Claim · model-written summary
A 1:2 ammonium to nitrate ratio with 1.5 g/L L-glutamine was optimal for somatic embryogenesis induction.
- Taxon
- P. maximinoi
Pinus maximinoie0 - Treatment
- 1:2 NH 4 + :NO 3 − molar ratio with 1.5 g/L L-glutaminee0
- Outcome
- SE inductione0
Verbatim
e0“The optimal medium for P. maximinoi SE induction contained a 1:2 NH 4 + :NO 3 − molar ratio with 1.5 g/L L-glutamine”
Title
P. S. Nzama; A. A. Myburg; P. N. Hills Plant Cell Tissue and Organ Culture (PCTOC) 2024. doi:10.1007/s11240-024-02748-y
Other claims from this paper (7)
- Intervention effect Ammonium to nitrate ratios of 1:1 and 1:2 produced the most extrusions.
- Intervention effect A 1:4 ammonium to nitrate ratio mostly produced unhealthy, non-embryogenic extrusions.
- Intervention effect Medium supplemented with 1.5 g/L L-glutamine produced rapidly proliferating spiky tissue.
- Intervention effect At a 1:2 ammonium to nitrate ratio, megagametophytes from both plantations formed nodular cultures irrespective of L-glutamine level.
- Association The ammonium to nitrate ratio and L-glutamine acted synergistically to produce the most extrusions.
- Observation The induction competence window extended from the second week of July to the first week of August in both plantations.
- Observation The peak induction period was the fourth week of July 2022.
