Abstract
The wound-induced receptor-like protein kinase (WRK) gene, isolated as one of the genes whose transcripts accumulated during the early period of N gene-dependent synchronized cell death in tobacco mosaic virus-infected tobacco plants, encodes a leucine-rich repeat receptor-like protein kinase, and its transcript was transiently increased 15 min after wounding. In the present study, analysis of a green fluorescent protein fusion protein indicated that WRK is localized in the plasma membrane. In transgenic tobacco plant lines with elevated or suppressed levels of WRK transcript, the wound-induced accumulation of both basic PR-1 and PR-6 transcripts was slightly enhanced or significantly suppressed respectively. The decrease in wound-induced basic PR gene expression in WRK suppressed lines was restored by jasmonic acid (JA) treatment. Furthermore, the levels of wound-induced enzymatic activation of both salicylic acid-induced protein kinase (SIPK) and wound-induced protein kinase (WIPK) and wound-induced accumulation of JA were reduced in the WRK suppressed lines in comparison with a control line. These results suggest that WRK functions upstream of SIPK and WIPK and regulates wound signal transduction in tobacco plants.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
WRK localizes to the plasma membrane.
- Taxon
- tobacco
Nicotiana tabacumt0 - Gene
- WRKe0
- Outcome
- localized in the plasma membranee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the present study, analysis of a green fluorescent protein fusion protein indicated that WRK is localized in the plasma membrane.”
t0“tobacco plants”
Claim · model-written summary
Elevated WRK transcript slightly enhanced wound induced basic PR-1 and PR-6 transcripts.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- elevatede0
- Gene
- WRKe0
- Outcome
- wound-induced accumulation of both basic PR-1 and PR-6 transcriptse0
- Direction
- slightly enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In transgenic tobacco plant lines with elevated or suppressed levels of WRK transcript, the wound-induced accumulation of both basic PR-1 and PR-6 transcripts was slightly enhanced or significantly suppressed respectively.”
Claim · model-written summary
Suppressed WRK transcript significantly suppressed wound induced basic PR-1 and PR-6 transcripts.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- suppressed levels of WRK transcripte0
- Gene
- WRKe0
- Outcome
- wound-induced accumulation of both basic PR-1 and PR-6 transcriptse0
- Direction
- significantly suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In transgenic tobacco plant lines with elevated or suppressed levels of WRK transcript, the wound-induced accumulation of both basic PR-1 and PR-6 transcripts was slightly enhanced or significantly suppressed respectively.”
Claim · model-written summary
Jasmonic acid restored wound induced basic PR expression in WRK suppressed lines.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- jasmonic acid (JA) treatmente0
- Gene
- WRKe0
- Outcome
- wound-induced basic PR gene expressione0
- Direction
- restorede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The decrease in wound-induced basic PR gene expression in WRK suppressed lines was restored by jasmonic acid (JA) treatment.”
t0“tobacco plants”
Claim · model-written summary
WRK suppression reduced wound induced SIPK and WIPK activation versus a control line.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- WRK suppressed linese0
- Gene
- SIPKe0
- Outcome
- wound-induced enzymatic activation of both salicylic acid-induced protein kinase (SIPK) and wound-induced protein kinase (WIPK)e0
- Direction
- reducede0
- Comparator
- a control linee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Furthermore, the levels of wound-induced enzymatic activation of both salicylic acid-induced protein kinase (SIPK) and wound-induced protein kinase (WIPK) and wound-induced accumulation of JA were reduced in the WRK suppressed lines in comparison with a control line.”
t0“tobacco plants”
Claim · model-written summary
WRK suppression reduced wound induced JA accumulation versus a control line.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- WRK suppressed linese0
- Gene
- WRKe0
- Outcome
- wound-induced accumulation of JAe0
- Direction
- reducede0
- Comparator
- a control linee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Furthermore, the levels of wound-induced enzymatic activation of both salicylic acid-induced protein kinase (SIPK) and wound-induced protein kinase (WIPK) and wound-induced accumulation of JA were reduced in the WRK suppressed lines in comparison with a control line.”
t0“tobacco plants”
Claim · model-written summary
The authors infer that WRK acts upstream of SIPK and WIPK in tobacco wound signaling.
- Taxon
- tobaccoNicotiana tabacume0
- Gene
- WRKe0
- Outcome
- wound signal transductione0
- Qualifier
- upstream of SIPK and WIPKe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These results suggest that WRK functions upstream of SIPK and WIPK and regulates wound signal transduction in tobacco plants.”
The same questions in other papers
jasmonic acid · molecular: expression · Nicotiana tabacum (tobacco)Positive and negative results
5 papers in the same direction as this paper · 4 in the opposite direction
- negative
- no effect
- no effect
6 papers more in this group
See all 10 papers →mechanical · molecular: expression · Nicotiana tabacum (tobacco)Positive and negative results
3 papers in the same direction as this paper · 17 in the opposite direction
- positive
- positive
- positive
17 papers more in this group
See all 21 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.