Abstract
A protein kinase activity from spinach chloroplasts, tightly associated with the thylakoid membranes, has been solubilized and partially characterized. This membrane-bound protein kinase is stimulated by light and electron transport activity through photosystem II appears to be required for stimulation.Electron transport inhibitors like 3,4-dichlorophenyl-1,1-dimethylurea, Tris, and NH(2)OH treatments, inhibit the light activation process. Furthermore, after Tris inhibition, the protein kinase activity is restored by washing the Tris-treated chloroplasts with dichlorophenol indophenol plus ascorbate. The protein kinase remains active in the dark after short illumination periods, suggesting that a product of electron transport may be involved in light activation.Two endogenous substrates of the protein kinase in thylakoid membranes are the N,N'-dicyclohexylcarbodiimide-reactive proteolipid and the light-harvesting chlorophyll-protein complex. The membrane-bound protein kinase also phosphorylates externally added histone.
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Claims 9
Claim · model-written summary
A thylakoid-associated protein kinase was solubilized and partly characterized.
- Taxon
- spinach
Spinacia oleraceae0 - Organ / stage
- chloroplastse0
- Gene
- protein kinasee0
- Outcome
- protein kinase activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A protein kinase activity from spinach chloroplasts, tightly associated with the thylakoid membranes, has been solubilized and partially characterized”
Claim · model-written summary
Light stimulated membrane-bound protein kinase activity.
- Taxon
- spinachSpinacia oleraceat0
- Treatment
- lighte0
- Gene
- protein kinasee0
- Outcome
- protein kinasee0
- Direction
- stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This membrane-bound protein kinase is stimulated by light”
t0“spinach chloroplasts”
Claim · model-written summary
Photosystem II electron transport appeared necessary for light stimulation.
- Taxon
- spinachSpinacia oleraceat0
- Outcome
- stimulatione0
- Qualifier
- electron transport activity through photosystem IIe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“electron transport activity through photosystem II appears to be required for stimulation”
t0“spinach chloroplasts”
Claim · model-written summary
Electron transport inhibitors blocked light activation.
- Taxon
- spinachSpinacia oleraceat0
- Treatment
- Electron transport inhibitorse0
- Gene
- protein kinaset1
- Outcome
- light activation processe0
- Direction
- inhibite0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Electron transport inhibitors like 3,4-dichlorophenyl-1,1-dimethylurea, Tris, and NH(2)OH treatments, inhibit the light activation process”
t0“spinach chloroplasts”
t1“This membrane-bound protein kinase”
Claim · model-written summary
Dichlorophenol indophenol plus ascorbate washing restored kinase activity after Tris inhibition.
- Taxon
- spinachSpinacia oleraceat0
- Treatment
- washing the Tris-treated chloroplasts with dichlorophenol indophenol plus ascorbatee0
- Gene
- protein kinasee0
- Outcome
- protein kinase activitye0
- Direction
- restorede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“after Tris inhibition, the protein kinase activity is restored by washing the Tris-treated chloroplasts with dichlorophenol indophenol plus ascorbate”
t0“spinach chloroplasts”
Claim · model-written summary
The kinase remained active in darkness after brief illumination.
- Taxon
- spinachSpinacia oleraceat0
- Gene
- protein kinasee0
- Outcome
- protein kinase remains active in the darke0
- Qualifier
- after short illumination periodse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The protein kinase remains active in the dark after short illumination periods”
t0“spinach chloroplasts”
Claim · model-written summary
Electron transport product may participate in kinase light activation.
- Taxon
- spinachSpinacia oleraceat0
- Outcome
- light activatione0
- Qualifier
- product of electron transporte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suggesting that a product of electron transport may be involved in light activation”
t0“spinach chloroplasts”
Claim · model-written summary
The proteolipid and light-harvesting complex were endogenous kinase substrates.
- Taxon
- spinachSpinacia oleraceat0
- Gene
- protein kinasee0
- Outcome
- endogenous substratese0
- Qualifier
- N,N'-dicyclohexylcarbodiimide-reactive proteolipid and the light-harvesting chlorophyll-protein complexe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two endogenous substrates of the protein kinase in thylakoid membranes are the N,N'-dicyclohexylcarbodiimide-reactive proteolipid and the light-harvesting chlorophyll-protein complex”
t0“spinach chloroplasts”
Claim · model-written summary
The kinase phosphorylated externally added histone.
- Taxon
- spinachSpinacia oleraceat0
- Gene
- protein kinasee0
- Outcome
- phosphorylates externally added histonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The membrane-bound protein kinase also phosphorylates externally added histone”
t0“spinach chloroplasts”
The same questions in other papers
ascorbate · physiology: enzyme activity · Spinacia oleracea (spinach)Positive and negative results
1 in the opposite direction
- no effect
light · quality: protein · Spinacia oleracea (spinach)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.