Abstract
In Arabidopsis thaliana, acyl-CoA-binding proteins (ACBPs) are encoded by a family of six genes (ACBP1 to ACBP6), and are essential for diverse cellular activities. Recent investigations suggest that the membrane-anchored ACBPs are involved in oxygen sensing by sequestration of group VII ethylene-responsive factors under normoxia. Here, we demonstrate the involvement of Arabidopsis ACBP3 in hypoxic tolerance. ACBP3 transcription was remarkably induced following submergence under both dark (DS) and light (LS) conditions. ACBP3-overexpressors (ACBP3-OEs) showed hypersensitivity to DS, LS and ethanolic stresses, with reduced transcription of hypoxia-responsive genes as well as accumulation of hydrogen peroxide in the rosettes. In contrast, suppression of ACBP3 in ACBP3-KOs enhanced plant tolerance to DS, LS and ethanol treatments. By analyses of double combinations of OE-1 with npr1-5, coi1-2, ein3-1 as well as ctr1-1 mutants, we observed that the attenuated hypoxic tolerance in ACBP3-OEs was dependent on NPR1- and CTR1-mediated signaling pathways. Lipid profiling revealed that both the total amounts and very-long-chain species of phosphatidylserine (C42:2- and C42:3-PS) and glucosylinositolphosphorylceramides (C22:0-, C22:1-, C24:0-, C24:1-, and C26:1-GIPC) were significantly lower in ACBP3-OEs but increased in ACBP3-KOs upon LS exposure. By microscale thermophoresis analysis, the recombinant ACBP3 protein bound VLC acyl-CoA esters with high affinities in vitro. Further, a knockout mutant of MYB30, a master regulator of very-long-chain fatty acid (VLCFA) biosynthesis, exhibited enhanced sensitivities to LS and ethanolic stresses, phenotypes that were ameliorated by ACBP3-RNAi. Taken together, these findings suggest that Arabidopsis ACBP3 participates in plant response to hypoxia by modulating VLCFA metabolism.
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Claims 14
Claim · model-written summary
Submergence induced ACBP3 transcription under dark and light.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Treatment
- submergencee0
- Gene
- ACBP3e0
- Outcome
- ACBP3 transcriptione0
- Direction
- inducede0
- Qualifier
- both dark (DS) and light (LS) conditionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ACBP3 transcription was remarkably induced following submergence under both dark (DS) and light (LS) conditions”
t0“Arabidopsis thaliana”
Claim · model-written summary
ACBP3 overexpressors were hypersensitive to dark submergence, light submergence and ethanol.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- ACBP3-overexpressors (ACBP3-OEs)e0
- Treatment
- DS, LS and ethanolic stressese0
- Gene
- ACBP3e0
- Outcome
- hypersensitivitye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ACBP3-overexpressors (ACBP3-OEs) showed hypersensitivity to DS, LS and ethanolic stresses”
t0“Arabidopsis thaliana”
Claim · model-written summary
ACBP3 overexpressors had reduced hypoxia-responsive gene transcription.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Outcome
- transcription of hypoxia-responsive genese0
- Direction
- reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“with reduced transcription of hypoxia-responsive genes”
t0“Arabidopsis thaliana”
Claim · model-written summary
ACBP3 overexpressors accumulated hydrogen peroxide in rosettes.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Organ / stage
- rosettese0
- Outcome
- accumulation of hydrogen peroxidee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“accumulation of hydrogen peroxide in the rosettes”
t0“Arabidopsis thaliana”
Claim · model-written summary
ACBP3 suppression enhanced tolerance to dark and light submergence and ethanol.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- ACBP3-KOse0
- Treatment
- suppression of ACBP3e0
- Gene
- ACBP3e0
- Outcome
- plant tolerance to DS, LS and ethanol treatmentse0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suppression of ACBP3 in ACBP3-KOs enhanced plant tolerance to DS, LS and ethanol treatments”
t0“Arabidopsis thaliana”
Claim · model-written summary
Reduced hypoxic tolerance in overexpressors depended on NPR1 and CTR1 signaling.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- ACBP3-OEse0
