Abstract
A tobacco peroxidase gene tpoxN1 was reported to be expressed within 1 h after wounding in leaves [Hiraga et al. (2000a) Plant Cell Physiol. 41: 165]. We describe here further results on the wound-induced tpoxN1 expression. The quick tpoxN1 induction occurred preferentially in stems and petioles, but was negligible in leaf blades even 8 h after wounding. Induced GUS activity was also detected rapidly after wounding in the stem of transgenic tobacco plants carrying the tpoxN1 promoter::GUS fusion gene, localized mainly in the vascular systems where it was maintained this level for 14 d or more. Strong GUS activity was also found in the petiole and veinlet as well as the epidermal tissue in the stem. Treatment of known inducers for wound-responsive genes such as jasmonate, 1-aminocyclopropane-1-carboxylate, spermine, phytohormones and other stress treatments did not enhance wound-induced tpoxN1 gene expression in stems at all, but rather repressed it in some cases. Studies using metabolic inhibitors suggested that phosphorylation and dephosphorylation of proteins together with de novo protein synthesis are likely to be involved in the wound-induced tpoxN1 expression as well as some other wound-responsive genes. Thus, tpoxN1 is a unique wound-inducible and possible wound-healing gene which is rapidly expressed being maintained for a long time in veins via an unknown wound-signaling pathway(s).
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 5
Claim · model-written summary
Wounding quickly induced tpoxN1 preferentially in stems and petioles, with negligible induction in leaf blades after eight hours.
- Taxon
- Tobacco
Nicotiana tabacumt0 - Organ / stage
- stems and petiolese0
- Treatment
- woundinge0
- Gene
- tpoxN1e0
- Outcome
- tpoxN1 inductione0
- Direction
- preferentiallye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The quick tpoxN1 induction occurred preferentially in stems and petioles, but was negligible in leaf blades even 8 h after wounding.”
t0“Tobacco”
Claim · model-written summary
Wounding rapidly induced GUS activity in transgenic tobacco stems, mainly in vascular systems, with persistence for at least 14 days.
- Taxon
- tobaccoNicotiana tabacume0
- Organ / stage
- vascular systemse0
- Treatment
- woundinge0
- Gene
- tpoxN1 promoter::GUS fusion genee0
- Outcome
- GUS activitye0
- Direction
- Inducede0
- Qualifier
- 14 d or moree0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Induced GUS activity was also detected rapidly after wounding in the stem of transgenic tobacco plants carrying the tpoxN1 promoter::GUS fusion gene, localized mainly in the vascular systems where it was maintained this level for 14 d or more.”
Claim · model-written summary
Strong GUS activity occurred in tobacco petiole, veinlet and stem epidermis.
- Taxon
- TobaccoNicotiana tabacumt0
- Organ / stage
- petiole and veinlet as well as the epidermal tissue in the steme0
- Outcome
- GUS activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Strong GUS activity was also found in the petiole and veinlet as well as the epidermal tissue in the stem.”
t0“Tobacco”
Claim · model-written summary
Known wound-response inducers did not enhance wound-induced tpoxN1 expression in stems and sometimes repressed it.
- Taxon
- TobaccoNicotiana tabacumt0
- Organ / stage
- stemse0
- Treatment
- jasmonate, 1-aminocyclopropane-1-carboxylate, spermine, phytohormones and other stress treatmentse0
- Gene
- tpoxN1e0
- Outcome
- wound-induced tpoxN1 gene expressione0
- Direction
- did not enhancee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment of known inducers for wound-responsive genes such as jasmonate, 1-aminocyclopropane-1-carboxylate, spermine, phytohormones and other stress treatments did not enhance wound-induced tpoxN1 gene expression in stems at all, but rather repressed it in some cases.”
t0“Tobacco”
Claim · model-written summary
Inhibitor studies suggested protein phosphorylation, dephosphorylation and new protein synthesis contribute to wound-induced tpoxN1 expression.
- Taxon
- TobaccoNicotiana tabacumt0
- Gene
- tpoxN1e0
- Outcome
- wound-induced tpoxN1 expressione0
- Qualifier
- phosphorylation and dephosphorylation of proteins together with de novo protein synthesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Studies using metabolic inhibitors suggested that phosphorylation and dephosphorylation of proteins together with de novo protein synthesis are likely to be involved in the wound-induced tpoxN1 expression”
t0“Tobacco”
The same questions in other papers
spermine · molecular: expression · Nicotiana tabacum (tobacco)Positive and negative results
5 in the opposite direction
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →jasmonic acid · molecular: expression · Nicotiana tabacum (tobacco)Positive and negative results
3 papers in the same direction as this paper · 6 in the opposite direction
- positive
- positive
- positive
6 papers more in this group
See all 10 papers →class · molecular: expression · Nicotiana tabacum (tobacco)Positive and negative results
12 papers in the same direction as this paper · 14 in the opposite direction
- positive
- positive
- positive
23 papers more in this group
See all 27 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.