Abstract
Rice, a salt-sensitive species has a substantial genetic variation for salt tolerance within the cultivated gene pool. This study describes the establishment of cell suspension culture system and analysis of proline content of the selected rice genotypes under in vitro salt stress. The cell suspension cultures, initiated from the friable seed-derived callus, were established in MS medium supplemented with different concentrations of 2,4-D (0-25 μM) and subjected to different concentrations of NaCl (0-250 mM). The average Settled Cell Volume (SCV) of different clumps sizes was recorded. The results showed that MR219-4 exhibited the highest cell growth rate and regeneration capacity. The 750 μm sieved clumps have higher cell mass than the 500 and 250 μm sieved cell size. The shoot-regeneration capacity was maximum in 1/2 MS medium supplemented with 10 μM BAP. However, low regeneration capacity was obtained in NaCl treatments. MR219 (control) and MR219-9 appeared to be more NaCl tolerant than MR219-4. In addition, all genotypes significantly synthesized proline under NaCl conditions as demonstrated by the proline accumulation and MR219-4 revealed the lowest content among genotypes studied. These genotypes can be good model systems for studying the physiological mechanisms associated with in vitro selection for salt stress. This finding also suggests that proline may play a crucial role in protecting rice cells under salinity stress.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 11
Claim · model-written summary
MR219-4 had the highest cell growth rate.
- Taxon
- Rice
Oryza sativat0 - Material
- MR219-4e0
- Outcome
- cell growth ratee0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MR219-4 exhibited the highest cell growth rate and regeneration capacity.”
t0“Rice”
Claim · model-written summary
MR219-4 had the highest regeneration capacity.
- Taxon
- RiceOryza sativat0
- Material
- MR219-4e0
- Outcome
- regeneration capacitye0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MR219-4 exhibited the highest cell growth rate and regeneration capacity.”
t0“Rice”
Claim · model-written summary
The 750 μm sieved clumps had higher cell mass than the 500 and 250 μm clumps.
- Taxon
- RiceOryza sativat0
- Material
- 750 μm sieved clumpse0
- Outcome
- cell masse0
- Direction
- highere0
- Comparator
- 500 and 250 μm sieved cell sizee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 750 μm sieved clumps have higher cell mass than the 500 and 250 μm sieved cell size.”
t0“Rice”
Claim · model-written summary
Shoot regeneration was maximum in half strength MS medium with 10 μM BAP.
- Taxon
- RiceOryza sativat0
- Treatment
- 1/2 MS medium supplemented with 10 μM BAPe0
- Outcome
- shoot-regeneration capacitye0
- Direction
- maximume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The shoot-regeneration capacity was maximum in 1/2 MS medium supplemented with 10 μM BAP.”
t0“Rice”
Claim · model-written summary
NaCl treatments gave low regeneration capacity.
- Taxon
- RiceOryza sativat0
- Treatment
- NaCl treatmentse0
- Outcome
- regeneration capacitye0
- Direction
- lowe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“However, low regeneration capacity was obtained in NaCl treatments.”
t0“Rice”
Claim · model-written summary
MR219 control appeared more NaCl tolerant than MR219-4.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MR219 (control) and MR219-9 appeared to be more NaCl tolerant than MR219-4.”
t0“Rice”
Claim · model-written summary
MR219-9 appeared more NaCl tolerant than MR219-4.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MR219 (control) and MR219-9 appeared to be more NaCl tolerant than MR219-4.”
t0“Rice”
Claim · model-written summary
All rice genotypes accumulated proline significantly under NaCl conditions.
- Taxon
- RiceOryza sativat0
- Treatment
- NaCl conditionse0
- Outcome
- proline accumulatione0
- Direction
- accumulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“all genotypes significantly synthesized proline under NaCl conditions as demonstrated by the proline accumulation”
t0“Rice”
Claim · model-written summary
MR219-4 had the lowest proline content among the genotypes.
- Taxon
- RiceOryza sativat0
- Material
- MR219-4e0
- Outcome
- proline accumulatione1
- Direction
- loweste0
- Comparator
- genotypes studiede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MR219-4 revealed the lowest content among genotypes studied.”
e1“all genotypes significantly synthesized proline under NaCl conditions as demonstrated by the proline accumulation”
t0“Rice”
Claim · model-written summary
The authors propose these rice genotypes as model systems for studying physiological mechanisms of in vitro salt selection.
- Taxon
- RiceOryza sativat0
- Outcome
- good model systems for studying the physiological mechanisms associated with in vitro selection for salt stresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These genotypes can be good model systems for studying the physiological mechanisms associated with in vitro selection for salt stress.”
t0“Rice”
Claim · model-written summary
The authors suggest proline may protect rice cells under salinity stress.
- Taxon
- RiceOryza sativat0
- Compound
- prolinee0
- Outcome
- protecting rice cells under salinity stresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“proline may play a crucial role in protecting rice cells under salinity stress.”
t0“Rice”
The same questions in other papers
N-benzyladenine · growth · Oryza sativa (rice)Results differ
67 papers in the same direction as this paper · 3 in the opposite direction · 1 reporting no difference from an alternative
- negative
- negative
- negative
68 papers more in this group
See all 72 papers →sodium chloride · growth · Oryza sativa (rice)Results differ
99 papers in the same direction as this paper · 28 in the opposite direction
- positive
- positive
- positive
124 papers more in this group
See all 128 papers →sodium chloride · physiology: osmolytes · Oryza sativa (rice)Positive and negative results
8 papers in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
7 papers more in this group
See all 11 papers →in vitro · growth · Oryza sativa (rice)Positive and negative results
139 papers in the same direction as this paper · 24 in the opposite direction · 1 reporting no difference from an alternative
- negative
- negative
- negative
161 papers more in this group
See all 165 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.