- Gene
- NPR1- and CTR1-mediated signaling pathwayse0
- Outcome
- attenuated hypoxic tolerancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“attenuated hypoxic tolerance in ACBP3-OEs was dependent on NPR1- and CTR1-mediated signaling pathways”
t0“Arabidopsis thaliana”
Claim · model-written summary
ACBP3 overexpression lowered phosphatidylserine amounts under light submergence.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- ACBP3-OEse0
- Treatment
- LS exposuree0
- Outcome
- total amounts and very-long-chain species of phosphatidylserinee0
- Direction
- lowere0
- Qualifier
- C42:2- and C42:3-PSe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both the total amounts and very-long-chain species of phosphatidylserine (C42:2- and C42:3-PS) and glucosylinositolphosphorylceramides (C22:0-, C22:1-, C24:0-, C24:1-, and C26:1-GIPC) were significantly lower in ACBP3-OEs but increased in ACBP3-KOs upon LS exposure”
t0“Arabidopsis thaliana”
Claim · model-written summary
ACBP3 overexpression lowered glucosylinositolphosphorylceramides under light submergence.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- ACBP3-OEse0
- Treatment
- LS exposuree0
- Outcome
- glucosylinositolphosphorylceramidese0
- Direction
- lowere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“glucosylinositolphosphorylceramides (C22:0-, C22:1-, C24:0-, C24:1-, and C26:1-GIPC) were significantly lower in ACBP3-OEs but increased in ACBP3-KOs upon LS exposure”
t0“Arabidopsis thaliana”
Claim · model-written summary
ACBP3 knockout increased phosphatidylserine under light submergence.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- ACBP3-KOse0
- Treatment
- LS exposuree0
- Outcome
- phosphatidylserinee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“phosphatidylserine (C42:2- and C42:3-PS) and glucosylinositolphosphorylceramides (C22:0-, C22:1-, C24:0-, C24:1-, and C26:1-GIPC) were significantly lower in ACBP3-OEs but increased in ACBP3-KOs upon LS exposure”
t0“Arabidopsis thaliana”
Claim · model-written summary
ACBP3 knockout increased glucosylinositolphosphorylceramides under light submergence.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- ACBP3-KOse0
- Treatment
- LS exposuree0
- Outcome
- glucosylinositolphosphorylceramidese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“glucosylinositolphosphorylceramides (C22:0-, C22:1-, C24:0-, C24:1-, and C26:1-GIPC) were significantly lower in ACBP3-OEs but increased in ACBP3-KOs upon LS exposure”
t0“Arabidopsis thaliana”
Claim · model-written summary
ACBP3 bound very-long-chain acyl-CoA esters with high affinity in vitro.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- ACBP3 proteine0
- Outcome
- bound VLC acyl-CoA esters with high affinitiese0
- Setting
- in vitroe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the recombinant ACBP3 protein bound VLC acyl-CoA esters with high affinities in vitro”
t0“Arabidopsis thaliana”
Claim · model-written summary
MYB30 knockout increased sensitivity to light submergence and ethanol.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- LS and ethanolic stressese0
- Gene
- MYB30e0
- Outcome
- sensitivitiese0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a knockout mutant of MYB30, a master regulator of very-long-chain fatty acid (VLCFA) biosynthesis, exhibited enhanced sensitivities to LS and ethanolic stresses”
t0“Arabidopsis thaliana”
Claim · model-written summary
ACBP3 RNAi ameliorated MYB30 knockout sensitivity.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- ACBP3-RNAie0
- Gene
- ACBP3-RNAie0
- Outcome
- phenotypese0
- Direction
- amelioratede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“phenotypes that were ameliorated by ACBP3-RNAi”
t0“Arabidopsis thaliana”
Claim · model-written summary
ACBP3 may modulate VLCFA metabolism to affect hypoxia response.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- ACBP3e0
- Outcome
- plant response to hypoxiae0
- Qualifier
- modulating VLCFA metabolisme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis ACBP3 participates in plant response to hypoxia by modulating VLCFA metabolism”
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