Abstract
Few strains of Metschnikowia pulcherrima (Pitt) M.W. Miller are under development for control of postharvest pathogens on fruit. A substrate was developed to optimize the biomass production of M. pulcherrima strain BIO126. Different complex nutrient sources, with or without pH control, were tested. Growth in yeast extract provided at concentrations > or =30 g*L-1 yielded the highest biomass. The addition of two carbon sources, D-mannitol and L-sorbose, at 5 g*L-1 each, significantly improved yeast growth. The greatest amount of yeast growth occurred when pH values of the medium ranged from 5.0 to 7.5. A combination of yeast extract, D-mannitol, and L-sorbose (YEMS), probably with diauxic utilization, showed a synergistic effect, widening the exponential phase (maximum specific growth rate of 0.45 h-1) and increasing the final cell number (1.5 x 109 cells*mL-1) and dry biomass (6.0 g*L-1) in well-controlled batch fermentation. In efficacy trials on 'Golden Delicious' apples, M. pulcherrima grown in YEMS effectively reduced incidence and severity of Botrytis cinerea (51.1% and 70.8%, respectively) and Penicillium expansum (41.7% and 14.0%, respectively). Also on 'Gala' apples, the best reduction of grey and blue mould incidence was obtained with cells grown in YEMS (58.1% and 50.5%, respectively).
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Claims 12
Claim · model-written summary
Yeast extract at concentrations of 30 g/L or more gave the highest M. pulcherrima biomass.
- Taxon
- apples
Malus domesticae1 - Treatment
- yeast extracte2
- Outcome
- biomasse2
- Value
- > or =30 g*L-1e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“'Golden Delicious' apples”
e2“Growth in yeast extract provided at concentrations > or =30 g*L-1 yielded the highest biomass”
Claim · model-written summary
Adding D-mannitol and L-sorbose at 5 g/L each significantly improved M. pulcherrima yeast growth.
- Taxon
- applesMalus domesticae1
- Treatment
- D-mannitol and L-sorbosee2
- Outcome
- yeast growthe2
- Value
- 5 g*L-1 eache2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“'Golden Delicious' apples”
e2“The addition of two carbon sources, D-mannitol and L-sorbose, at 5 g*L-1 each, significantly improved yeast growth”
Claim · model-written summary
The greatest amount of M. pulcherrima growth occurred at a medium pH of 5.0 to 7.5.
- Taxon
- applesMalus domesticae1
- Treatment
- pH values of the mediume2
- Outcome
- amount of yeast growthe2
- Value
- 5.0 to 7.5e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“'Golden Delicious' apples”
e2“The greatest amount of yeast growth occurred when pH values of the medium ranged from 5.0 to 7.5”
Claim · model-written summary
YEMS medium gave M. pulcherrima a maximum specific growth rate of 0.45 per hour with a synergistic effect widening the exponential phase.
- Taxon
- applesMalus domesticae1
- Treatment
- YEMSe2
- Outcome
- maximum specific growth ratee3
- Value
- 0.45 h-1e3
- Qualifier
- showed a synergistic effect, widening the exponential phasee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“'Golden Delicious' apples”
e2“A combination of yeast extract, D-mannitol, and L-sorbose (YEMS)”
e3“showed a synergistic effect, widening the exponential phase (maximum specific growth rate of 0.45 h-1)”
Claim · model-written summary
YEMS medium gave a final M. pulcherrima cell number of 1.5 x 10^9 cells per mL.
- Taxon
- applesMalus domesticae1
- Treatment
- YEMSe2
- Outcome
- final cell numbere3
- Value
- 1.5 x 109 cells*mL-1e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“'Golden Delicious' apples”
e2“A combination of yeast extract, D-mannitol, and L-sorbose (YEMS)”
e3“final cell number (1.5 x 109 cells*mL-1)”
Claim · model-written summary
YEMS medium gave a M. pulcherrima dry biomass of 6.0 g/L.
