Abstract
Abstract Effects were examined of barley powdery mildew ( Erysiphe graminis f. sp. hordei ) on the major leaf protein, ribulose‐l,5‐bisphosphate carboxylase (RuBPCase), and other enzymes of CO 2 assimilation, phosphoenolpyruvate carboxylase (PEPcase) and malic enzymes, and enzymes associated with RuBPCase in the reductive pentose phosphate pathway. Activity of RuBPCase per unit fresh weight of leaf was inhibited by infection from the first sample, 3 days after inoculation, to the last sample, 24 days after inoculation, when healthy control leaves were visibly senescing. The inhibition occurred because the amount of RuBPCase protein (measured specifically by an immunological technique) was reduced from 6 days after inoculation and because activity per unit protein declined from 3 days until 21 days after inoculation, at which time there was little protein remaining. Activity of PEPcase per unit fresh weight of leaf was initially stimulated by infection but, thereafter, it was inhibited. Inhibition, like that also affecting malic enzymes (NAD) and (NADP), 3‐phosphoglycerate kinase, and glyceraldehyde‐3‐phosphate dehydrogenases (NAD) and (NADP), was associated with a decline in amounts of total soluble minus RuBP Case protein per unit fresh weight of leaf. Reduced amounts of leaf protein may be associated with reduced nitrate uptake by roots and fungal demand for nitrogen in mildewed plants.
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Claims 7
Claim · model-written summary
Powdery mildew inhibited barley leaf RuBPCase activity from day 3 through day 24.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaret0 - Organ / stage
- leafe0
- Treatment
- infectione0
- Gene
- RuBPCasee0
- Outcome
- Activity of RuBPCase per unit fresh weight of leafe0
- Direction
- inhibitede0
- Qualifier
- 3 days after inoculation, to the last sample, 24 days after inoculatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Activity of RuBPCase per unit fresh weight of leaf was inhibited by infection from the first sample, 3 days after inoculation, to the last sample, 24 days after inoculation”
t0“Ribulose Bisphosphate Carboxylase Protein and Enzymes of CO2 Assimilation in Barley”
Claim · model-written summary
Infection reduced RuBPCase protein amount from day 6.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- inoculatione0
- Gene
- RuBPCasee0
- Outcome
- amount of RuBPCase proteine0
- Direction
- reducede0
- Qualifier
- from 6 days after inoculatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the amount of RuBPCase protein (measured specifically by an immunological technique) was reduced from 6 days after inoculation”
t0“Ribulose Bisphosphate Carboxylase Protein and Enzymes of CO2 Assimilation in Barley”
Claim · model-written summary
RuBPCase activity per unit protein declined from day 3 to 21.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- activity per unit proteine0
- Direction
- declinede0
- Qualifier
- from 3 days until 21 days after inoculatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“activity per unit protein declined from 3 days until 21 days after inoculation”
t0“Ribulose Bisphosphate Carboxylase Protein and Enzymes of CO2 Assimilation in Barley”
Claim · model-written summary
Infection initially stimulated barley PEPcase activity.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- infectione0
- Gene
- PEPcasee0
- Outcome
- Activity of PEPcase per unit fresh weight of leafe0
- Direction
- initially stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Activity of PEPcase per unit fresh weight of leaf was initially stimulated by infection”
t0“Ribulose Bisphosphate Carboxylase Protein and Enzymes of CO2 Assimilation in Barley”
Claim · model-written summary
Infection subsequently inhibited barley PEPcase activity.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- infectione0
- Gene
- PEPcasee0
- Outcome
- Activity of PEPcase per unit fresh weight of leafe0
- Direction
- inhibitede0
- Qualifier
- thereaftere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Activity of PEPcase per unit fresh weight of leaf was initially stimulated by infection but, thereafter, it was inhibited”
t0“Ribulose Bisphosphate Carboxylase Protein and Enzymes of CO2 Assimilation in Barley”
Claim · model-written summary
Malic enzymes, 3-phosphoglycerate kinase and glyceraldehyde-3-phosphate dehydrogenases were inhibited.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- malic enzymes (NAD) and (NADP), 3‐phosphoglycerate kinase, and glyceraldehyde‐3‐phosphate dehydrogenases (NAD) and (NADP)e0
- Direction
- Inhibitione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inhibition, like that also affecting malic enzymes (NAD) and (NADP), 3‐phosphoglycerate kinase, and glyceraldehyde‐3‐phosphate dehydrogenases (NAD) and (NADP), was associated with a decline in amounts of total soluble minus RuBP Case protein per unit fresh weight of leaf”
t0“Ribulose Bisphosphate Carboxylase Protein and Enzymes of CO2 Assimilation in Barley”
Claim · model-written summary
Enzyme inhibition was associated with lower soluble non-RuBPCase protein.
- Taxon
- barleyHordeum vulgaret0
- Gene
- RuBP Casee0
- Outcome
- amounts of total soluble minus RuBP Case protein per unit fresh weight of leafe0
- Direction
- declinee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inhibition, like that also affecting malic enzymes (NAD) and (NADP), 3‐phosphoglycerate kinase, and glyceraldehyde‐3‐phosphate dehydrogenases (NAD) and (NADP), was associated with a decline in amounts of total soluble minus RuBP Case protein per unit fresh weight of leaf”
t0“Ribulose Bisphosphate Carboxylase Protein and Enzymes of CO2 Assimilation in Barley”
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See all 16 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.