck Virus (GFkV)Grapevine fleck virust0pathogen
Outcome: infection ratee0
Value: 65.8%e0

Verbatim

e0
“The results revealed a high overall infection rate of 65.8%”
✓ located verbatim in the source · abstract, characters 307–366

t0
“Grapevine Fleck Virus (GFkV) infection”
✓ located verbatim in the source · title, characters 14–52

t1
“grapevine samples from 12 regions”
✓ located verbatim in the source · abstract, characters 153–186

Title

Assessment of Grapevine Fleck Virus (GFkV) infection in Algerian vineyards

Imene Mahdid; Osamah Alisawi; Anfel Djenaoui; et al. Acta Phytopathologica et Entomologica Hungarica 2025. doi:10.1556/038.2025.00238

Other claims from this paper (6)

Observation Central and western Algerian regions had the highest GFkV prevalence.
Observation No GFkV infections were detected in southern regions.
Observation Algerian GFkV isolates had conserved genomic regions.
Observation New Algerian GFkV sequence data were submitted to GenBank.
Observation Italian and Algerian GFkV isolates were phylogenetically related.
Recommendation The authors urged virus monitoring in national certification and plant health programs.

Observationobservedoriginal resultmeasured

Claim · model-written summary

Central and western Algerian regions had the highest GFkV prevalence.

Taxon: grapevineVitist0
Interactor: Grapevine Fleck Virus (GFkV)Grapevine fleck virust1pathogen
Outcome: prevalencee0
Direction: higheste0
Setting: central and western regionse0

Verbatim

e0
“the central and western regions showing the highest prevalence”
✓ located verbatim in the source · abstract, characters 375–437

t0
“grapevine samples from 12 regions”
✓ located verbatim in the source · abstract, characters 153–186

t1
“Grapevine Fleck Virus (GFkV)”
✓ located verbatim in the source · title, characters 14–42

Title

Assessment of Grapevine Fleck Virus (GFkV) infection in Algerian vineyards

Imene Mahdid; Osamah Alisawi; Anfel Djenaoui; et al. Acta Phytopathologica et Entomologica Hungarica 2025. doi:10.1556/038.2025.00238

Other claims from this paper (6)

Observation Overall GFkV infection in grapevine samples was 65.8%.
Observation No GFkV infections were detected in southern regions.
Observation Algerian GFkV isolates had conserved genomic regions.
Observation New Algerian GFkV sequence data were submitted to GenBank.
Observation Italian and Algerian GFkV isolates were phylogenetically related.
Recommendation The authors urged virus monitoring in national certification and plant health programs.

Observationobservedoriginal resultno effectmeasured

Claim · model-written summary

No GFkV infections were detected in southern regions.

Taxon: grapevineVitist0
Interactor: Grapevine Fleck Virus (GFkV)Grapevine fleck virust1pathogen
Outcome: infectionse0
Setting: southern regionse0

Verbatim

e0
“no infections were detected in the southern regions”
✓ located verbatim in the source · abstract, characters 445–496

t0
“grapevine samples from 12 regions”
✓ located verbatim in the source · abstract, characters 153–186

t1
“Grapevine Fleck Virus (GFkV)”
✓ located verbatim in the source · title, characters 14–42

Title

Assessment of Grapevine Fleck Virus (GFkV) infection in Algerian vineyards

Imene Mahdid; Osamah Alisawi; Anfel Djenaoui; et al. Acta Phytopathologica et Entomologica Hungarica 2025. doi:10.1556/038.2025.00238

Other claims from this paper (6)

Observation Overall GFkV infection in grapevine samples was 65.8%.
Observation Central and western Algerian regions had the highest GFkV prevalence.
Observation Algerian GFkV isolates had conserved genomic regions.
Observation New Algerian GFkV sequence data were submitted to GenBank.
Observation Italian and Algerian GFkV isolates were phylogenetically related.
Recommendation The authors urged virus monitoring in national certification and plant health programs.

Observationobservedoriginal resultmeasured

Claim · model-written summary

Algerian GFkV isolates had conserved genomic regions.

Taxon: grapevineVitist0
Interactor: GFkVGrapevine fleck viruse0pathogen
Outcome: conserved genomic regionse0

Verbatim

e0
“Genetic analysis of Algerian GFkV isolates confirmed the presence of conserved genomic regions”
✓ located verbatim in the source · abstract, characters 582–676

t0
“grapevine samples from 12 regions”
✓ located verbatim in the source · abstract, characters 153–186

Title

Assessment of Grapevine Fleck Virus (GFkV) infection in Algerian vineyards

Imene Mahdid; Osamah Alisawi; Anfel Djenaoui; et al. Acta Phytopathologica et Entomologica Hungarica 2025. doi:10.1556/038.2025.00238

Other claims from this paper (6)

Observation Overall GFkV infection in grapevine samples was 65.8%.
Observation Central and western Algerian regions had the highest GFkV prevalence.
Observation No GFkV infections were detected in southern regions.
Observation New Algerian GFkV sequence data were submitted to GenBank.
Observation Italian and Algerian GFkV isolates were phylogenetically related.
Recommendation The authors urged virus monitoring in national certification and plant health programs.

Grapevine fleck virus 158 claims

158 claims