- Taxon
- applesMalus domesticae1
- Treatment
- YEMSe2
- Outcome
- dry biomasse3
- Value
- 6.0 g*L-1e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“'Golden Delicious' apples”
e2“A combination of yeast extract, D-mannitol, and L-sorbose (YEMS)”
e3“dry biomass (6.0 g*L-1)”
Claim · model-written summary
M. pulcherrima grown in YEMS reduced the incidence of Botrytis cinerea on Golden Delicious apples by 51.1%.
- Taxon
- applesMalus domesticae1
- Treatment
- M. pulcherrima grown in YEMSe2
- Interactor
- Botrytis cinerea
Botrytis cinereae2pathogen - Second interactor
- M. pulcherrimaMetschnikowia pulcherrimae2biocontrol_agent
- Outcome
- incidencee2
- Direction
- reducede2
- Value
- 51.1%e2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“'Golden Delicious' apples”
e2“M. pulcherrima grown in YEMS effectively reduced incidence and severity of Botrytis cinerea (51.1% and 70.8%, respectively) and Penicillium expansum (41.7% and 14.0%, respectively)”
Claim · model-written summary
M. pulcherrima grown in YEMS reduced the severity of Botrytis cinerea on Golden Delicious apples by 70.8%.
- Taxon
- applesMalus domesticae1
- Treatment
- M. pulcherrima grown in YEMSe2
- Interactor
- Botrytis cinereaBotrytis cinereae2pathogen
- Second interactor
- M. pulcherrimaMetschnikowia pulcherrimae2biocontrol_agent
- Outcome
- severitye2
- Direction
- reducede2
- Value
- 70.8%e2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“'Golden Delicious' apples”
e2“M. pulcherrima grown in YEMS effectively reduced incidence and severity of Botrytis cinerea (51.1% and 70.8%, respectively) and Penicillium expansum (41.7% and 14.0%, respectively)”
Claim · model-written summary
M. pulcherrima grown in YEMS reduced the incidence of Penicillium expansum on Golden Delicious apples by 41.7%.
- Taxon
- applesMalus domesticae1
- Treatment
- M. pulcherrima grown in YEMSe2
- Interactor
- Penicillium expansum
Penicillium expansume2pathogen - Second interactor
- M. pulcherrimaMetschnikowia pulcherrimae2biocontrol_agent
- Outcome
- incidencee2
- Direction
- reducede2
- Value
- 41.7%e2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“'Golden Delicious' apples”
e2“M. pulcherrima grown in YEMS effectively reduced incidence and severity of Botrytis cinerea (51.1% and 70.8%, respectively) and Penicillium expansum (41.7% and 14.0%, respectively)”
Claim · model-written summary
M. pulcherrima grown in YEMS reduced the severity of Penicillium expansum on Golden Delicious apples by 14.0%.
- Taxon
- applesMalus domesticae1
- Treatment
- M. pulcherrima grown in YEMSe2
- Interactor
- Penicillium expansumPenicillium expansume2pathogen
- Second interactor
- M. pulcherrimaMetschnikowia pulcherrimae2biocontrol_agent
- Outcome
- severitye2
- Direction
- reducede2
- Value
- 14.0%e2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“'Golden Delicious' apples”
e2“M. pulcherrima grown in YEMS effectively reduced incidence and severity of Botrytis cinerea (51.1% and 70.8%, respectively) and Penicillium expansum (41.7% and 14.0%, respectively)”
Claim · model-written summary
On Gala apples, cells grown in YEMS gave the best reduction of grey mould incidence, by 58.1%.
- Taxon
- applesMalus domesticae1
- Treatment
- cells grown in YEMSe1
- Outcome
- grey and blue mould incidencee1
- Direction
- reductione1
- Value
- 58.1%e1
- Qualifier
- greye1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“on 'Gala' apples, the best reduction of grey and blue mould incidence was obtained with cells grown in YEMS (58.1% and 50.5%, respectively)”
Claim · model-written summary
On Gala apples, cells grown in YEMS gave the best reduction of blue mould incidence, by 50.5%.
- Taxon
- applesMalus domesticae1
- Treatment
- cells grown in YEMSe1
- Outcome
- grey and blue mould incidencee1
- Direction
- reductione1
- Value
- 50.5%e1
- Qualifier
- bluee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“on 'Gala' apples, the best reduction of grey and blue mould incidence was obtained with cells grown in YEMS (58.1% and 50.5%, respectively)”
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See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.