Abstract
In this work, we investigated the effects of pH and nitrogen forms on iron homeostasis and the expression profiles of genes involved in iron uptake and metabolism using tomato cultivar T3238 and its iron-inefficient mutant T3238fer. We showed that high external pH led to increased expression of four iron uptake genes (LeIRT1, LeIRT2, LeFRO1, LeNRAMP1) regardless of the nitrogen sources. Interestingly, the transcript level of FER was decreased at high pH and increased at low pH. In iron-inefficient mutant T3238fer, the expression of LeFRO1, LeIRT1 and LeNRAMP1 was much less than wild type under the culture conditions with high pH and on the non-buffered agar medium with NO(3) (-) as the sole N source, demonstrating that FER protein is required for the increased expression of LeFRO1, LeIRT1 and LeNRAMP1 under culture conditions with high pH. Considering the paradoxical expression patterns of FER to LeFRO1, LeIRT1 and LeNRAMP1 in T3238, we speculate that FER is essential, but is not the limited factor for the transcriptional regulation of the three iron uptake genes. In conclusion, the alteration of rhizosphere pH by assimilating NO(3) (-) or NH(4) (+) influenced Fe availability and consequently affected iron homeostasis in tomato. The enhanced expression of LeFRO1, LeIRT1 and LeNRAMP1 under the culture condition with high pH or on agar media with NO(3) (-) as the sole N source might be a consequence of reduced iron availability in the solution or agar medium at high pH.
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Claims 8
Claim · model-written summary
High external pH increased expression of four iron uptake genes regardless of nitrogen source.
- Taxon
- tomato
Solanum lycopersicumt0 - Treatment
- high external pHe0
- Outcome
- expression of four iron uptake genes (LeIRT1, LeIRT2, LeFRO1, LeNRAMP1)e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato”
e0“high external pH led to increased expression of four iron uptake genes (LeIRT1, LeIRT2, LeFRO1, LeNRAMP1) regardless of the nitrogen sources”
Claim · model-written summary
FER transcript level decreased at high pH.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- high pHe0
- Gene
- FERe0
- Outcome
- transcript level of FERe0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato”
e0“the transcript level of FER was decreased at high pH and increased at low pH”
Claim · model-written summary
FER transcript level increased at low pH.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- low pHe0
- Gene
- FERe0
- Outcome
- transcript level of FERe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato”
e0“the transcript level of FER was decreased at high pH and increased at low pH”
Claim · model-written summary
The mutant had much less LeFRO1, LeIRT1 and LeNRAMP1 expression than wild type under high pH and nitrate-only agar conditions.
- Taxon
- tomatoSolanum lycopersicumt0
- Material
- T3238fere0
- Gene
- LeFRO1, LeIRT1 and LeNRAMP1e0
- Outcome
- expression of LeFRO1, LeIRT1 and LeNRAMP1e0
- Direction
- much lesse0
- Qualifier
- high pH and on the non-buffered agar medium with NO(3) (-) as the sole N sourcee0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato”
e0“In iron-inefficient mutant T3238fer, the expression of LeFRO1, LeIRT1 and LeNRAMP1 was much less than wild type under the culture conditions with high pH and on the non-buffered agar medium with NO(3) (-) as the sole N source”
Claim · model-written summary
FER protein was interpreted as required for increased expression of three iron uptake genes at high pH.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- FER proteine0
- Outcome
- increased expression of LeFRO1, LeIRT1 and LeNRAMP1e0
- Qualifier
- under culture conditions with high pHe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato”
e0“FER protein is required for the increased expression of LeFRO1, LeIRT1 and LeNRAMP1 under culture conditions with high pH”
Claim · model-written summary
FER was proposed to be essential but not limiting for transcriptional regulation of the three uptake genes.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- FERe0
- Outcome
- transcriptional regulation of the three iron uptake genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato”
e0“FER is essential, but is not the limited factor for the transcriptional regulation of the three iron uptake genes”
Claim · model-written summary
Nitrogen-driven alteration of rhizosphere pH influenced iron availability and tomato iron homeostasis.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- assimilating NO(3) (-) or NH(4) (+)e0
- Outcome
- iron homeostasis in tomatoe0
- Qualifier
- alteration of rhizosphere pHe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the alteration of rhizosphere pH by assimilating NO(3) (-) or NH(4) (+) influenced Fe availability and consequently affected iron homeostasis in tomato”
Claim · model-written summary
Reduced iron availability at high pH was proposed to explain enhanced expression of three uptake genes.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- LeFRO1, LeIRT1 and LeNRAMP1e0
- Outcome
- enhanced expression of LeFRO1, LeIRT1 and LeNRAMP1e0
- Qualifier
- reduced iron availability in the solution or agar medium at high pHe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato”
e0“The enhanced expression of LeFRO1, LeIRT1 and LeNRAMP1 under the culture condition with high pH or on agar media with NO(3) (-) as the sole N source might be a consequence of reduced iron availability in the solution or agar medium at high pH”
The same questions in other papers
alkalinity · molecular: expression · Solanum lycopersicum (tomato)Positive and negative results
3 in the opposite direction
- positive
- positive
- positive
ph · molecular: expression · Solanum lycopersicum (tomato)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.