Claim · model-written summary
MUR3 and XLT2 regiospecifically galactosylated XXXG, XLXG and XXLG to yield XLLG.
Verbatim
e0“recombinant MUR3 and XLT2 proteins of Arabidopsis, poplar and duckweed were able to regiospecifically galactosylate not only XXXG, but also XLXG and XXLG, respectively, to generate XLLG”
Title
Biochemical insights into the regiospecificity of xyloglucan galactosyltransferases
Ruiqin Zhong; Dennis R Phillips; Zheng-Hua Ye Journal of Experimental Botany 2026. doi:10.1093/jxb/erag117
Other claims from this paper (5)
- Observation The proteins also galactosylated mono- and di-xylosylated xyloglucan oligomers.
- Observation Arabidopsis and poplar MUR3 proteins had an α-helical lid-like domain over their active site clefts.
- Intervention effect Deleting the lid-like domain increased galactosyltransferase activity.
- Observation Docking identified residues that interact with UDP-Gal and XXXG.
- Observation Mutagenesis found substrate-interacting residues critical for catalytic activity.
