Abstract
The Arabidopsis thaliana metal tolerance protein 1 (MTP1) of the cation diffusion facilitator family of membrane transport proteins can mediate the detoxification of Zn in Arabidopsis and yeast. Xenopus laevis oocytes expressing AtMTP1 accumulate more Zn than oocytes expressing the AtMTP1(D94A) mutant or water-injected oocytes. An AtMTP1-GFP fusion protein localizes to the vacuolar membrane in root and leaf cells. The analysis of Arabidopsis transformed with a promoter-GUS construct suggests that AtMTP1 is not produced throughout the plant, but primarily in the subpopulation of dividing, differentiating and expanding cells. RNA interference-mediated silencing of AtMTP1 causes Zn hypersensitivity and a reduction in Zn concentrations in vegetative plant tissues.
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Claims 6
Claim · model-written summary
The Arabidopsis thaliana metal tolerance protein 1 (MTP1) can mediate Zn detoxification in Arabidopsis and yeast.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Gene
- metal tolerance protein 1 (MTP1)e1
- Outcome
- detoxification of Zne1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana”
e1“The Arabidopsis thaliana metal tolerance protein 1 (MTP1) of the cation diffusion facilitator family of membrane transport proteins can mediate the detoxification of Zn in Arabidopsis and yeast”
Claim · model-written summary
Xenopus laevis oocytes expressing AtMTP1 accumulated more Zn than oocytes expressing the AtMTP1(D94A) mutant or water-injected oocytes.
- Taxon
- Xenopus laevis
Xenopus laevise1 - Treatment
- expressing AtMTP1e1
- Gene
- AtMTP1e1
- Outcome
- Zne1
- Direction
- moree1
- Comparator
- oocytes expressing the AtMTP1(D94A) mutant or water-injected oocytese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Xenopus laevis oocytes expressing AtMTP1 accumulate more Zn than oocytes expressing the AtMTP1(D94A) mutant or water-injected oocytes”
Claim · model-written summary
An AtMTP1-GFP fusion protein localizes to the vacuolar membrane in root and leaf cells.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Organ / stage
- root and leaf cellse1
- Gene
- AtMTP1-GFPe1
- Outcome
- localizes to the vacuolar membranee1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana”
e1“An AtMTP1-GFP fusion protein localizes to the vacuolar membrane in root and leaf cells”
Claim · model-written summary
Promoter-GUS analysis suggests AtMTP1 is not produced throughout the plant but primarily in dividing, differentiating and expanding cells.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Organ / stage
- dividing, differentiating and expanding cellse1
- Gene
- AtMTP1e1
- Outcome
- not produced throughout the plante1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana”
e1“AtMTP1 is not produced throughout the plant, but primarily in the subpopulation of dividing, differentiating and expanding cells”
Claim · model-written summary
RNA interference-mediated silencing of AtMTP1 causes Zn hypersensitivity in Arabidopsis.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- RNA interference-mediated silencing of AtMTP1e1
- Gene
- AtMTP1e1
- Outcome
- Zn hypersensitivitye1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana”
e1“RNA interference-mediated silencing of AtMTP1 causes Zn hypersensitivity and a reduction in Zn concentrations in vegetative plant tissues”
Claim · model-written summary
RNA interference-mediated silencing of AtMTP1 reduces Zn concentrations in vegetative plant tissues of Arabidopsis.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Organ / stage
- vegetative plant tissuese1
- Treatment
- RNA interference-mediated silencing of AtMTP1e1
- Gene
- AtMTP1e1
- Outcome
- Zn concentrationse1
- Direction
- reductione1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana”
e1“RNA interference-mediated silencing of AtMTP1 causes Zn hypersensitivity and a reduction in Zn concentrations in vegetative plant tissues”
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